scholarly journals Microbial microdroplet culture system (MMC): An integrated platform for automated, high‐throughput microbial cultivation and adaptive evolution

2020 ◽  
Vol 117 (6) ◽  
pp. 1724-1737 ◽  
Author(s):  
Xingjin Jian ◽  
Xiaojie Guo ◽  
Jia Wang ◽  
Zheng Lin Tan ◽  
Xin‐hui Xing ◽  
...  
Keyword(s):  
Adaptive Evolution ◽  
High Throughput ◽  
Culture System ◽  
Integrated Platform ◽  
Microbial Cultivation

scholarly journals Microbial microdroplet culture system (MMC): an integrated platform for automated, high–throughput microbial cultivation and adaptive evolution

2019 ◽  
Author(s):  
Xingjin Jian ◽  
Xiaojie Guo ◽  
Jia Wang ◽  
Zheng Lin Tan ◽  
Xin-hui Xing ◽  
...  
Keyword(s):  
Adaptive Evolution ◽  
High Throughput ◽  
Culture System ◽  
Growth Curves ◽  
Droplet Microfluidics ◽  
Microbial Cell ◽  
Superior Performance ◽  
Cell Cultivation ◽  
Integrated Platform ◽  
Microbial Cultivation

AbstractConventional microbial cell cultivation techniques are typically labor intensive, low throughput, and poor parallelization, rendering them inefficient. The development of automated, modular microbial cell micro–cultivation systems, particularly those employing droplet microfluidics, has gained attention for their high–throughput, parallel and highly efficient cultivation capabilities. Here, we report the development of a microbial microdroplet culture system (MMC), which is an integrated platform for automated, high–throughput cultivation and adaptive evolution of microorganisms. We demonstrated that the MMC yielded both accurate and reproducible results for the manipulation and detection of droplets. The superior performance of MMC for microbial cell cultivation was validated by comparing the growth curves of six microbial strains grown in MMC, conventional shake flasks or well plates. The highest incipient growth rate for all six microbial cell lines was achieved using MMC. We also conducted an 18–day process of adaptive evolution of a methanol–essential Escherichia coli strain in MMC and obtained two strains exhibiting higher growth rates compared with the parent strain. Our study demonstrates the power of MMC to provide an efficient and reliable approach for automated, high–throughput microbial cultivation and adaptive evolution.

An integrated platform for a high-throughput pharmacokinetic study of glycosides using a boronic acid-functionalized 96-well glass plate

2019 ◽  
Vol 55 (64) ◽  
pp. 9543-9546
Author(s):  
Ningning Zhao ◽  
Qianqian Gu ◽  
Zhiqiang Liu ◽  
Fengrui Song ◽  
Zifeng Pi ◽  
...  
Keyword(s):  
High Throughput ◽  
Pharmacokinetic Study ◽  
Boronic Acid ◽  
Large Scale ◽  
Glass Plate ◽  
The Novel ◽  
Ms Analysis ◽  
Integrated Platform

The novel Vial@FPBA strategy was established for a large-scale pharmacokinetic study of glycosides, during which glycosides were absorbed into a boronic acid-functionalized 96-well glass plate and directly desorbed for UHPLC-MS/MS analysis.

A high-throughput cell culture system based on capillary and centrifugal actions for rapid antimicrobial susceptibility testing

Lab on a Chip ◽  
2020 ◽  
Vol 20 (24) ◽  
pp. 4552-4560
Author(s):  
Taegeun Lim ◽  
Eun-Geun Kim ◽  
Jungil Choi ◽  
Sunghoon Kwon
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Antimicrobial Susceptibility ◽  
Culture System ◽  
Susceptibility Testing ◽  
Culture Media ◽  
Antimicrobial Susceptibility Testing ◽  
Testing System ◽  
Cell Culture System

A capillary and centrifuge-based rapid antimicrobial susceptibility testing system is developed to reduce the time of loading the sample and culture media while achieving a high-throughput testing capacity.

An integrated platform for fully automated high-throughput LC–MS/MS analysis of in vitro metabolic stability assay samples

2010 ◽  
Vol 296 (1-3) ◽  
pp. 1-9 ◽  
Author(s):  
Andreas H. Luippold ◽  
Thomas Arnhold ◽  
Wolfgang Jörg ◽  
Roderich D. Süssmuth
Keyword(s):  
High Throughput ◽  
Metabolic Stability ◽  
Ms Analysis ◽  
Integrated Platform

pH measurement and a rational and practical pH control strategy for high throughput cell culture system

2009 ◽  
Vol 26 (3) ◽  
pp. 872-880 ◽  
Author(s):  
Haiying Zhou ◽  
Jennifer Purdie ◽  
Tongtong Wang ◽  
Anli Ouyang
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Control Strategy ◽  
Culture System ◽  
Ph Control ◽  
Ph Measurement ◽  
Cell Culture System

scholarly journals CNV Workshop: an integrated platform for high-throughput copy number variation discovery and clinical diagnostics

2010 ◽  
Vol 11 (1) ◽  
pp. 74 ◽  
Author(s):  
Xiaowu Gai ◽  
Juan C Perin ◽  
Kevin Murphy ◽  
Ryan O'Hara ◽  
Monica D'arcy ◽  
...  
Keyword(s):  
Copy Number Variation ◽  
High Throughput ◽  
Copy Number ◽  
Clinical Diagnostics ◽  
Integrated Platform ◽  
Number Variation

scholarly journals X-CHIP: An Integrated Platform for High-Throughput Protein Crystallography

10.5772/48581 ◽  
2012 ◽  
Author(s):  
Nickolay Y. ◽  
Gera Kisselman ◽  
Wei Qiu ◽  
Vladimir Romanov ◽  
Christine M. ◽  
...  
Keyword(s):  
High Throughput ◽  
Protein Crystallography ◽  
Integrated Platform
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