A high-throughput cell culture system based on capillary and centrifugal actions for rapid antimicrobial susceptibility testing

Lab on a Chip ◽  
2020 ◽  
Vol 20 (24) ◽  
pp. 4552-4560
Author(s):  
Taegeun Lim ◽  
Eun-Geun Kim ◽  
Jungil Choi ◽  
Sunghoon Kwon
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Antimicrobial Susceptibility ◽  
Culture System ◽  
Susceptibility Testing ◽  
Culture Media ◽  
Antimicrobial Susceptibility Testing ◽  
Testing System ◽  
Cell Culture System

A capillary and centrifuge-based rapid antimicrobial susceptibility testing system is developed to reduce the time of loading the sample and culture media while achieving a high-throughput testing capacity.

An automated and portable antimicrobial susceptibility testing system for urinary tract infections

Lab on a Chip ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 755-763
Author(s):  
Kuo-Wei Hsu ◽  
Wen-Bin Lee ◽  
Huey-Ling You ◽  
Mel S. Lee ◽  
Gwo-Bin Lee
Keyword(s):  
Urinary Tract ◽  
Minimum Inhibitory Concentration ◽  
Urinary Tract Infections ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Inhibitory Concentration ◽  
Microfluidic System ◽  
Antimicrobial Susceptibility Testing ◽  
Testing System ◽  
Tract Infections

A portable, integrated microfluidic system capable of automatically conducting antimicrobial susceptibility testing (AST) and minimum inhibitory concentration (MIC) measurements using urine samples were developed.

pH measurement and a rational and practical pH control strategy for high throughput cell culture system

2009 ◽  
Vol 26 (3) ◽  
pp. 872-880 ◽  
Author(s):  
Haiying Zhou ◽  
Jennifer Purdie ◽  
Tongtong Wang ◽  
Anli Ouyang
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Control Strategy ◽  
Culture System ◽  
Ph Control ◽  
Ph Measurement ◽  
Cell Culture System

Hydrogel-incorporating unit in a well: 3D cell culture for high-throughput analysis

Lab on a Chip ◽  
2018 ◽  
Vol 18 (17) ◽  
pp. 2604-2613 ◽  
Author(s):  
Yeong Jun Yu ◽  
Young Hye Kim ◽  
Kyuhwan Na ◽  
Seo Yun Min ◽  
Ok Kyung Hwang ◽  
...  
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Culture System ◽  
3D Cell Culture ◽  
High Content Screening ◽  
Cell Culture System ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Biochemical Assays

A microchannel-free, 3D cell culture system has a hydrogel-incorporating unit integrated with a multi-well plate. This plate provides better reproducibility in a variety of quantitative biochemical assays and high content-screening (HCS).

scholarly journals High-Throughput Intracellular Antimicrobial Susceptibility Testing of Legionella pneumophila

2015 ◽  
Vol 59 (12) ◽  
pp. 7517-7529 ◽  
Author(s):  
Lucius Chiaraviglio ◽  
James E. Kirby
Keyword(s):  
High Resolution ◽  
Growth Inhibition ◽  
High Throughput ◽  
Antimicrobial Susceptibility ◽  
Legionella Pneumophila ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Response Analysis ◽  
Intracellular Growth ◽  
Macrophage Infection

ABSTRACTLegionella pneumophilais a Gram-negative opportunistic human pathogen that causes a severe pneumonia known as Legionnaires' disease. Notably, in the human host, the organism is believed to replicate solely within an intracellular compartment, predominantly within pulmonary macrophages. Consequently, successful therapy is predicated on antimicrobials penetrating into this intracellular growth niche. However, standard antimicrobial susceptibility testing methods test solely for extracellular growth inhibition. Here, we make use of a high-throughput assay to characterize intracellular growth inhibition activity of known antimicrobials. For select antimicrobials, high-resolution dose-response analysis was then performed to characterize and compare activity levels in both macrophage infection and axenic growth assays. Results support the superiority of several classes of nonpolar antimicrobials in abrogating intracellular growth. Importantly, our assay results show excellent correlations with prior clinical observations of antimicrobial efficacy. Furthermore, we also show the applicability of high-throughput automation to two- and three-dimensional synergy testing. High-resolution isocontour isobolograms providein vitrosupport for specific combination antimicrobial therapy. Taken together, findings suggest that high-throughput screening technology may be successfully applied to identify and characterize antimicrobials that target bacterial pathogens that make use of an intracellular growth niche.

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