scholarly journals A yeast optogenetic toolkit (yOTK) for gene expression control in Saccharomyces cerevisiae

2019 ◽  
Vol 117 (3) ◽  
pp. 886-893 ◽  
Author(s):  
Jidapas (My) An‐adirekkun ◽  
Cameron J. Stewart ◽  
Stephanie H. Geller ◽  
Michael T. Patel ◽  
Justin Melendez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Expression Control ◽  
Gene Expression Control

scholarly journals A yeast optogenetic toolkit (yOTK) for gene expression control in Saccharomyces cerevisiae

2019 ◽  
Author(s):  
Jidapas (My) An-adirekkun ◽  
Cameron J. Stewart ◽  
Stephanie H. Geller ◽  
Michael T. Patel ◽  
Justin Melendez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Biological Networks ◽  
Modular Assembly ◽  
Expression Control ◽  
Control Gene Expression ◽  
Gene Expression Control ◽  
Rapid Generation ◽  
Spatiotemporal Control ◽  
Different Levels

AbstractOptogenetic tools for controlling gene expression are ideal for tuning synthetic biological networks due to the exquisite spatiotemporal control available with light. Here we develop an optogenetic system for gene expression control and integrate it with an existing yeast toolkit allowing for rapid, modular assembly of light-controlled circuits in the important chassis organism Saccharomyces cerevisiae. We reconstitute activity of a split synthetic zinc-finger transcription factor (TF) using light-induced dimerization. We optimize function of this split TF and demonstrate the utility of the toolkit workflow by assembling cassettes expressing the TF activation domain and DNA-binding domain at different levels. Utilizing this TF and a synthetic promoter we demonstrate that light-intensity and duty-cycle can be used to modulate gene expression over the range currently available from natural yeast promoters. This work allows for rapid generation and prototyping of optogenetic circuits to control gene expression in Saccharomyces cerevisiae.

scholarly journals High-performance chemical- and light-inducible recombinases in mammalian cells and mice

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Benjamin H. Weinberg ◽  
Jang Hwan Cho ◽  
Yash Agarwal ◽  
N. T. Hang Pham ◽  
Leidy D. Caraballo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammalian Cells ◽  
High Performance ◽  
Genome Engineering ◽  
Genetic Circuits ◽  
Control Of Gene Expression ◽  
Expression Control ◽  
Gene Expression Control ◽  
High Performance Systems ◽  
Spatiotemporal Control

Abstract Site-specific DNA recombinases are important genome engineering tools. Chemical- and light-inducible recombinases, in particular, enable spatiotemporal control of gene expression. However, inducible recombinases are scarce due to the challenge of engineering high performance systems, thus constraining the sophistication of genetic circuits and animal models that can be created. Here we present a library of >20 orthogonal inducible split recombinases that can be activated by small molecules, light and temperature in mammalian cells and mice. Furthermore, we engineer inducible split Cre systems with better performance than existing systems. Using our orthogonal inducible recombinases, we create a genetic switchboard that can independently regulate the expression of 3 different cytokines in the same cell, a tripartite inducible Flp, and a 4-input AND gate. We quantitatively characterize the inducible recombinases for benchmarking their performances, including computation of distinguishability of outputs. This library expands capabilities for multiplexed mammalian gene expression control.

scholarly journals Global quantification of mammalian gene expression control

Nature ◽  
2011 ◽  
Vol 473 (7347) ◽  
pp. 337-342 ◽  
Author(s):  
Björn Schwanhäusser ◽  
Dorothea Busse ◽  
Na Li ◽  
Gunnar Dittmar ◽  
Johannes Schuchhardt ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammalian Gene ◽  
Expression Control ◽  
Gene Expression Control ◽  
Mammalian Gene Expression

The ins and outs of gene expression control

2004 ◽  
Vol 22 (7) ◽  
pp. 824-826 ◽  
Author(s):  
Francine B Perler
Keyword(s):  
Gene Expression ◽  
Expression Control ◽  
Gene Expression Control
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