Biosynthesis of the human milk oligosaccharide 3‐fucosyllactose in metabolically engineered Escherichia coli via the salvage pathway through increasing GTP synthesis and β‐galactosidase modification

2019 ◽  
Vol 116 (12) ◽  
pp. 3324-3332 ◽  
Author(s):  
Yun Hee Choi ◽  
Bum Seok Park ◽  
Joo‐Hyun Seo ◽  
Byung‐Gee Kim
Keyword(s):  
Escherichia Coli ◽  
Human Milk ◽  
Salvage Pathway ◽  
Milk Oligosaccharide ◽  
Human Milk Oligosaccharide ◽  
Engineered Escherichia Coli ◽  
Metabolically Engineered Escherichia Coli

High-Titer De Novo Biosynthesis of the Predominant Human Milk Oligosaccharide 2′-Fucosyllactose from Sucrose in Escherichia coli

2020 ◽  
Vol 9 (10) ◽  
pp. 2784-2796
Author(s):  
Katja Parschat ◽  
Sandra Schreiber ◽  
Dirk Wartenberg ◽  
Benedikt Engels ◽  
Stefan Jennewein
Keyword(s):  
Escherichia Coli ◽  
Human Milk ◽  
De Novo ◽  
High Titer ◽  
Milk Oligosaccharide ◽  
De Novo Biosynthesis ◽  
Human Milk Oligosaccharide

scholarly journals Human Milk Oligosaccharide Profile Variation Throughout Postpartum in Healthy Women in a Brazilian Cohort

Nutrients ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 790 ◽  
Author(s):  
Ana Lorena Ferreira ◽  
Ronaldo Alves ◽  
Amanda Figueiredo ◽  
Nadya Alves-Santos ◽  
Nathalia Freitas-Costa ◽  
...  
Keyword(s):  
Human Milk ◽  
Rank Correlation ◽  
Postpartum Visit ◽  
Maternal Characteristics ◽  
Milk Oligosaccharide ◽  
Healthy Women ◽  
Human Milk Oligosaccharide ◽  
Post Hoc ◽  
Brazilian Cohort ◽  
Non Negative Matrix Factorization

Human milk oligosaccharide (HMO) composition varies throughout lactation and can be influenced by maternal characteristics. This study describes HMO variation up to three months postpartum and explores the influences of maternal sociodemographic and anthropometric characteristics in a Brazilian prospective cohort. We followed 101 subjects from 28–35 gestational weeks (baseline) and throughout lactation at 2–8 (visit 1), 28–50 (visit 2) and 88–119 days postpartum (visit 3). Milk samples were collected at visits 1, 2 and 3, and 19 HMOs were quantified usinghigh-performance liquid chromatography with fluorescence detection (HPLC-FL). Friedman post-hoc test, Spearman rank correlation for maternal characteristics and HMOs and non-negative matrix factorization (NMF) were used to define the HMO profile. Most women were secretors (89.1%) and presented high proportion of 2′-fucosyllactose (2′FL) at all three sample times, while lacto-N-tetraose (LNT, 2–8 days) and lacto-N-fucopentaose II (LNFPII, 28–50 and 88–119 days) were the most abundant HMOs in non-secretor women. Over the course of lactation, total HMO weight concentrations (g/L) decreased, but total HMO molar concentrations (mmol/L) increased, highlighting differential changes in HMO composition over time. In addition, maternal pre-pregnancy body mass index (BMI) and parity influence the HMO composition in healthy women in this Brazilian cohort.

scholarly journals Human norovirus inhibition by a human milk oligosaccharide

Virology ◽  
2017 ◽  
Vol 508 ◽  
pp. 81-89 ◽  
Author(s):  
Anna Koromyslova ◽  
Shailesh Tripathi ◽  
Vasily Morozov ◽  
Horst Schroten ◽  
Grant S. Hansman
Keyword(s):  
Human Milk ◽  
Human Norovirus ◽  
Milk Oligosaccharide ◽  
Human Milk Oligosaccharide
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