Enzymatic preparation of biosurfactants from sugars or sugar alcohols and fatty acids in organic media under reduced pressure

1995 ◽  
Vol 48 (3) ◽  
pp. 214-221 ◽  
Author(s):  
Am�lie Ducret ◽  
Andr� Giroux ◽  
Michael Trani ◽  
Robert Lortie
Keyword(s):  
Fatty Acids ◽  
Organic Media ◽  
Enzymatic Preparation ◽  
Sugar Alcohols ◽  
Reduced Pressure

Convenient biosynthetic preparation of isomeric spin-labelled radioactive phosphatidic acids

1982 ◽  
Vol 60 (11) ◽  
pp. 1014-1017 ◽  
Author(s):  
L. Stuhne-Sekalec ◽  
N. Z. Stanacev
Keyword(s):  
Fatty Acids ◽  
Guinea Pig ◽  
Stearic Acid ◽  
Liver Microsomes ◽  
Enzymatic Preparation ◽  
Pig Liver ◽  
Secondary Hydroxyl ◽  
Phosphatidic Acids ◽  
Guinea Pig Liver Microsomes ◽  
Guinea Pig Liver

A convenient method for the enzymatic preparation of sn-3-[2-3H]phosphatidic acids carrying also 5-, 12-, or 16-nitroxide stearic acids, from sn-3-[2-3H]glycerophosphate and isolated guinea pig liver microsomes, is described in detail. The procedure allows a simultaneous preparation of three spin-labelled sn-3-[2-3H] phosphatidic acids of yields 3–3.5 μmol of each compound which is > 99% pure in respect to the radioactivity and which contains 25 mol% of spin-labelled fatty acids. These phosphatidic acids were approximately equally distributed between the primary and the secondary hydroxyl when 12- or 16-nitroxide stearic acids were used or predominantly (75%) associated with the secondary hydroxyl of sn-3-[2-3H]phosphatidic acid when 5-nitroxide stearic acid was present in the incubation mixture.

Chemical and enzymatic preparation of acylglycerols containing C18 furanoid fatty acids

Lipids ◽  
1995 ◽  
Vol 30 (1) ◽  
pp. 79-84 ◽  
Author(s):  
Marcel S. F. Lie Ken Jie ◽  
M. S. K. Syed-Rahmatullah
Keyword(s):  
Fatty Acids ◽  
Enzymatic Preparation ◽  
Furanoid Fatty Acids

ChemInform Abstract: Enzymatic Preparation of Optically Active Fungicide Intermediates in Aqueous and in Organic Media.

ChemInform ◽  
2010 ◽  
Vol 24 (18) ◽  
pp. no-no
Author(s):  
D. BIANCHI ◽  
P. CESTI ◽  
P. GOLINI ◽  
S. SPEZIA ◽  
C. GARAVAGLIA ◽  
...  
Keyword(s):  
Optically Active ◽  
Organic Media ◽  
Enzymatic Preparation

scholarly journals Enzymatic Preparation of Structured Oils Containing Short-Chain Fatty Acids

2010 ◽  
Vol 59 (12) ◽  
pp. 641-645 ◽  
Author(s):  
Ayato Kanda ◽  
Fusako Namiki ◽  
Setsuko Hara
Keyword(s):  
Fatty Acids ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Enzymatic Preparation ◽  
Chain Fatty Acids

Enzymatic preparation of optically active fungicide intermediates in aqueous and in organic media

1992 ◽  
Vol 64 (8) ◽  
pp. 1073-1078 ◽  
Author(s):  
D. Bianchi ◽  
Pietro Cesti ◽  
Paolo Golini ◽  
S. Spezia ◽  
Carlo Garavaglia ◽  
...  
Keyword(s):  
Optically Active ◽  
Organic Media ◽  
Enzymatic Preparation

PHYSIOLOGY OF WOOD-ROTTING BASIDIOMYCETES: IV. RESPIRATION OF NON-PROLIFERATING CELLS OF POLYPORUS PALUSTRIS

1962 ◽  
Vol 8 (1) ◽  
pp. 145-156 ◽  
Author(s):  
Harvey R. Newcomb ◽  
Marshall W. Jennison
Keyword(s):  
Carbon Dioxide ◽  
Fatty Acids ◽  
Anaerobic Metabolism ◽  
Aerobic Respiration ◽  
Endogenous Respiration ◽  
Shake Culture ◽  
Sugar Alcohols ◽  
Proliferating Cells ◽  
Mycelial Pellets ◽  
Manometric Studies

For manometric studies of respiration, small, whole mycelial pellets of Polyporus palustris grown in submerged (shake) culture were much superior to fragmented mycelium. The rates of endogenous and of exogenous respiration were dependent, in part, upon age of culture, and were maximal at pH 5.5. The endogenous respiration was high but could be reduced by starvation (aeration in a non-nutrient medium), permitting measurement of exogenous respiration upon the addition of oxidizable substrate. Of the carbohydrates studied, xylose, glucose, galactose, and sucrose were the most stimulative to aerobic respiration; rhamnose, melezitose, and lactose either were not oxidized or showed only slight stimulation. The alcohols methanol, ethanol, glycerol, and propylene glycol were readily utilized; inositol was oxidized to a lesser degree. The sugar alcohols mannitol, sorbitol, and adonitol were mildly stimulative; the effect of dulcitol was dependent upon its concentration. The fatty acids acetate and caprylate were much more readily utilized than butyrate. Salts of other acids— oxalate, pyruvate, lactate, and fumarate—were oxidized to about the same extent as butyrate. Non-proliferating cells of P. palustris had a definite anaerobic metabolism and fermented glucose with production of carbon dioxide and small amounts of acid(s). In air, oxidative assimilation of glucose was high (79–91%); endogenous respiration apparently was not suppressed during assimilation.

scholarly journals Environmental Metabolomics of the Tomato Plant Surface Provides Insights on Salmonella enterica Colonization

2016 ◽  
Vol 82 (10) ◽  
pp. 3131-3142 ◽  
Author(s):  
Sanghyun Han ◽  
Shirley A. Micallef
Keyword(s):  
Fatty Acids ◽  
Organic Acids ◽  
Fresh Produce ◽  
Enteric Bacteria ◽  
Plant Surface ◽  
Sugar Alcohols ◽  
Content Type ◽  
Surface Compounds ◽  
Plant Organ ◽  
Environmental Metabolomics

ABSTRACTFoodborne illness-causing enteric bacteria are able to colonize plant surfaces without causing infection. We lack an understanding of how epiphytic persistence of enteric bacteria occurs on plants, possibly as an adaptive transit strategy to maximize chances of reentering herbivorous hosts. We used tomato (Solanum lycopersicum) cultivars that have exhibited differential susceptibilities toSalmonella entericacolonization to investigate the influence of plant surface compounds and exudates on enteric bacterial populations. Tomato fruit, shoot, and root exudates collected at different developmental stages supported growth ofS. entericato various degrees in a cultivar- and plant organ-dependent manner.S. entericagrowth in fruit exudates of various cultivars correlated with epiphytic growth data (R2= 0.504;P= 0.006), providing evidence that plant surface compounds drive bacterial colonization success. Chemical profiling of tomato surface compounds with gas chromatography-time of flight mass spectrometry (GC-TOF-MS) provided valuable information about the metabolic environment on fruit, shoot, and root surfaces. Hierarchical cluster analysis of the data revealed quantitative differences in phytocompounds among cultivars and changes over a developmental course and by plant organ (P< 0.002). Sugars, sugar alcohols, and organic acids were associated with increasedS. entericagrowth, while fatty acids, including palmitic and oleic acids, were negatively correlated. We demonstrate that the plant surface metabolite landscape has a significant impact onS. entericagrowth and colonization efficiency. This environmental metabolomics approach provides an avenue to understand interactions between human pathogens and plants that could lead to strategies to identify or breed crop cultivars for microbiologically safer produce.IMPORTANCEIn recent years, fresh produce has emerged as a leading food vehicle for enteric pathogens.Salmonella-contaminated tomatoes represent a recurrent human pathogen-plant commodity pair. We demonstrate thatSalmonellacan utilize tomato surface compounds and exudates for growth. Surface metabolite profiling revealed that the types and amounts of compounds released to the plant surface differ by cultivar, plant developmental stage, and plant organ. Differences in exudate profiles explain some of the variability inSalmonellacolonization susceptibility seen among tomato cultivars. Certain medium- and long-chain fatty acids were associated with restrictedSalmonellagrowth, while sugars, sugar alcohols, and organic acids correlated with largerSalmonellapopulations. These findings uncover the possibility of selecting crop varieties based on characteristics that impair foodborne pathogen growth for enhanced safety of fresh produce.

scholarly journals Enzymatic preparation of phytosterol esters with fatty acids from high-oleic sunflower seed oil using response surface methodology

RSC Advances ◽  
2021 ◽  
Vol 11 (25) ◽  
pp. 15204-15212
Author(s):  
Xiaoping Wang ◽  
Bing Xiao ◽  
Guolong Yang ◽  
Jingnan Chen ◽  
Wei Liu
Keyword(s):  
Fatty Acids ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Sunflower Seed ◽  
Seed Oil ◽  
Enzymatic Preparation ◽  
Reaction Conditions ◽  
High Oleic ◽  
Sunflower Seed Oil ◽  
Phytosterol Esters

Enzymatic preparation of phytosterol esters with fatty acids from high-oleic sunflower seed oil has been established with very mild reaction conditions.

Epoxidation of Fatty Acids by Pluronic-Conjugated Lipase in Organic Media

2016 ◽  
Vol 146 (6) ◽  
pp. 1073-1078 ◽  
Author(s):  
Oda Yuki ◽  
Yifei Zhang ◽  
Jun Ge ◽  
Zheng Liu
Keyword(s):  
Fatty Acids ◽  
Organic Media
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