Observing protein synthesis, export, and tryptophan incorporation by front-surface fluorescence

1992 ◽  
Vol 39 (1) ◽  
pp. 13-19 ◽  
Author(s):  
A. J. Lipton ◽  
M. M. Domach
Keyword(s):  
Protein Synthesis ◽  
Front Surface ◽  
Surface Fluorescence

Radial partition immunoassay.

1982 ◽  
Vol 28 (9) ◽  
pp. 1894-1898 ◽  
Author(s):  
J L Giegel ◽  
M M Brotherton ◽  
P Cronin ◽  
M D'Aquino ◽  
S Evans ◽  
...  
Keyword(s):  
Glass Fiber ◽  
Filter Paper ◽  
Central Area ◽  
Enzyme Reaction ◽  
Front Surface ◽  
Testing Procedure ◽  
Testing Time ◽  
Surface Fluorescence ◽  
Automated Instrument ◽  
Total Testing Time

Abstract In radial partition immunoassay, radial chromatography is used for performing an immunoassay. We describe the application of this technology to the measurement of digoxin in serum by enzyme immunoassay, with the entire testing procedure carried out on glass-fiber filter paper. A sample is applied to a small central area of the filter paper, where it reacts with the antibody to digoxin immobilized there. Subsequently, enzyme-labeled digoxin is applied to react with remaining antibody sites. After incubation, substrate for the enzyme is applied to the center of the reaction area and washes out any unbound label to the periphery of the paper. This step also initiates the enzyme reaction, which is quantified by front-surface fluorescence. A microprocessor-controlled automated instrument has been developed to process the filter paper matrix through the above sequence, and calculate the final result. Total testing time for digoxin is less than 7 min.

Concentration Dependence in the Spectra of Polycyclic Aromatic Hydrocarbon Mixtures by Front-Surface Fluorescence Analysis

2007 ◽  
Vol 61 (9) ◽  
pp. 970-977 ◽  
Author(s):  
Joseph F. Sinski ◽  
Jeffrey Exner
Keyword(s):  
Fluorescence Analysis ◽  
Front Surface ◽  
Hydrocarbon Mixtures ◽  
Energy Transfers ◽  
Study Results ◽  
Surface Fluorescence ◽  
Polycyclic Aromatic ◽  
Inner Filter Effects ◽  
High Concentrations ◽  
Filter Effects

Polycyclic aromatic hydrocarbons (PAHs) are a challenge for fluorescence analysis. Inner-filter quenching effects and energy transfers of PAHs in petroleum differ with concentration, leading to characteristically different fluorescence. At high concentrations of single PAH molecules and petroleum mixtures the classic rectangular quartz cell presents a particular challenge due to inner filter effects from right-angle illumination. While quite a few instrumental and mathematical corrections for these effects are reported throughout the literature, for researchers with standard instrumental sample compartments a triangular fluorometer cell can be used to emulate front-surface fluorescence analysis. This work presents concentration study results from the single PAH molecules fluoranthene and azulene dissolved in spectral-grade cyclohexane and also seeks to correct an aspect of our past work on the fluorescence of petroleum mixtures.

scholarly journals Conformational Changes of Whey and Pea Proteins upon Emulsification Approached by Front-Surface Fluorescence

2021 ◽  
Author(s):  
Emma B. A. Hinderink ◽  
Claire C. Berton-Carabin ◽  
Karin Schroën ◽  
Alain Riaublanc ◽  
Bérénice Houinsou-Houssou ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Front Surface ◽  
Surface Fluorescence ◽  
Pea Proteins

Homochirality as the Signature of Extra-Terrestrial Life

1997 ◽  
Vol 161 ◽  
pp. 505-510
Author(s):  
Alexandra J. MacDermott ◽  
Laurence D. Barron ◽  
Andrè Brack ◽  
Thomas Buhse ◽  
John R. Cronin ◽  
...  
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Optical Rotation ◽  
Physical Origin ◽  
Natural Choice ◽  
Rosetta Mission ◽  
Terrestrial Life ◽  
Subsurface Layers ◽  
Solar System Bodies ◽  
Origin Of Homochirality

AbstractThe most characteristic hallmark of life is its homochirality: all biomolecules are usually of one hand, e.g. on Earth life uses only L-amino acids for protein synthesis and not their D mirror images. We therefore suggest that a search for extra-terrestrial life can be approached as a Search for Extra- Terrestrial Homochirality (SETH). The natural choice for a SETH instrument is optical rotation, and we describe a novel miniaturized space polarimeter, called the SETH Cigar, which could be used to detect optical rotation as the homochiral signature of life on other planets. Moving parts are avoided by replacing the normal rotating polarizer by multiple fixed polarizers at different angles as in the eye of the bee. We believe that homochirality may be found in the subsurface layers on Mars as a relic of extinct life, and on other solar system bodies as a sign of advanced pre-biotic chemistry. We discuss the chiral GC-MS planned for the Roland lander of the Rosetta mission to a comet and conclude with theories of the physical origin of homochirality.

Lymphocyte activity and protein synthesis

2001 ◽  
Vol 101 (6) ◽  
pp. 591
Author(s):  
DEREK C. MACALLAN
Keyword(s):  
Protein Synthesis ◽  
Lymphocyte Activity

Protein synthesis in mitochondria purified from roots, leaves and flowers of sugar beet

1991 ◽  
Vol 83 (1) ◽  
pp. 7-16 ◽  
Author(s):  
Christina Lind ◽  
Christer Hallden ◽  
Ian M. Moller
Keyword(s):  
Protein Synthesis ◽  
Sugar Beet

Respiratory energy requirements and rate of protein turnover in vivo determined by the use of an inhibitor of protein synthesis and a probe to assess its effect

1994 ◽  
Vol 92 (4) ◽  
pp. 585-594 ◽  
Author(s):  
T. J. Bouma ◽  
R. De Visser ◽  
J. H. J. A. Janssen ◽  
M. J. De Kock ◽  
P H. Van Leeuwen ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Protein Turnover ◽  
Energy Requirements ◽  
Inhibitor Of Protein Synthesis
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