scholarly journals Large-Scale, High-density freezing of hybridomas and its application to high-density culture

1991 ◽  
Vol 38 (9) ◽  
pp. 1110-1113 ◽  
Author(s):  
Nobutaka Ninomiya ◽  
Sanetaka Shirahata ◽  
Hiroki Murakami ◽  
Takuya Sugahara
Keyword(s):  
Large Scale ◽  
High Density ◽  
High Density Culture

Large‐scale high‐density culture of hepatocytes in a liver microsystem with mimicked sinusoid blood flow

2018 ◽  
Vol 12 (12) ◽  
pp. 2266-2276
Author(s):  
Jing Liu ◽  
Chengpan Li ◽  
Shaohui Cheng ◽  
Shengnan Ya ◽  
Dayong Gao ◽  
...  
Keyword(s):  
Blood Flow ◽  
Large Scale ◽  
High Density ◽  
High Density Culture ◽  
Culture Of Hepatocytes

scholarly journals YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 3069
Author(s):  
Zheng Liu ◽  
Ling Lin ◽  
Haozhe Zhu ◽  
Zhongyuan Wu ◽  
Xi Ding ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Large Scale ◽  
High Density ◽  
Meat Production ◽  
Density Condition ◽  
High Density Culture ◽  
Differentiation Potential ◽  
Muscle Stem Cells ◽  
Cultured Meat

Muscle stem cells (MuSCs) isolated ex vivo are essential original cells to produce cultured meat. Currently, one of the main obstacles for cultured meat production derives from the limited capacity of large-scale amplification of MuSCs, especially under high-density culture condition. Here, we show that at higher cell densities, proliferation and differentiation capacities of porcine MuSCs are impaired. We investigate the roles of Hippo-YAP signaling, which is important regulators in response to cell contact inhibition. Interestingly, abundant but not functional YAP proteins are accumulated in MuSCs seeded at high density. When treated with lysophosphatidic acid (LPA), the activator of YAP, porcine MuSCs exhibit increased proliferation and elevated differentiation potential compared with control cells. Moreover, constitutively active YAP with deactivated phosphorylation sites, but not intact YAP, promotes cell proliferation and stemness maintenance of MuSCs. Together, we reveal a potential molecular target that enables massive MuSCs expansion for large-scale cultured meat production under high-density condition.

scholarly journals Enhancing Form-Based Code: A parametric approach to urban volumetric morphology in high density cities

2021 ◽  
Author(s):  
◽  
Yingyi Zhang
Keyword(s):  
Hong Kong ◽  
Large Scale ◽  
Positive Impact ◽  
High Density ◽  
Original Form ◽  
Human Society ◽  
Parametric Modelling ◽  
Physical Form ◽  
Urban Regulation ◽  
Modelling System

<p>Parametric tools have been broadly implemented in Architecture, Engineering and Construction (AEC) industry. Recently, an increasing volume of research finds that parametric tools also have the capability to facilitate large-scale planning and urban design. Much of this research, however, focuses on parametric representation or environment simulation. There is insufficient research about using parametric tools to enhance urban regulation. Parametric tools can provide smart design procedures by integrating strategies, solutions and expressions in one system. They may allow alternative approaches to urban regulation that conventional tools do not process.  This research aims to create a parametric modelling system to aid urban regulation. The system offers a visualised coding interface to manipulate parameters and achieve interactive performance feedback at the early stage of urban regulation. Form-Based Code uses the modelling system in this research. It generates a specific morphology by controlling physical form with less focus on land use. With the rise of New Urbanism, Form-Based Code has been used in various American regulation projects. This research extends the application of Form-Based Code, adopting it for urban-peripheral environments outside of the USA. High-density cities where provide the volumetric morphology context is important for this work. Tsim Sha Tsui area of Hong Kong works as an experimental site.  The feasibility of parametric urban regulation is examined by developing a parametric modelling system for Form-Based Code in Hong Kong. Understanding the site’s form characteristics, the transect matrix of Form-Based Code is expanded by incorporating multi-layered zone types and regulating plans. Embedding the zones into parametric modelling software Rhinoceros 3D and Grasshopper 3D, a regenerative prototype works to create real-time scenarios responding to parameters, rules and geometry constraints. The results of parametric urban regulation are evaluated by both Form-Based Code standards and local urban regulation standards to assess its feasibility in context.  This research demonstrates that the parametric modelling system for Form-Based Code has both technological and implemental potential to work as an alternative approach to urban regulation, especially in complex developments. Form complexity is a reflection of sophisticated human-society systems and the sequential evolution of a dynamic morphology. Form-Based Code is enhanced by the parametric modelling system to describe and regulate form complexity in a logical manner. Additionally, although parametric Form-Based Code processing is based on the original Form-Based Code, it is not limited to that. Describing urban regulation with visualised models bridges specialists and the public in community demonstrations and code assembling. The parametric modelling system has a positive impact on resolving challenges, predicting outcomes, and applying urban regulation innovation to the volumetric morphology of high-density cities in Asia.</p>

scholarly journals Hyper-intensive farming white shrimp Litopenaeus vannamei (Decapoda: Penaeidae) in a seawater tank under semi-controlled conditions

2013 ◽  
Vol 5 (1) ◽  
pp. 69-74
Author(s):  
A. A. Ortega-Salas ◽  
L. A. Rendón M.
Keyword(s):  
Litopenaeus Vannamei ◽  
Maximum Likelihood Method ◽  
High Density ◽  
White Shrimp ◽  
Likelihood Method ◽  
Food Conversion ◽  
High Density Culture ◽  
Intensive Farming ◽  
Controlled Conditions ◽  
Commercial Size

Shrimp development to a commercial size in high density culture saves food and avoids predators and disease. Our study was conducted to calculate the growth of white shrimp Litopenaeus vannamei by hyper-intensive cultivation under semi-controlled conditions. We seeded at a density of 550 shrimp per m3 during the first cycle and 400 shrimp per m3 in the second cycle in an outdoor tank of 6m3or 6m2 covered with mesh, constant aeration. The shrimp were fed Artemia franciscana during the first two weeks and camaronina pellets (35% protein) as required, in food baskets, aftterwards. The temperature ranged from 22,3 to 31,3°C, pH 7,5-8,7, oxygen 4,26±1,43. The tanks are siphoned of debris every other day, and water was replaced according to a program. The food conversion ratio (FCR) was 1:1,3. The shrimp were measured weekly to calculate growth with the Bertalanffy model. Survival in the first cycle was 95,8 ( 97,9% for the second cycle). Population parameters (maximum likelihood method) for the first cycle were k=0,0301, L∞ =322,16 and t0 =-0,8852; second cycle: k=0,0203, L∞ =294,42 and t0 =-5,3771. There was rapid growth during the first 10 weeks. Biomass was 27kg for the first cycle (second: 16kg). KEY WORDSGrowth, high density, survival, biomass, semi-controlled conditions. 

CO2 in large-scale and high-density CHO cell perfusion culture

1996 ◽  
Vol 22 (1-3) ◽  
pp. 65-78 ◽  
Author(s):  
David R. Gray ◽  
Su Chen ◽  
William Howarth ◽  
Duane Inlow ◽  
Brian L. Maiorella
Keyword(s):  
Large Scale ◽  
Perfusion Culture ◽  
High Density ◽  
Cho Cell
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