A multispecies biofilm model

1986 ◽  
Vol 28 (3) ◽  
pp. 314-328 ◽  
Author(s):  
O. Wanner ◽  
W. Gujer
Keyword(s):  
Biofilm Model ◽  
Multispecies Biofilm

A new method to solve a non-steady-state multispecies biofilm model

1993 ◽  
Vol 55 (6) ◽  
pp. 1039-1061 ◽  
Author(s):  
Vincent Gadani ◽  
Pierre Villon ◽  
Jacques Manem ◽  
Bruce Rittmann
Keyword(s):  
Steady State ◽  
New Method ◽  
Biofilm Model ◽  
Multispecies Biofilm

scholarly journals All together now: experimental multispecies biofilm model systems

2017 ◽  
Vol 19 (1) ◽  
pp. 42-53 ◽  
Author(s):  
Chuan Hao Tan ◽  
Kai Wei Kelvin Lee ◽  
Mette Burmølle ◽  
Staffan Kjelleberg ◽  
Scott A. Rice
Keyword(s):  
Model Systems ◽  
Biofilm Model ◽  
Multispecies Biofilm

scholarly journals An oral multispecies biofilm model for high content screening applications

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0173973 ◽  
Author(s):  
Nadine Kommerein ◽  
Sascha N. Stumpp ◽  
Mathias Müsken ◽  
Nina Ehlert ◽  
Andreas Winkel ◽  
...  
Keyword(s):  
High Content Screening ◽  
Biofilm Model ◽  
Multispecies Biofilm

A new method to solve a non-steady-state multispecies biofilm model

1993 ◽  
Vol 55 (6) ◽  
pp. 1039-1061 ◽  
Author(s):  
V GADANI ◽  
P VILLON ◽  
J MANEM ◽  
B RITTMANN
Keyword(s):  
Steady State ◽  
New Method ◽  
Biofilm Model ◽  
Multispecies Biofilm

Ecotoxicological effects of erythromycin on a multispecies biofilm model, revealed by metagenomic and metabolomic approaches

2021 ◽  
Vol 276 ◽  
pp. 116737
Author(s):  
Yang Pu ◽  
Jie Pan ◽  
Yuan Yao ◽  
Wing Yui Ngan ◽  
Yang Yang ◽  
...  
Keyword(s):  
Biofilm Model ◽  
Multispecies Biofilm ◽  
Ecotoxicological Effects

scholarly journals Effects of sodium citrate on the structure and microbial community composition of an early-stage multispecies biofilm model

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yuan Yao ◽  
Yang Pu ◽  
Wing Yui Ngan ◽  
Karin Kan ◽  
Jie Pan ◽  
...  
Keyword(s):  
Microbial Community ◽  
Carbon Source ◽  
Community Composition ◽  
Nutrient Medium ◽  
Sodium Citrate ◽  
Early Stage ◽  
Microbial Community Composition ◽  
Biofilm Model ◽  
Multispecies Biofilm ◽  
Biofilm Development

Abstract In recent years, most biofilm studies have focused on fundamental investigations using multispecies biofilm models developed preferentially in simulated naturally occurring low-nutrient medium than in artificial nutrient-rich medium. Because biofilm development under low-nutrient growth media is slow, natural media are often supplemented with an additional carbon source to increase the rate of biofilm formation. However, there are knowledge gaps in interpreting the effects of such supplementation on the resulting biofilm in terms of structure and microbial community composition. We investigated the effects of supplementation of a simulated freshwater medium with sodium citrate on the resulting structure, bacterial community composition, and microbial network interactions of an early-stage multispecies biofilm model. Qualitative and quantitative analyses of acquired confocal laser scanning microscopy data confirmed that sodium citrate supplementation distinctly increased biofilm biomass. Sequencing data revealed that the microbial community structure of biofilms grown in sodium citrate-supplemented conditions was characterized with increased relative abundance and dominance of Proteobacteria compared with that of biofilms grown in sodium citrate-free conditions. Our findings suggest that the supplementation of a low-nutrient medium with a carbon source in experiments involving multispecies biofilms may lead to structural and compositional biases of the microbial community, causing changes in biofilm phenotype.

scholarly journals Effects of Antimicrobial Peptide GH12 on the Cariogenic Properties and Composition of a Cariogenic Multispecies Biofilm

2018 ◽  
Vol 84 (24) ◽  
Author(s):  
Wentao Jiang ◽  
Yufei Wang ◽  
Junyuan Luo ◽  
Xinwei Li ◽  
Xuedong Zhou ◽  
...  
Keyword(s):  
Dental Caries ◽  
Antimicrobial Peptide ◽  
Streptococcus Mutans ◽  
Lactic Acid Production ◽  
Minimal Bactericidal Concentration ◽  
Content Type ◽  
Biofilm Model ◽  
Planktonic Bacteria ◽  
Multispecies Biofilm ◽  
Glucan Synthesis

ABSTRACTDental caries is a biofilm-mediated disease that occurs when acidogenic/aciduric bacteria obtain an ecological advantage over commensal species. In previous studies, the effects of the antimicrobial peptide GH12 on planktonic bacteria and monospecies biofilms were confirmed. The objectives of this study were to investigate the effects of GH12 on a cariogenic multispecies biofilm and to preliminarily explain the mechanism. In this biofilm model,Streptococcus mutansATCC 70061 was the representative of cariogenic bacteria, whileStreptococcus gordoniiATCC 35105 andStreptococcus sanguinisJCM 5708 were selected as healthy microbiota. The results showed that GH12 was more effective in suppressingS. mutansthan the other two species, with lower MIC and minimal bactericidal concentration (MBC) values among diverse type strains and clinical isolated strains. Therefore, GH12, at no more than 8 mg/liter, was used to selectively suppressS. mutansin the multispecies biofilm. GH12 at 4 mg/liter and 8 mg/liter reduced the cariogenic properties of the multispecies biofilm in biofilm formation, glucan synthesis, and lactic acid production. In addition, GH12 suppressedS. mutanswithin the multispecies biofilm and changed the bacterial composition. Furthermore, 8 mg/liter GH12 showed a selective bactericidal impact onS. mutans, and GH12 promoted hydrogen peroxide production inS. sanguinisandS. gordonii, which improved their ecological advantages. In conclusion, GH12 inhibited the cariogenic properties and changed the composition of the multispecies biofilm through a two-part mechanism by which GH12 directly suppressed the growth ofS. mutansas well as enhanced the ecological competitiveness ofS. sanguinisandS. gordonii.IMPORTANCEDental caries is one of the most prevalent chronic infectious diseases worldwide, with substantial economic and quality-of-life impacts.Streptococcus mutanshas been considered the principal pathogen of dental caries. To combat dental caries, an antimicrobial peptide, GH12, was designed, and its antibacterial effects on planktonicS. mutansand the monospecies biofilm were confirmed. As etiological concepts of dental caries evolved to include microecosystems, the homeostasis between pathogenic and commensal bacteria and a selective action on cariogenic virulence have increasingly become the focus. The novelty of this research was to study the effects of the antimicrobial peptides on a controlled cariogenic multispecies biofilm model. Notably, the role of an antimicrobial agent in regulating interspecific competition and composition shifts within this multispecies biofilm was investigated. With promising antibacterial and antibiofilm properties, the use of GH12 might be of importance in preventing and controlling caries and other dental infections.

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