scholarly journals Double-substrate-limited growth of escherichia coli

1984 ◽  
Vol 26 (11) ◽  
pp. 1398-1401 ◽  
Author(s):  
A. L. Lee ◽  
M. M. Ataai ◽  
M. L. Shuler
Keyword(s):  
Escherichia Coli ◽  
Limited Growth ◽  
Double Substrate

Design and analysis of experiments on double substrate-limited growth of Paracoccus denitrificans

1984 ◽  
Vol 50 (1) ◽  
pp. 102-102
Author(s):  
E. D. Schoen ◽  
M. A. E. van der Heijden ◽  
H. W. van Verseveld ◽  
A. H. Stouthamer ◽  
J. C. Jager
Keyword(s):  
Paracoccus Denitrificans ◽  
Limited Growth ◽  
Double Substrate

scholarly journals The lytB Gene of Escherichia coli Is Essential and Specifies a Product Needed for Isoprenoid Biosynthesis

2001 ◽  
Vol 183 (24) ◽  
pp. 7403-7407 ◽  
Author(s):  
Sean McAteer ◽  
Andrew Coulson ◽  
Neil McLennan ◽  
Millicent Masters
Keyword(s):  
Escherichia Coli ◽  
Protein Structure ◽  
Cell Lysis ◽  
Isoprenoid Biosynthesis ◽  
Globular Protein ◽  
Limited Growth ◽  
E Coli ◽  
Nonmevalonate Pathway ◽  
Tim Barrel

ABSTRACT LytB and GcpE, because they are codistributed with other pathway enzymes, have been predicted to catalyze unknown steps in the nonmevalonate pathway for isoprenoid biosynthesis. We constructed a conditional Escherichia coli lytB mutant and found that LytB is essential for survival and that depletion of LytB results in cell lysis, which is consistent with a role for this protein in isoprenoid biosynthesis. Alcohols which can be converted to pathway intermediates beyond the hypothesized LytB step(s) support limited growth of E. coli lytB mutants. An informatic analysis of protein structure suggested that GcpE is a globular protein of the TIM barrel class and that LytB is also a globular protein. Possible biochemical roles for LytB and GcpE are suggested.

Growth of Selected Cross-Contaminating Bacterial Pathogens on Beef and Fish at 15 and 35°C+

1994 ◽  
Vol 57 (4) ◽  
pp. 337-340 ◽  
Author(s):  
AJIBOLA O. FAPOHUNDA ◽  
KENNETH W. MCMILLIN ◽  
DOUGLAS L. MARSHALL ◽  
W. M. WAITES
Keyword(s):  
Escherichia Coli ◽  
Clostridium Perfringens ◽  
Aeromonas Hydrophila ◽  
Growth Response ◽  
Cross Contamination ◽  
Limited Growth ◽  
E Coli ◽  
Fish Samples ◽  
Conditioning Temperature ◽  
The Tropics

Isolates of Escherichia coli and Clostridium perfringens from beef and Aeromonas hydrophila from fish were examined for their ability to survive and grow as cross-contaminates on nonnative tissues at simulated ambient (35°C) and aging/conditioning (15°C) temperatures of handling and retailing found in the tropics. Growth of all isolates over a 10-h period was greater (P < 0.05) on their native tissues at both temperatures. The aging/conditioning temperature effectively limited growth of E. coli and A. hydrophila to less than l-logl0 CFU/g and prevented growth of C. perfringens on beef and fish samples. All three isolates demonstrated characteristic mesophilic growth response on both tissues at 35°C during the 10-h retail period. The study suggests that two muscle food products could be jointly handled to efficiently use available storage/haulage capacity in tropical countries. Potential savings in space, labor and energy would be made if cross-contamination between the two products is minimized by available packaging and sanitizing technologies.

scholarly journals Effects of sulphate-limited growth in continuous culture on the electron-transport chain and energy conservation in Escherichia coli K12

1975 ◽  
Vol 152 (3) ◽  
pp. 537-546 ◽  
Author(s):  
R K Poole ◽  
B A Haddock
Keyword(s):  
Escherichia Coli ◽  
Electron Transport ◽  
Electron Transport Chain ◽  
Functional Organization ◽  
Extracellular Polysaccharide ◽  
Limited Growth ◽  
Escherichia Coli K12 ◽  
Transport Chain ◽  
Respiratory Chains ◽  
Energy Dependent

Growth of Escherichia coli K12 in a chemostat was limited by sulphate concentrations lower than 300 muM. The synthesis of extracellular polysaccharide and a change in morphology accompanied sulphate-limited growth. Growth yields with respect to the amount of glycerol or oxygen consumed were sixfold and twofold lower respectively under these conditions than when growth was limited by glycerol. Sulphate-limited cells lacked the proton-translocating oxidoreduction segment of the electron-transport chain between NADH and the cytochromes, and particles prepared from these cells lacked the energy-dependent reduction of NAD+ by succinate, DL-α-glycerophosphate or D-lactate, suggesting the loss of site-I phosphorylation. Glycerol-limited cells contained cytochrome b556, b562 and o, ubiquinone and low concentrations of menaquinone. Sulphate limitation resulted in the additional synthesis of cytochromes d, a1, b558 and c550; the amount of ubiquinone was decreased and menaquinone was barely detectable. Non-haem iron and acid-labile sulphide concentrations were twofold lower in electron-transport particles prepared from sulphate-limited cells. Recovery of site-I phosphorylation could not be demonstrated after incubating sulphate-limited cells with or without glycerol, in either the absence or presence of added sulphate. The loss of site-I phosphorylation in sulphate-limited cells is discussed with reference to the accompanying alterations in cytochrome composition of such cells. Schemes are proposed for the functional organization of the respiratory chains of E. coli grown under conditions of glycerol or sulphate limitation.

scholarly journals Speed versus Efficiency in Microbial Growth and the Role of Parallel Pathways

2002 ◽  
Vol 184 (4) ◽  
pp. 1041-1045 ◽  
Author(s):  
Robert B. Helling
Keyword(s):  
Escherichia Coli ◽  
Glutamate Dehydrogenase ◽  
Microbial Growth ◽  
Atp Synthesis ◽  
Limited Growth ◽  
Atp Production ◽  
Parallel Pathways ◽  
Glutamate Synthesis

ABSTRACT Many microorganisms have sets of parallel pathways for ATP production in respiration and for ATP utilization in glutamate synthesis. The alternatives differ in efficiency of ATP production and utilization. The choice among these parallel pathways has been hypothesized to control the speed and efficiency of growth. Thus, the organism should be able to alleviate (or exaggerate) deficiency in one pathway by deleting another. I show here that in Escherichia coli the effect of lack of the glutamate-synthesizing enzyme glutamate dehydrogenase on glucose-limited growth is altered predictably by ndh, cyo, and cyd mutations affecting parallel pathways leading to ATP synthesis in respiration.

Sign in / Sign up

Export Citation Format

Share Document