Continuous conversion of sucrose to fructose and gluconic acid by immobilized yeast cell multienzyme complex

1980 ◽  
Vol 22 (10) ◽  
pp. 2179-2189 ◽  
Author(s):  
S. F. D'Souza ◽  
G. B. Nadkarni
Keyword(s):  
Yeast Cell ◽  
Gluconic Acid ◽  
Multienzyme Complex ◽  
Immobilized Yeast

Anaerobic Immobilized Yeast Cell Fermentation and Anaerobic Remediation in Hybrid Reactor for Mineralization of Dicarboxylic Acid Solid Waste

2005 ◽  
Vol 21 (6-7) ◽  
pp. 999-1007 ◽  
Author(s):  
Ganesh Kumar Arumugam ◽  
Sekaran Ganesan ◽  
Swarnalatha Somasundaram ◽  
Prasad Rao Burusa
Keyword(s):  
Yeast Cell ◽  
Dicarboxylic Acid ◽  
Solid Waste ◽  
Hybrid Reactor ◽  
Immobilized Yeast

Structural changes of Ca-alginate beads caused by immobilized yeast cell growth

2015 ◽  
Vol 103 ◽  
pp. 32-38 ◽  
Author(s):  
Ivana Pajic-Lijakovic ◽  
Steva Levic ◽  
Miroslav Hadnađev ◽  
Zora Stevanovic-Dajic ◽  
Radenko Radosevic ◽  
...  
Keyword(s):  
Cell Growth ◽  
Yeast Cell ◽  
Structural Changes ◽  
Alginate Beads ◽  
Immobilized Yeast ◽  
Ca Alginate

Immobilized yeast cell systems for continuous fermentation applications

2006 ◽  
Vol 28 (19) ◽  
pp. 1515-1525 ◽  
Author(s):  
Pieter J. Verbelen ◽  
David P. De Schutter ◽  
Filip Delvaux ◽  
Kevin J. Verstrepen ◽  
Freddy R. Delvaux
Keyword(s):  
Yeast Cell ◽  
Continuous Fermentation ◽  
Cell Systems ◽  
Immobilized Yeast

scholarly journals Characteristics of the Recovery of the Coenzyme A-Synthesizing Protein Complex from Saccharomyces Cerevisiae

1979 ◽  
Author(s):  
◽  
Stanley Tarnowski ◽  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Weight ◽  
Yeast Cell ◽  
Protein Complex ◽  
Coenzyme A ◽  
Pantothenic Acid ◽  
Exchange Chromatography ◽  
Yeast Cells ◽  
Multienzyme Complex ◽  
Yeast Saccharomyces Cerevisiae

A multienzyme complex contained in Bakers' yeast (Saccharomyces cerevisiae) which synthesizes CoA has been named the coenzyme A-synthesizing protein complex (CoA-SPC). The CoA-SPC has been shown to be insoluble in the crude Bakers' yeast cell lysate formed by exposing the yeast cell to ether and dry ice. Only after solubilization has this multienzyme complex been shown to catalyze the formation of bound dephospho-CoA utilizing the substrates adenosine triphosphate, D-pantothenic acid and L-cysteine. A low molecular weight component or components of the soluble fraction of the yeast cell and chloride ion appears to be responsible for the solubilization of CoA-SPC. This endogenous component of the yeast cell is referred to as t-Factor. Purification of t-Factor has been accomplished by techniques which have included dialysis, ultrafiltration and paper, permeation, adsorption and ion-exchange chromatography. Although the t-Factor has not been identified, several properties have been demonstrated: (1) the molecular weight is between 400 to 1,000; (2) it is stable to heat at 80°C for 24 hours; (3) it is resistant to hydrolysis by trypsin and protease; (4) it is inactivated by ashing; and (5) has no ultraviolet absorption at 260 mu and 280 my. Replacement of t-Factor by commercially available compounds or solubilizing agents has failed to reveal its identity. A "one-step" purification of CoA-SPC may be obtained by combining partially purified t-Factor with insoluble yeast cell residue. An alternate procedure was developed for the preparation of CoA-SPC. This procedure involves the slow drying and rehydration of the yeast cells, but the need for t-Factor still remains. Sonic oscillation and passage through a french pressure cell, two of the more classical cell breakage techniques, fail to produce active CoA-SPC preparations.

scholarly journals Continuous Beer Fermentation Using Immobilized Yeast Cell Bioreactor Systems

2008 ◽  
Vol 21 (3) ◽  
pp. 653-663 ◽  
Author(s):  
Tomáš Brányik ◽  
António A. Vicente ◽  
Pavel Dostálek ◽  
José A. Teixeira
Keyword(s):  
Yeast Cell ◽  
Immobilized Yeast ◽  
Beer Fermentation

scholarly journals Tomography of a Cryo-immobilized Yeast Cell Using Ptychographic Coherent X-Ray Diffractive Imaging

2015 ◽  
Vol 109 (9) ◽  
pp. 1986-1995 ◽  
Author(s):  
K. Giewekemeyer ◽  
C. Hackenberg ◽  
A. Aquila ◽  
R.N. Wilke ◽  
M.R. Groves ◽  
...  
Keyword(s):  
Yeast Cell ◽  
Diffractive Imaging ◽  
Immobilized Yeast ◽  
X Ray
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