Isolation of a yeast-lyzingArthrobacter species and the production of the lytic enzyme complex in batch and continuous-flow fermentors

1977 ◽  
Vol 19 (6) ◽  
pp. 879-899 ◽  
Author(s):  
B. I. Rowley ◽  
A. T. Bull
Keyword(s):  
Continuous Flow ◽  
Lytic Enzyme ◽  
Enzyme Complex

Antifungal action of the lytic enzyme complex from Lysobacter sp. XL1

2005 ◽  
Vol 40 (2) ◽  
pp. 557-564 ◽  
Author(s):  
L.P. Ryazanova ◽  
O.A. Stepnaya ◽  
N.E. Suzina ◽  
I.S. Kulaev
Keyword(s):  
Lytic Enzyme ◽  
Enzyme Complex ◽  
Antifungal Action

Autolysis of Penicillium oxalicum with special reference to its cell walls

1982 ◽  
Vol 28 (12) ◽  
pp. 1289-1295 ◽  
Author(s):  
M. I. Perez-Leblic ◽  
Fuensanta Reyes ◽  
R. Lahoz ◽  
S. A. Archer
Keyword(s):  
Cell Walls ◽  
Schizophyllum Commune ◽  
Dry Weight ◽  
Penicillium Oxalicum ◽  
Lytic Enzyme ◽  
Enzyme Complex ◽  
Fungal Cell ◽  
Rapid Hydrolysis ◽  
And Storage ◽  
Hydrolysis Of

Cultures of Penicillium oxalicum growing on a denned medium supplemented with yeast extract reached the onset of autolysis after 3 days at 25 °C. Thenceforth, autolysis was progressive and eventual reductions in dry weight of 96% were recorded by day 47. The pH of the medium fluctuated between 4.0 during the exponential phase of growth and 9.0 during autolysis. Electron microscopy of autolyzing cultures revealed a progressive loss of cytoplasmic ultrastructure. Digestion of the cell walls, with a rapid hydrolysis of the three external layers and a low hydrolysis of the two inner layers, was accompanied by deep pitting and by loss of the distinct five-layered structure. A lytic enzyme complex was obtained from the filtrates of extensively autolyzed cultures. It was rich in (1 → 3)-β-glucanase and other enzymes active against a range of fungal cell wall and storage polysaccharides. This enzyme complex degraded extensively isolated cell walls of P. oxalicum and three other Ascomycetes but had less effect on walls isolated from Mucor mucedo or Schizophyllum commune. In the case of P. oxalicum, cell walls harvested from young cultures were more readily digested than were the walls from older cultures.

"PROTOPLASTS" FROM PYTHIUM SP. PRL 2142

1967 ◽  
Vol 13 (12) ◽  
pp. 1701-1704 ◽  
Author(s):  
J. H. Sietsma ◽  
D. E. Eveleigh ◽  
R. H. Haskins ◽  
J. F. T. Spencer
Keyword(s):  
Lytic Enzyme ◽  
Enzyme Complex ◽  
Enzyme Preparations ◽  
Streptomyces Spp ◽  
Osmotic Stabilizer ◽  
Enzyme Complexes

Enzyme preparations from two Streptomyces spp. released spherical protoplast-like bodies from the mycelium of Pythium sp. PRL 2142. The enzyme complexes were produced by growing the Streptomyces spp. in the presence of autoclaved Pythium mycelium, and concentrated from the culture mixture by precipitation with acetone (2:1, v/v). The protoplasts were released, from both intercalary and terminal locations on the hyphae, during incubation with the lytic enzyme complex for 24 hours using 0.8 M MgSO4 as an osmotic stabilizer.

scholarly journals Multiple β-1, 3 Glucanases in the Lytic Enzyme Complex of Bacillus circulans WL 12

1974 ◽  
Vol 38 (5) ◽  
pp. 959-965 ◽  
Author(s):  
Yoshinori KOBAYASHI ◽  
Hirosato TANAKA ◽  
Nagahiro OGASAWARA
Keyword(s):  
Bacillus Circulans ◽  
Lytic Enzyme ◽  
Enzyme Complex

Multipleβ-1,3 Glucanases in the Lytic Enzyme Complex ofBacillus circulansWL 12

1974 ◽  
Vol 38 (5) ◽  
pp. 959-965
Author(s):  
Yoshinori Kobayashi ◽  
Hirosato Tanaka ◽  
Nagahiro Ogasawara
Keyword(s):  
Lytic Enzyme ◽  
Enzyme Complex

Lytic enzyme complex of an antagonistic Bacillus sp. X-b: isolation and purification of components

2001 ◽  
Vol 758 (2) ◽  
pp. 197-205 ◽  
Author(s):  
Pirkko Helistö ◽  
Gleb Aktuganov ◽  
Nailia Galimzianova ◽  
Alexander Melentjev ◽  
Timo Korpela
Keyword(s):  
Lytic Enzyme ◽  
Enzyme Complex ◽  
Bacillus Sp ◽  
Isolation And Purification

Catalyst- and oxidant-free electrochemical para-selective hydroxylation of N-arylamides in batch and continuous-flow

2020 ◽  
Vol 22 (19) ◽  
pp. 6437-6443
Author(s):  
Cheng-Kou Liu ◽  
Meng-Yi Chen ◽  
Xin-Xin Lin ◽  
Zheng Fang ◽  
Kai Guo
Keyword(s):  
Ammonium Salt ◽  
Quaternary Ammonium ◽  
Continuous Flow ◽  
Quaternary Ammonium Salt ◽  
Selective Hydroxylation

A catalyst-, oxidant-, acidic solvent- and quaternary ammonium salt-free electrochemical para-selective hydroxylation of N-arylamides at rt in batch and continuous-flow was developed.

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