A novel strategy for selective gene delivery by using the inhibitory effect of blue light on jetPRIME-mediated transfection

2015 ◽  
Vol 113 (7) ◽  
pp. 1560-1567
Author(s):  
Minori Dateki ◽  
Osamu Imamura ◽  
Masaaki Arai ◽  
Hidehisa Shimizu ◽  
Kunio Takishima
Keyword(s):  
Gene Delivery ◽  
Blue Light ◽  
Inhibitory Effect ◽  
Novel Strategy

scholarly journals Diminution of feeding response in Helicoverpa armigera by inhibition or silencing of sugar gustatory receptor

2019 ◽  
Author(s):  
Aniruddha R. Agnihotri ◽  
Sanyami S. Zunjarrao ◽  
Mukta Nagare ◽  
Rakesh S. Joshi
Keyword(s):  
Helicoverpa Armigera ◽  
Feeding Rate ◽  
Receptor Activation ◽  
Inhibitory Effect ◽  
Gustatory Receptor ◽  
Feeding Response ◽  
Downstream Signaling ◽  
Helicoverpa Armígera ◽  
Novel Strategy

ABSTRACTGustatory receptor (GR) is one of the essential chemosensory molecules in Lepidopteran pests. GR is involved in sensing several canonical tastes which in turn regulate the diverse behavioral and physiological responses of these insects. In this article, we have evaluated the alteration in feeding response of Helicoverpa armigera by blocking and silencing of sugar-sensing gustatory receptor 9 (HarmGr9). Sf9 cells based assay showed that glucose analogue, Miglitol, can bind to the expressed HarmGr9. This binding might lead to an inhibition of receptor activation and downstream signaling, indicated by reduced intracellular Ca2+ fluorescence. Further, the in-vivo study illustrated the feeding rate reduced on a diet containing miglitol as compared to the larvae fed on the artificial diet. Reduction in feeding rate was prolonged in insects fed on the miglitol containing diet even after switching to the control glucose diet. Competitive cell-based assay and feeding assay, using equimolar glucose and miglitol, also showed an inhibitory effect on HarmGr-9 activation and insect feeding rate. We have observed similar feeding rate reduction in HarmGr9 knockdown in H. armigera larvae. We believe this unique approach of H. armigera feeding response inhibition by blocking the sugar receptor can be further used to develop a novel strategy for agricultural pest management.

Systemic hypoxia potentiates anti-tumor effects of metformin in hepatocellular carcinoma in mice

2020 ◽  
Vol 52 (4) ◽  
pp. 421-429
Author(s):  
Hui Lin ◽  
Wenfang Zhou ◽  
Yonghong Huang ◽  
Min Ren ◽  
Fangyun Xu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Tumor Progression ◽  
Tumor Volume ◽  
Combined Treatment ◽  
Hypoxia Inducible Factor ◽  
Inhibitory Effect ◽  
Whole Body ◽  
Matrix Metalloproteinase 2 ◽  
Systemic Hypoxia ◽  
Novel Strategy

Abstract Local hypoxia is a universal phenomenon in most solid tumors. The role of local hypoxia in the tumor microenvironment and cancer growth and metastasis has been well established. However, the effect of acute systemic hypoxia (exposing the whole body to 10% O2 environment) on cancer has not yet been investigated. In this study, we investigated the potential effects of acute systemic hypoxia itself and in combination with metformin on hepatocellular carcinoma (HCC) growth and metastasis in a mouse model of HCC. Acute systemic hypoxia significantly decreased tumor volume and weight in H22 tumor-bearing mice. Interestingly, the combined treatment of acute systemic hypoxia and metformin showed a more pronounced effect in reducing tumor volume and weight. Moreover, acute systemic hypoxia and metformin in combination had a potent inhibitory effect on tumor progression. More importantly, the expressions of hypoxia response genes including hypoxia-inducible factor-1 α, vascular endothelial growth factor, and matrix metalloproteinase 2 were significantly decreased in the tumor tissues with combination treatment. Our study demonstrated that acute systemic hypoxia repressed tumor progression of the HCC and potentiated the anti-tumor activities of metformin. This study supports that combination of systemic hypoxia and metformin treatment may represent a novel strategy for HCC.

scholarly journals Cysteine: an overlooked energy and carbon source

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Luise Göbbels ◽  
Anja Poehlein ◽  
Albert Dumnitch ◽  
Richard Egelkamp ◽  
Cathrin Kröger ◽  
...  
Keyword(s):  
Carbon Source ◽  
Blue Light ◽  
Metabolic Activity ◽  
Cadmium Sulphide ◽  
Inhibitory Effect ◽  
Arginine Metabolism ◽  
Moorella Thermoacetica ◽  
Acetogenic Bacterium ◽  
Intracellular Storage ◽  
Biological Component

AbstractBiohybrids composed of microorganisms and nanoparticles have emerged as potential systems for bioenergy and high-value compound production from CO2 and light energy, yet the cellular and metabolic processes within the biological component of this system are still elusive. Here we dissect the biohybrid composed of the anaerobic acetogenic bacterium Moorella thermoacetica and cadmium sulphide nanoparticles (CdS) in terms of physiology, metabolism, enzymatics and transcriptomic profiling. Our analyses show that while the organism does not grow on l-cysteine, it is metabolized to acetate in the biohybrid system and this metabolism is independent of CdS or light. CdS cells have higher metabolic activity, despite an inhibitory effect of Cd2+ on key enzymes, because of an intracellular storage compound linked to arginine metabolism. We identify different routes how cysteine and its oxidized form can be innately metabolized by the model acetogen and what intracellular mechanisms are triggered by cysteine, cadmium or blue light.

scholarly journals In Arabidopsis, low blue light enhances phototropism by releasing cryptochrome 1-mediated inhibition of PIF4 expression

2020 ◽  
Author(s):  
Alessandra Boccaccini ◽  
Martina Legris ◽  
Johanna Krahmer ◽  
Laure Allenbach-Petrolati ◽  
Anupama Goyal ◽  
...  
Keyword(s):  
Blue Light ◽  
Solar Spectrum ◽  
Inhibitory Effect ◽  
Phytochrome B ◽  
Growth Responses ◽  
Swiss National Science Foundation ◽  
Etiolated Seedlings ◽  
The University ◽  
Cryptochrome 1 ◽  
Favorable Light

AbstractShade-avoiding plants including Arabidopsis thaliana display a number of growth responses elicited by shade cues including elongation of stem-like structures and repositioning of leaves. Shade also promotes phototropism of de-etiolated seedlings through repression of phytochrome B (phyB) presumably to enhance capture of unfiltered sunlight. Light cues indicative of shade include a reduction in the blue and red portions of the solar spectrum and a low red to far-red ratio. Here we show that in Arabidopsis seedlings both low blue and a low red to far-red ratio are required to rapidly enhance phototropism. However, prolonged low blue treatments through reduced cryptochrome 1 (cry1) activation are sufficient to promote phototropism. The enhanced phototropic response of cry1 mutants in the lab and in response to natural canopies depends on PHYTOCHROME INTERACTING FACTORs (PIFs). In favorable light conditions, cry1 limits the expression of PIF4 while in low blue light PIF4 expression increases, which contributes to phototropic enhancement. The analysis of a quantitative DII auxin reporter indicates that low blue light leads to enhanced auxin levels in the hypocotyl and, upon phototropic stimulation, a steeper auxin gradient across the hypocotyl. We conclude that phototropic enhancement by canopy shade results from the combined activities of phytochrome B and cry1 that converge on PIF regulation.ONE SENTENCE SUMMARYThe persistent depletion of blue light in natural canopy shade relieves the inhibitory effect of cryptochrome 1 on PIF4, enhancing phototropism in de-etiolated Arabidopsis seedlings.Financial supportThis work was supported by the University of Lausanne and a grant from the Swiss National Science foundation (n° 310030B_179558 to C.F.); Human Frontier Science Program organization (HFSP) Grant RPG0054-2013, ANR-12-BSV6-0005 grant (AuxiFlo) to T.V.; The University of Buenos Aires (Grant 20020100100437 to J. J. C.), and Agencia Nacional de Promoción Científica y Tecnológica of Argentina (Grant PICT-2018-01695 to J. J. C.). Alessandra Boccaccini and Martina Legris are funded by Marie Curie fellowships H2020-MSCA-IF-2017 grants CRoSh 796283 and Flat-Leaf 796443 respectively.

The Development of Phototaxis in the Frog (Rana Temporaria)

1963 ◽  
Vol 40 (2) ◽  
pp. 371-379
Author(s):  
W. R. A. MUNTZ
Keyword(s):  
Blue Light ◽  
Rana Temporaria ◽  
Green Light ◽  
Inhibitory Effect ◽  
Synergistic Action ◽  
Excitatory Effect ◽  
Frog Rana Temporaria ◽  
Phototactic Behaviour

1. The development of phototaxis in the frog was studied by testing the behaviour of tadpoles at different stages of their development. The development of the retina at each stage was checked from histological preparations. 2. Young tadpoles responded most strongly to green light (approx. 550 mµ). Gradually the number of responses to green light decreased, while more responses were made to blue light, and also to darkness. Adult frogs respond most strongly to blue light; light of wavelengths longer than about 510 mµ is responded to only slightly more often than darkness. 3. It was concluded that the phototactic behaviour of frogs is probably mediated by a synergistic action of the green rods and the cones, which have an excitatory effect, and which are opposed by an inhibitory effect of the red rods.

Novel Strategy of Gene Delivery System Based on Dendrimer Loaded Recombinant Hirudine Plasmid for Thrombus Targeting Therapy

2019 ◽  
Vol 16 (4) ◽  
pp. 1648-1657 ◽  
Author(s):  
Junjie Chen ◽  
Yanping Lu ◽  
Ying Cheng ◽  
Rui Ma ◽  
Jiafeng Zou ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Gene Delivery System ◽  
Targeting Therapy ◽  
Novel Strategy

A novel strategy of selective gene delivery by using a uniform magnetic field

2020 ◽  
pp. 2000233
Author(s):  
Minori Dateki ◽  
Osamu Imamura ◽  
Masaaki Arai ◽  
Hidehisa Shimizu ◽  
Kunio Takishima
Keyword(s):  
Magnetic Field ◽  
Gene Delivery ◽  
Uniform Magnetic Field ◽  
Novel Strategy

scholarly journals TGR5 Activation Modulates an Inhibitory Effect on Liver Fibrosis Development Mediated by Anagliptin in Diabetic Rats

Cells ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 1153 ◽  
Author(s):  
Daisuke Kaya ◽  
Kosuke Kaji ◽  
Yuki Tsuji ◽  
Satoko Yamashita ◽  
Koh Kitagawa ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Inhibitory Effect ◽  
Diabetic Rats ◽  
Proliferative Potential ◽  
Fecal Microbiome ◽  
Dipeptidyl Peptidase 4 ◽  
Porcine Serum ◽  
Novel Strategy ◽  
G Protein Coupled

Hyperglycemia and hyperinsulinemia activate the proliferative potential of hepatic stellate cells (HSCs) and promote hepatic fibrosis. Dipeptidyl peptidase-4 (DPP-4) inhibitors, antidiabetic agents, reportedly inhibit the HSC proliferation. Additionally, Takeda G protein-coupled receptor 5 (TGR5) agonists induce the systemic release of glucagon-like peptides from intestinal L cells, which maintains glycemic homeostasis. This study assessed the combined effect of TGR5 agonist and DPP-4 inhibitor on diabetes-based liver fibrosis development. Male diabetic rats received intraperitoneal injection of porcine serum (PS) to induce liver fibrosis, and they were orally administered the following agents: oleanolic acid (OA) as a TGR5 agonist, anagliptin (ANA) as a DPP-4 inhibitor, and a combination of both agents. Treatment with OA or ANA significantly improved glycemic status and attenuated intrahepatic steatosis and lipid peroxidation in diabetic rats. PS-induced liver fibrosis development was also drastically suppressed by treatment with either agent, and the combination of both reciprocally enhanced the antifibrotic effect. Fecal microbiome demonstrated that both agents inhibited the increase in the Firmicutes/Bacteroidetes ratio, an indicator of dysbiosis related to metabolic syndromes. Furthermore, ANA directly inhibited in vitro HSC proliferative and profibrogenic activities. Collectively, TGR5 agonist and DPP-4 inhibitor appears to be a novel strategy against liver fibrosis under diabetic conditions.

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