Rewiring a carbohydrate oxidase

2015 ◽  
Vol 112 (6) ◽  
pp. vi-vi
Keyword(s):  
Carbohydrate Oxidase

The development of an improved glucose biosensor using recombinant carbohydrate oxidase from Microdochium nivale

The Analyst ◽  
2000 ◽  
Vol 125 (9) ◽  
pp. 1587-1590 ◽  
Author(s):  
Juozas Kulys ◽  
Lidija Tetianec ◽  
Palle Schneider
Keyword(s):  
Glucose Biosensor ◽  
Microdochium Nivale ◽  
Carbohydrate Oxidase

Carbohydrate oxidase, a novel enzyme from polyporus obtusus

1968 ◽  
Vol 167 (3) ◽  
pp. 493-500 ◽  
Author(s):  
Hans W. Ruelius ◽  
Richard M. Kerwin ◽  
Frank W. Janssen
Keyword(s):  
Carbohydrate Oxidase

scholarly journals Cloning of the gene for a carbohydrate oxidase from Lactuca sativa in the yeasts Saccharomyces cerevisiae and Pichia pastoris

2015 ◽  
Vol 69 (6) ◽  
pp. 689-701
Author(s):  
Vojin Tadic ◽  
Ana Balaz ◽  
Marija Petric ◽  
Snezana Milosevic ◽  
Nevena Zelenovic ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Pichia Pastoris ◽  
Lactuca Sativa ◽  
Gene Sequence ◽  
Extracellular Expression ◽  
Empty Vector ◽  
Two Samples ◽  
Foreign Genes ◽  
Carbohydrate Oxidase

We have cloned the gene for carbohydrate oxidase (CHO) from Lactuca sativa in two species of yeasts (Saccharomyces cerevisiae and Pichia pastoris). The synthetic gene for the carbohydrate oxidase (1821 bp) from L. sativa cloned into the vector pUC57 and inserted into plasmids pYES2 and pGAP using Escherichia coli DH5? strain. The P. pastoris strain X-33 and the S. cerevisiae strain InvSC1 were used for extracellular expression of CHO. After transformation of P. pastoris X-33 with CHO-pGAP construct none of the colonies showed CHO activity. Two samples displayed a band which did not exist in the sample with the empty vector similar to the molecular weight of CHO. The S. cerevisiae strain InvSC1 has been also transformed with CHO-pYES constructs. Three colonies grew on the plate with cells transformed with the construct. One of the samples showed a band corresponding to about 110 kDa, but no CHO activity was recorded in this case either. Cloning of the foreign genes and heterologous expression in yeasts is widely used in biotechnology, but sometimes can be very dependent on the gene sequence and strain used. In order to obtain active CHO enzyme further studies on purification and refolding of expressed protein are necessary.

Carbohydrate oxidase, a novel enzyme from polyporus obtusus

1968 ◽  
Vol 167 (3) ◽  
pp. 501-510 ◽  
Author(s):  
Frank W. Janssen ◽  
Hans W. Ruelius
Keyword(s):  
Carbohydrate Oxidase

scholarly journals [Review] Development of Sour Syrup “Oligo-Sugar Acid” Based on Maltobionic Acid Using Carbohydrate Oxidase

2020 ◽  
Vol 10 (1) ◽  
pp. 36-41
Author(s):  
Ken Fukami ◽  
Daiki Suehiro ◽  
Kanako Hayashi ◽  
Masamichi Okada ◽  
Akio Horii ◽  
...  
Keyword(s):  
Carbohydrate Oxidase

scholarly journals A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family

PLoS ONE ◽  
2019 ◽  
Vol 14 (5) ◽  
pp. e0216546 ◽  
Author(s):  
Filip Mollerup ◽  
Ville Aumala ◽  
Kirsti Parikka ◽  
Yann Mathieu ◽  
Harry Brumer ◽  
...  
Keyword(s):  
Carbohydrate Oxidase

scholarly journals CARBOHYDRATE OXIDASE FROM A RED ALGA, IRIDOPHYCUS FLACCIDUM

1956 ◽  
Vol 218 (1) ◽  
pp. 425-436
Author(s):  
R.C. Bean ◽  
W.Z. Hassid
Keyword(s):  
Red Alga ◽  
Carbohydrate Oxidase
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