The development of an improved glucose biosensor using recombinant carbohydrate oxidase from Microdochium nivale

The Analyst ◽  
2000 ◽  
Vol 125 (9) ◽  
pp. 1587-1590 ◽  
Author(s):  
Juozas Kulys ◽  
Lidija Tetianec ◽  
Palle Schneider
Keyword(s):  
Glucose Biosensor ◽  
Microdochium Nivale ◽  
Carbohydrate Oxidase

Organic-Inorganic Hybrid Ultra-Thin Films Applied to Glucose Biosensor

2011 ◽  
Vol E94-C (12) ◽  
pp. 1855-1857
Author(s):  
Huihui WANG ◽  
Hitoshi OHNUKI ◽  
Hideaki ENDO ◽  
Mitsuru IZUMI
Keyword(s):  
Thin Films ◽  
Glucose Biosensor ◽  
Inorganic Hybrid

Detection of Glucose in Human Serum Based on Silicon Dot Probe

2020 ◽  
Vol 16 (6) ◽  
pp. 744-752
Author(s):  
Kuan Luo ◽  
Xinyu Jiang
Keyword(s):  
Quantum Dots ◽  
Blood Glucose ◽  
Human Serum ◽  
Organic Dyes ◽  
Limit Of Detection ◽  
Fasting Blood Glucose ◽  
Glucose Biosensor ◽  
Metal Nanoclusters ◽  
Glucose Levels ◽  
Highly Sensitive

Background: Diabetes Mellitus (DM) is a major public metabolic disease that influences 366 million people in the world in 2011, and this number is predicted to rise to 552 million in 2030. DM is clinically diagnosed by a fasting blood glucose that is equal or greater than 7 mM. Therefore, the development of effective glucose biosensor has attracted extensive attention worldwide. Fluorescence- based strategies have sparked tremendous interest due to their rapid response, facile operation, and excellent sensitivity. Many fluorescent compounds have been employed for precise analysis of glucose, including quantum dots, noble metal nanoclusters, up-converting nanoparticles, organic dyes, and composite fluorescent microspheres. Silicon dot as promising quantum dots materials have received extensive attention, owing to their distinct advantages such as biocompatibility, low toxicity and high photostability. Methods: MnO2 nanosheets on the Si nanoparticles (NPs) surface serve as a quencher. Si NPs fluorescence can make a recovery by the addition of H2O2, which can reduce MnO2 to Mn2+, and the glucose can thus be monitored based on the enzymatic conversion of glucose by glucose oxidase to generate H2O2. Therefore, the glucose concentration can be derived by recording the fluorescence recovery spectra of the Si NPs. Results: This probe enabled selective detection of glucose with a linear range of 1-100 μg/mL and a limit of detection of 0.98 μg/mL. Compared with the commercial glucometer, this method showed favorable results and convincing reliability. Conclusion: We have developed a novel method based on MnO2 -nanosheet-modified Si NPs for rapid monitoring of blood glucose levels. By combining the highly sensitive H2O2/MnO2 reaction with the excellent photostability of Si NPs, a highly sensitive, selective, and cost-efficient sensing approach for glucose detection has been designed and applied to monitor glucose levels in human serum with satisfactory results.

Temperature-induced amperometric glucose biosensor based on a poly(N-vinylcaprolactam)/graphene oxide composite film

The Analyst ◽  
2019 ◽  
Vol 144 (6) ◽  
pp. 1960-1967 ◽  
Author(s):  
Chao Chen ◽  
Pengcheng Zhao ◽  
Meijun Ni ◽  
Chunyan Li ◽  
Yixi Xie ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Composite Film ◽  
Glucose Oxidase ◽  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Glucose Biosensor ◽  
Carbon Electrode ◽  
Oxide Composite

A temperature-induced sensing film consisting of poly(N-vinylcaprolactam) (PVCL), graphene oxide (GO) and glucose oxidase (GOD) was fabricated and used to modify a glassy carbon electrode (GCE).

Flexible electrochemical uric acid and glucose biosensor

2021 ◽  
pp. 107870
Author(s):  
Md Faruk Hossain ◽  
Gymama Slaughter
Keyword(s):  
Uric Acid ◽  
Glucose Biosensor
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