Enhanced recovery of antitumor ganoderic acid T from Ganoderma lucidum mycelia by novel chemical conversion strategy

2011 ◽  
Vol 109 (3) ◽  
pp. 754-762 ◽  
Author(s):  
Jia-Le Wang ◽  
Tingyue Gu ◽  
Jian-Jiang Zhong
Keyword(s):  
Ganoderma Lucidum ◽  
Enhanced Recovery ◽  
Chemical Conversion ◽  
Ganoderic Acid

scholarly journals Optimization of Culture Condition for Ganoderic Acid Production in Ganoderma lucidum Liquid Static Culture and Design of a Suitable Bioreactor

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2563 ◽  
Author(s):  
Gaosheng Hu ◽  
Manhuayun Zhai ◽  
Rong Niu ◽  
Xiaoqiang Xu ◽  
Qian Liu ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Liquid Culture ◽  
Management Practices ◽  
Total Yield ◽  
Gas Content ◽  
Market Demand ◽  
Ganoderic Acid ◽  
Static Culture ◽  
Air Supply ◽  
Liquid Static Culture

Ganoderma lucidum, a famous medicinal mushroom used worldwide, is a rich source of triterpenoids which, together with polysaccharides, are believed to be the main effective constituents of G. lucidum. With the increase of market demand, the wild resource is facing serious limitations, and the quality of cultivated fruiting bodies can be seriously affected by the availability of wood resources and by cultivation management practices. In the present study, we aimed to develop an alternative way to produce useful triterpenoids from G. lucidum. We cultured the strain using a two-stage liquid culture strategy and investigated the effects of nitrogen limitation, carbon supply, static culture volume and air supply in the static culture stage on the accumulation of five triterpenoids (GA-P, GA-Q, GA-T, GA-S, GA-R). Our results showed that, under optimized condition, the total yield of the five triterpenoids reached 963 mg/L (as determined by HPLC). Among the five triterpenoids, GA-T accounted for about 75% of the total yield. Besides, a bioreactor suitable for fungal liquid static culture with a 10 L extensible plastic bag shaped culture unit was designed and in which the maximum total yield of the five GAs reached 856.8 mg/L, and the GAs content reached 5.99%. Our results demonstrate the potential of industrial application of G. lucidum culture for the production of triterpenoids, especially GA-T. Air supply significantly improved the accumulation of triterpenoids, and this will provide important clues to understand why more triterpenoids are produced in the mycelia mat under static liquid culture conditions.

A new epoxidic ganoderic acid and other phytoconstituents from Ganoderma lucidum

2011 ◽  
Vol 4 (3) ◽  
pp. 386-388 ◽  
Author(s):  
Soniamol Joseph ◽  
Kainoor K. Janardhanan ◽  
Varughese George ◽  
Sabulal Baby
Keyword(s):  
Ganoderma Lucidum ◽  
Ganoderic Acid

scholarly journals Alternative oxidase impacts ganoderic acid biosynthesis by regulating intracellular ROS levels in Ganoderma lucidum

Microbiology ◽  
2017 ◽  
Vol 163 (10) ◽  
pp. 1466-1476 ◽  
Author(s):  
Deng-Ke Shi ◽  
Jing Zhu ◽  
Ze-Hua Sun ◽  
Guang Zhang ◽  
Rui Liu ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Alternative Oxidase ◽  
Acid Biosynthesis ◽  
Ganoderic Acid ◽  
Intracellular Ros

scholarly journals Enhancement of Ganoderic Acid Accumulation by Overexpression of an N-Terminally Truncated 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase Gene in the Basidiomycete Ganoderma lucidum

2012 ◽  
Vol 78 (22) ◽  
pp. 7968-7976 ◽  
Author(s):  
Jun-Wei Xu ◽  
Yi-Ning Xu ◽  
Jian-Jiang Zhong
Keyword(s):  
Ganoderma Lucidum ◽  
Coenzyme A ◽  
Catalytic Domain ◽  
Selection Marker ◽  
Transformation System ◽  
Protein Subunit ◽  
Ganoderic Acid ◽  
Content Type ◽  
Ganoderic Acids ◽  
Coenzyme A Reductase

ABSTRACTGanoderic acids produced byGanoderma lucidum, a well-known traditional Chinese medicinal mushroom, exhibit antitumor and antimetastasis activities. Genetic modification ofG. lucidumis difficult but critical for the enhancement of cellular accumulation of ganoderic acids. In this study, a homologous genetic transformation system forG. lucidumwas developed for the first time using mutatedsdhB, encoding the iron-sulfur protein subunit of succinate dehydrogenase, as a selection marker. The truncatedG. lucidumgene encoding the catalytic domain of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) was overexpressed by using theAgrobacterium tumefaciens-mediated transformation system. The results showed that the mutatedsdhBsuccessfully conferred carboxin resistance upon transformation. Most of the integrated transfer DNA (T-DNA) appeared as a single copy in the genome. Moreover, deregulated constitutive overexpression of the HMGR gene led to a 2-fold increase in ganoderic acid content. It also increased the accumulation of intermediates (squalene and lanosterol) and the upregulation of downstream genes such as those of farnesyl pyrophosphate synthase, squalene synthase, and lanosterol synthase. This study demonstrates that transgenic basidiomyceteG. lucidumis a promising system to achieve metabolic engineering of the ganoderic acid pathway.

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