scholarly journals Alternative oxidase impacts ganoderic acid biosynthesis by regulating intracellular ROS levels in Ganoderma lucidum

Microbiology ◽  
2017 ◽  
Vol 163 (10) ◽  
pp. 1466-1476 ◽  
Author(s):  
Deng-Ke Shi ◽  
Jing Zhu ◽  
Ze-Hua Sun ◽  
Guang Zhang ◽  
Rui Liu ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Alternative Oxidase ◽  
Acid Biosynthesis ◽  
Ganoderic Acid ◽  
Intracellular Ros

Nitrate reductase-dependent nitric oxide plays a key role on MeJA-induced ganoderic acid biosynthesis in Ganoderma lucidum

2020 ◽  
Vol 104 (24) ◽  
pp. 10737-10753
Author(s):  
Liang Shi ◽  
Sining Yue ◽  
Tan Gao ◽  
Jing Zhu ◽  
Ang Ren ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitrate Reductase ◽  
Ganoderma Lucidum ◽  
Acid Biosynthesis ◽  
Ganoderic Acid

Functions of reactive oxygen species in apoptosis and ganoderic acid biosynthesis in Ganoderma lucidum

2019 ◽  
Vol 366 (23) ◽  
Author(s):  
Jing Zhu ◽  
Fengli Wu ◽  
Sining Yue ◽  
Chen Chen ◽  
Shuqi Song ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Ganoderma Lucidum ◽  
Reactive Oxygen ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Activity Levels ◽  
Oxygen Species ◽  
Ganoderic Acid ◽  
Medicinal Fungus ◽  
Intracellular Ros ◽  
Ga Biosynthesis

ABSTRACT Ganoderma lucidum is a medicinal fungus that is widely used in traditional medicine. Fungal PacC is recognized as an important transcription factor that functions during adaptation to environmental pH, fungal development and secondary metabolism. Previous studies have revealed that GlPacC plays important roles in mycelial growth, fruiting body development and ganoderic acid (GA) biosynthesis. In this study, using a terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) assay, we found that the apoptosis level was increased when PacC was silenced. The transcript and activity levels of caspase-like proteins were significantly increased in the PacC-silenced (PacCi) strains compared with the control strains. Silencing PacC also resulted in an increased reactive oxygen species (ROS) levels (∼2-fold) and decreased activity levels of enzymes involved in the antioxidant system. Further, we found that the intracellular ROS levels contributed to apoptosis and GA biosynthesis. Adding N-acetyl-cysteine and vitamin C decreased intracellular ROS and resulted in the inhibition of apoptosis in the PacCi strains. Additionally, the GA biosynthesis was different between the control strains and the PacCi strains after intracellular ROS was eliminated. Taken together, the findings showed that silencing PacC can result in an intracellular ROS burst, which increases cell apoptosis and GA biosynthesis levels. Our study provides novel insight into the functions of PacC in filamentous fungi.

Ethylene promotes mycelial growth and ganoderic acid biosynthesis in Ganoderma lucidum

2016 ◽  
Vol 39 (2) ◽  
pp. 269-275 ◽  
Author(s):  
Guang Zhang ◽  
Ang Ren ◽  
Fengli Wu ◽  
Hanshou Yu ◽  
Liang Shi ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Mycelial Growth ◽  
Acid Biosynthesis ◽  
Ganoderic Acid

scholarly journals Induction of apoptosis and ganoderic acid biosynthesis by cAMP signaling in Ganoderma lucidum

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Bang-Jau You ◽  
Ni Tien ◽  
Miin-Huey Lee ◽  
Bo-Ying Bao ◽  
Yih-Shyuan Wu ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Camp Signaling ◽  
Acid Biosynthesis ◽  
Ganoderic Acid ◽  
Induction Of Apoptosis

Function of ceramide synthases on growth, ganoderic acid biosynthesis and sphingolipid homeostasis in Ganoderma lucidum

2020 ◽  
Vol 172 ◽  
pp. 112283
Author(s):  
Xiaoxiao Lu ◽  
Chunqin Xie ◽  
Yunxiao Wang ◽  
Yongnan Liu ◽  
Jing Han ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Acid Biosynthesis ◽  
Ganoderic Acid ◽  
Ceramide Synthases

Dual functions of AreA, a GATA transcription factor, on influencing ganoderic acid biosynthesis in Ganoderma lucidum

2019 ◽  
Vol 21 (11) ◽  
pp. 4166-4179 ◽  
Author(s):  
Jing Zhu ◽  
Zehua Sun ◽  
Dengke Shi ◽  
Shuqi Song ◽  
Lingdan Lian ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Ganoderma Lucidum ◽  
Acid Biosynthesis ◽  
Ganoderic Acid ◽  
Gata Transcription Factor ◽  
Dual Functions
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