Effect of inducible co-overexpression of protein disulfide isomerase and endoplasmic reticulum oxidoreductase on the specific antibody productivity of recombinant Chinese hamster ovary cells

2010 ◽  
Vol 107 (2) ◽  
pp. 337-346 ◽  
Author(s):  
Chaya Mohan ◽  
Gyun Min Lee
Keyword(s):  
Endoplasmic Reticulum ◽  
Specific Antibody ◽  
Chinese Hamster Ovary ◽  
Protein Disulfide Isomerase ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Disulfide Isomerase ◽  
Antibody Productivity ◽  
Protein Disulfide

Reduction of endogenous GRP78 levels improves secretion of a heterologous protein in CHO cells

1988 ◽  
Vol 8 (10) ◽  
pp. 4063-4070
Author(s):  
A J Dorner ◽  
M G Krane ◽  
R J Kaufman
Keyword(s):  
Endoplasmic Reticulum ◽  
Chinese Hamster Ovary ◽  
Heterologous Protein ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Glycosylation Site ◽  
Ovary Cells ◽  
Tissue Plasminogen ◽  
Stable Association

GRP78 is localized in the endoplasmic reticulum and associates with improperly folded or underglycosylated proteins. The role of GRP78 in secretion was studied in Chinese hamster ovary cells expressing a tissue plasminogen activator (tPA) variant which lacks potential N-linked glycosylation site sequences because of mutagenesis. The expression of variant tPA resulted in elevated levels of GRP78 and its stable association with tPA. The introduction of antisense GRP78 genes resulted in a two- to threefold reduction in GRP78 levels compared with those of the original cells. Cells with reduced levels of GRP78 secreted two- to threefold-higher levels of tPA activity. tPA expressed in these cells displayed reduced association with GRP78, and a greater proportion was processed to the mature form and secreted. These results demonstrate that reduction of GRP78 level can improve the secretion of an associated protein.

Effect of the disulfide isomerase PDIa4 on the antibody production of Chinese hamster ovary cells

2020 ◽  
Vol 130 (6) ◽  
pp. 637-643
Author(s):  
Kei Komatsu ◽  
Kento Kumon ◽  
Mayuno Arita ◽  
Masayoshi Onitsuka ◽  
Takeshi Omasa ◽  
...  
Keyword(s):  
Antibody Production ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Disulfide Isomerase

scholarly journals Biological insights into the expression of translation initiation factors from recombinant CHOK1SV cell lines and their relationship to enhanced productivity

2015 ◽  
Vol 472 (3) ◽  
pp. 261-273 ◽  
Author(s):  
Emma J. Mead ◽  
Rosalyn J. Masterton ◽  
Marc Feary ◽  
Olga Obrezanova ◽  
Lin Zhang ◽  
...  
Keyword(s):  
Translation Initiation ◽  
Chinese Hamster Ovary ◽  
Closed Loop ◽  
Chinese Hamster Ovary Cells ◽  
Recombinant Antibody ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Initiation Factors ◽  
Translation Initiation Factors ◽  
Antibody Productivity

We show for translation initiation factors involved in formation of the closed loop mRNA, their expression is associated with recombinant antibody productivity in Chinese hamster ovary cells and maintaining these is important in determining the cells capacity for antibody productivity.

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