scholarly journals Degradation-mediated cellular traction directs stem cell fate in covalently crosslinked three-dimensional hydrogels

2013 ◽  
Vol 12 (5) ◽  
pp. 458-465 ◽  
Author(s):  
Sudhir Khetan ◽  
Murat Guvendiren ◽  
Wesley R. Legant ◽  
Daniel M. Cohen ◽  
Christopher S. Chen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Three Dimensional ◽  
Stem Cell Fate

scholarly journals Biomimetic three-dimensional microenvironment for controlling stem cell fate

2011 ◽  
Vol 1 (5) ◽  
pp. 792-803 ◽  
Author(s):  
Hu Zhang ◽  
Sheng Dai ◽  
Jingxiu Bi ◽  
Kuo-Kang Liu
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Three Dimensional ◽  
Cell Lineage ◽  
Cell Interaction ◽  
Stem Cell Fate ◽  
Cell Microenvironment ◽  
Stem Cell Lineage ◽  
Matrix Cell ◽  
The Individual

Stem cell therapy is an emerging technique which is being translated into treatment of degenerated tissues. However, the success of translation relies on the stem cell lineage commitment in the degenerated regions of interest. This commitment is precisely controlled by the stem cell microenvironment. Engineering a biomimetic three-dimensional microenvironment enables a thorough understanding of the mechanisms of governing stem cell fate. We review the individual microenvironment components, including soluble factors, extracellular matrix, cell–cell interaction and mechanical stimulation. The perspectives in creating the biomimetic microenvironments are discussed with emerging techniques.

scholarly journals Hydrogels Containing Gradients in Vascular Density Reveal Dose-Dependent Role of Angiocrine Cues on Stem Cell Behavior

2021 ◽  
Author(s):  
Mai T. Ngo ◽  
Victoria R. Barnhouse ◽  
Aidan E. Gilchrist ◽  
Christine J. Hunter ◽  
Joy N. Hensold ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Three Dimensional ◽  
Cell Behavior ◽  
Vascular Density ◽  
Microfluidic Mixing ◽  
Hematopoietic Stem ◽  
Stem Cell Fate ◽  
Signaling Axis ◽  
Dose Dependent

AbstractBiomaterials that replicate patterns of microenvironmental signals from the stem cell niche offer the potential to refine platforms to regulate stem cell behavior. While significant emphasis has been placed on understanding the effects of biophysical and biochemical cues on stem cell fate, vascular-derived or angiocrine cues offer an important alternative signaling axis for biomaterial-based stem cell platforms. Elucidating dose-dependent relationships between angiocrine cues and stem cell fate are largely intractable in animal models and two-dimensional cell culture. In this study, we leverage microfluidic mixing devices to generate three-dimensional hydrogels containing lateral gradients in vascular density alongside murine hematopoietic stem cells (HSCs). Regional differences in vascular density can be generated via embossed gradients in cell, matrix, or growth factor density. HSCs co-cultured alongside vascular gradients reveal spatial patterns of HSC phenotype in response to angiocrine signals. Notably, decreased Akt signaling in high vessel density regions led to increased expansion of lineage-positive hematopoietic cells. This approach offers a combinatorial tool to rapidly screen a continuum of microenvironments with varying vascular, biophysical, and biochemical cues to reveal the influence of local angiocrine signals on HSC fate.

Control of three-dimensional substrate stiffness to manipulate mesenchymal stem cell fate toward neuronal or glial lineages

2013 ◽  
Vol 9 (2) ◽  
pp. 5170-5180 ◽  
Author(s):  
Goh Jih Her ◽  
Hsi-Chin Wu ◽  
Ming-Hong Chen ◽  
Ming-Yi Chen ◽  
Shun-Chih Chang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Fate ◽  
Three Dimensional ◽  
Substrate Stiffness ◽  
Stem Cell Fate

Analysis of Chirality Effects on Stem Cell Fate Using Three-dimensional Fibrous Peptide Hydrogels

2018 ◽  
Vol 1 (3) ◽  
pp. 538-543 ◽  
Author(s):  
Hangyu Zheng ◽  
Toru Yoshitomi ◽  
Keitaro Yoshimoto
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Three Dimensional ◽  
Stem Cell Fate ◽  
Peptide Hydrogels
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