scholarly journals Hydrogels as extracellular matrix mimics for 3D cell culture

2009 ◽  
Vol 103 (4) ◽  
pp. 655-663 ◽  
Author(s):  
Mark W. Tibbitt ◽  
Kristi S. Anseth
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
3D Cell Culture

A rhabdomyosarcoma hydrogel model to unveil cell-extracellular matrix interactions

2021 ◽  
Author(s):  
Mattia Saggioro ◽  
Stefania D'Agostino ◽  
Anna Gallo ◽  
Sara Crotti ◽  
Sara D'Aronco ◽  
...  
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
3D Culture ◽  
3D Cell Culture ◽  
Culture Systems ◽  
Matrix Interactions ◽  
In Vitro Systems ◽  
Extracellular Matrix Interactions

Three-dimensional (3D) culture systems are progressively getting attention given their potential in overcoming limitations of the classical 2D in vitro systems. Among different supports for 3D cell culture, hydrogels (HGs)...

scholarly journals Dynamic crosslinked and injectable biohydrogels as extracellular matrix mimics for the delivery of antibiotics and 3D cell culture

RSC Advances ◽  
2020 ◽  
Vol 10 (33) ◽  
pp. 19587-19599 ◽  
Author(s):  
Zhiping Fan ◽  
Ping Cheng ◽  
Min Liu ◽  
Sangeeta Prakash ◽  
Jun Han ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cell Culture ◽  
Extracellular Matrix ◽  
Drug Release ◽  
Release Rate ◽  
3D Cell Culture ◽  
Drug Release Rate ◽  
Sustained Drug Delivery ◽  
Cell Scaffold ◽  
Potential Applications

Polysaccharides-polypeptide derived biohydrogels were formed using hydrazone chemistry as crosslinking strategy, which have controllable drug release rate and many other potential applications, especially in sustained drug delivery and cell scaffold.

Dynamics of Stretch-Induced Stress Fiber Remodeling in 3D Cell Culture

2011 ◽  
Author(s):  
Sheng-Lin Lee ◽  
Ali Nekouzadeh ◽  
Kenneth M. Pryse ◽  
Elliot L. Elson ◽  
Guy M. Genin
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Actin Cytoskeleton ◽  
3D Cell Culture ◽  
Living Cells ◽  
Stress Fiber ◽  
Cell Physiology ◽  
Mechanical Stimuli ◽  
Tissue Development ◽  
Induced Stress

The responses of living cells to mechanical stimuli are believed to underlie diseases such as fibrotic cardiomyopathy [1] and asthma [2]. Emerging evidence suggests that mechanical signals transduced through the actin cytoskeleton and its connections to the extracellular matrix (ECM) have important effects on cell physiology and tissue development [13]. Understanding the responses of cells in realistic mechanical environments to mechanical stimuli is therefore of great importance to understanding development and disease.

Practical Applications of 3D Cell Culture Biotechnologies for Translational Oncology and Personalized Therapy

2020 ◽  
Vol 36 (3) ◽  
pp. 3-15
Author(s):  
D.A. Chudakova ◽  
E.Yu. Skorova ◽  
I.V. Reshetov
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
3D Culture ◽  
3D Cell Culture ◽  
Personalized Therapy ◽  
3D Bioprinting ◽  
Culture Systems ◽  
Culture Models ◽  
2D And 3D

The creation of in vitro three-dimensional cellular model systems (in vitro 3D cultures) is a fast-growing leading-edge segment of the biotechnological industry. We have examined in this work the key 80 articles published after 2008, and focused on applications of in vitro 3D culture in translational oncology. We described a broad range of 3D culture systems, including models with and without extracellular matrix (ECM). 3D culture models based on decellularized ECM were discussed in more detail. The role of ECM in pathogeneis of malignant neoplasms, in particular, in the phenomenon of the tumor resistance to chemotherapy, was evaluated. 2D and 3D culture systems were compared, and natural and synthetic ECM were described, as well as the model creation based on 3D bioprinting. Particular attention was paid to in vitro models of various cancers, including those at the metastatic stage, based on 3D cell cultures, which maximally mimic the in vivo tumor behavior. The prospects of the practical application of 3D cell culture models in preclinical drag screening and in personalized therapy were discussed. We also presented our data on in vitro 2D and 3D culturing of human cells on various substrates. 3D cellular models, 3D bioprinting, biotechnology, extracellular matrix, cancer, translational medicine, personalized medicine, drag development, in vitro, ex vivo, oncology The authors are grateful to Dr. E. Shabalina for providing part of the experimental data and to OKA-Biotech Company for the samples of recombinant Funding-The work was supported by a Grant from the Russian Science Foundation (no. 18-15-00391). doi: 10.21519/0234-2758-2020-36-3-3-15

scholarly journals Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture

10.3791/55094 ◽  
2017 ◽  
Author(s):  
Patrick A. Link ◽  
Robert A. Pouliot ◽  
Nabil S. Mikhaiel ◽  
Bethany M. Young ◽  
Rebecca L. Heise
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
3D Cell Culture
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