Characterization of cyanobacterial β-carotene ketolase and hydroxylase genes inEscherichia coli, and their application for astaxanthin biosynthesis

2009 ◽  
Vol 103 (5) ◽  
pp. 944-955 ◽  
Author(s):  
Mark A. Scaife ◽  
Adam M. Burja ◽  
Phillip C. Wright
Keyword(s):  
Inescherichia Coli ◽  
Β Carotene

Cloning and characterization of a novel β-carotene hydroxylase gene fromLycium barbarumand its expression inEscherichia coli

2014 ◽  
Vol 61 (6) ◽  
pp. 637-645 ◽  
Author(s):  
Jiang Wu ◽  
Jing Ji ◽  
Gang Wang ◽  
Zhaodi Li ◽  
Jinjin Diao ◽  
...  
Keyword(s):  
Inescherichia Coli ◽  
Hydroxylase Gene ◽  
Expression Inescherichia Coli ◽  
Β Carotene

scholarly journals Modulation of Gut Microbiota and Oxidative Status by β-Carotene in Late Pregnant Sows

2020 ◽  
Vol 7 ◽  
Author(s):  
Xupeng Yuan ◽  
Jiahao Yan ◽  
Ruizhi Hu ◽  
Yanli Li ◽  
Ying Wang ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Fecal Microbiota ◽  
Control Group ◽  
Metabolic Activities ◽  
The Impact ◽  
Positive Effect ◽  
Β Carotene

Recent evidences suggest that gut microbiota plays an important role in regulating physiological and metabolic activities of pregnant sows, and β-carotene has a potentially positive effect on reproduction, but the impact of β-carotene on gut microbiota in pregnant sows remains unknown. This study aimed to explore the effect and mechanisms of β-carotene on the reproductive performance of sows from the aspect of gut microbiota. A total of 48 hybrid pregnant sows (Landrace × Yorkshire) with similar parity were randomly allocated into three groups (n = 16) and fed with a basal diet or a diet containing 30 or 90 mg/kg of β-carotene from day 90 of gestation until parturition. Dietary supplementation of 30 or 90 mg/kg β-carotene increased the number of live birth to 11.82 ± 1.54 and 12.29 ± 2.09, respectively, while the control group was 11.00 ± 1.41 (P = 0.201). Moreover, β-carotene increased significantly the serum nitric oxide (NO) level and glutathione peroxidase (GSH-Px) activity (P < 0.05). Characterization of fecal microbiota revealed that 90 mg/kg β-carotene increased the diversity of the gut flora (P < 0.05). In particular, β-carotene decreased the relative abundance of Firmicutes including Lachnospiraceae AC2044 group, Lachnospiraceae NK4B4 group and Ruminococcaceae UCG-008, but enriched Proteobacteria including Bilophila and Sutterella, and Actinobacteria including Corynebacterium and Corynebacterium 1 which are related to NO synthesis. These data demonstrated that dietary supplementation of β-carotene may increase antioxidant enzyme activity and NO, an important vasodilator to promote the neonatal blood circulation, through regulating gut microbiota in sows.

Spectroscopic Characterization of Recombinant Cu,Zn Superoxide Dismutase fromPhotobacterium leiognathiExpressed inEscherichia coli:  Evidence for a Novel Catalytic Copper Binding Site

Biochemistry ◽  
1997 ◽  
Vol 36 (23) ◽  
pp. 7109-7113 ◽  
Author(s):  
Dolly Foti ◽  
Bruno Lo Curto ◽  
Giovanni Cuzzocrea ◽  
M. Elena Stroppolo ◽  
Francesca Polizio ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Binding Site ◽  
Spectroscopic Characterization ◽  
Copper Binding ◽  
Inescherichia Coli

scholarly journals Cloning and characterization of a haloarchaeal heat shock protein 70 functionally expressed inEscherichia coli

2007 ◽  
Vol 275 (1) ◽  
pp. 168-174 ◽  
Author(s):  
Hao Zhang ◽  
Lu Lin ◽  
Chi Zeng ◽  
Ping Shen ◽  
Yu-Ping Huang
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Inescherichia Coli

scholarly journals Cloning, expression, and antigenic characterization of recombinant protein ofMycoplasma gallisepticum expressed inEscherichia coli

2015 ◽  
Vol 94 (4) ◽  
pp. 621-627 ◽  
Author(s):  
T.S. Rocha ◽  
C. Tramuta ◽  
S. Catania ◽  
A. Matucci ◽  
M.G. Giuffrida ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Inescherichia Coli ◽  
Antigenic Characterization

Characterization of anAspergillus oryzaeCysteinyl Dipeptidase Expressed inEscherichia coli

2011 ◽  
Vol 75 (1) ◽  
pp. 159-161 ◽  
Author(s):  
Ryota HATTORI ◽  
Mayumi MATSUSHITA-MORITA ◽  
Junichiro MARUI ◽  
Sawaki TADA ◽  
Satoshi SUZUKI ◽  
...  
Keyword(s):  
Inescherichia Coli

Further characterization of the two catalases inEscherichia coli

1985 ◽  
Vol 12 (6) ◽  
pp. 315-319 ◽  
Author(s):  
Efrat Meir ◽  
Ezra Yagil
Keyword(s):  
Inescherichia Coli

Purification and Characterization of Recombinant Osteocalcin Fusion Protein Expressed inEscherichia coli

1996 ◽  
Vol 8 (2) ◽  
pp. 137-144 ◽  
Author(s):  
Sanna-Maria Käkönen ◽  
Jukka Hellman ◽  
Kim Pettersson ◽  
Timo Lövgren ◽  
Matti Karp
Keyword(s):  
Fusion Protein ◽  
Inescherichia Coli ◽  
Purification And Characterization
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