scholarly journals Cloning, expression, and antigenic characterization of recombinant protein ofMycoplasma gallisepticum expressed inEscherichia coli

2015 ◽  
Vol 94 (4) ◽  
pp. 621-627 ◽  
Author(s):  
T.S. Rocha ◽  
C. Tramuta ◽  
S. Catania ◽  
A. Matucci ◽  
M.G. Giuffrida ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Inescherichia Coli ◽  
Antigenic Characterization

scholarly journals Antigenic Characterization of ORF2 and ORF3 Proteins of Hepatitis E Virus (HEV)

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1385
Author(s):  
Giulia Pezzoni ◽  
Lidia Stercoli ◽  
Eleonora Pegoiani ◽  
Emiliana Brocchi
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Recombinant Antigen ◽  
Open Reading Frame ◽  
Reading Frame ◽  
E Coli ◽  
Antigenic Characterization ◽  
Antigenic Properties ◽  
Open Reading Frame 2

To evaluate the antigenic properties of Hepatitis E Virus (HEV) Open Reading Frame 2 and 3 (ORF2 and ORF3) codified proteins, we expressed different portions of ORF2 and the entire ORF3 in E. coli, a truncated ORF2, was also expressed in baculovirus. A panel of 37 monoclonal antibodies (MAbs) was raised against ORF2 (1–660 amino acids) and MAbs were mapped and characterized using the ORF2 expressed portions. Selected HEV positive and negative swine sera were used to evaluate ORF2 and ORF3 antigens’ immunogenicity. The MAbs were clustered in six groups identifying six antigenic regions along the ORF2. Only MAbs binding to the sixth ORF2 antigenic region (394–608 aa) were found to compete with HEV positive sera and efficiently catch the recombinant antigen expressed in baculovirus. The ORF2 portion from 394–608 aa demonstrated to include most immunogenic epitopes with 85% of HEV positive swine sera reacting against the region from 461–544 aa. Only 5% of the selected HEV sera reacted against the ORF3 antigen.

scholarly journals Antigenic characterization of novel H1 influenza A viruses in swine

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Rodrigo Tapia ◽  
Montserrat Torremorell ◽  
Marie Culhane ◽  
Rafael A. Medina ◽  
Víctor Neira
Keyword(s):  
Influenza A ◽  
Influenza A Viruses ◽  
Antigenic Characterization

Spectroscopic Characterization of Recombinant Cu,Zn Superoxide Dismutase fromPhotobacterium leiognathiExpressed inEscherichia coli:  Evidence for a Novel Catalytic Copper Binding Site

Biochemistry ◽  
1997 ◽  
Vol 36 (23) ◽  
pp. 7109-7113 ◽  
Author(s):  
Dolly Foti ◽  
Bruno Lo Curto ◽  
Giovanni Cuzzocrea ◽  
M. Elena Stroppolo ◽  
Francesca Polizio ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Binding Site ◽  
Spectroscopic Characterization ◽  
Copper Binding ◽  
Inescherichia Coli

scholarly journals Cloning and characterization of a haloarchaeal heat shock protein 70 functionally expressed inEscherichia coli

2007 ◽  
Vol 275 (1) ◽  
pp. 168-174 ◽  
Author(s):  
Hao Zhang ◽  
Lu Lin ◽  
Chi Zeng ◽  
Ping Shen ◽  
Yu-Ping Huang
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Inescherichia Coli

Antigenic Characterization of an Arkansas Isolate of Infectious Bursal Disease Virus

1996 ◽  
Vol 40 (3) ◽  
pp. 588 ◽  
Author(s):  
G. R. Bayyari ◽  
J. D. Story ◽  
J. N. Beasley ◽  
J. K. Skeeles
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Antigenic Characterization ◽  
Bursal Disease

Characterization of anAspergillus oryzaeCysteinyl Dipeptidase Expressed inEscherichia coli

2011 ◽  
Vol 75 (1) ◽  
pp. 159-161 ◽  
Author(s):  
Ryota HATTORI ◽  
Mayumi MATSUSHITA-MORITA ◽  
Junichiro MARUI ◽  
Sawaki TADA ◽  
Satoshi SUZUKI ◽  
...  
Keyword(s):  
Inescherichia Coli
Sign in / Sign up

Export Citation Format

Share Document