Host strain influences on supercoiled plasmid DNA production inEscherichia coli: Implications for efficient design of large-scale processes

2008 ◽  
Vol 101 (3) ◽  
pp. 529-544 ◽  
Author(s):  
Sin Yee Yau ◽  
Eli Keshavarz-Moore ◽  
John Ward
Keyword(s):  
Plasmid Dna ◽  
Large Scale ◽  
Host Strain ◽  
Inescherichia Coli ◽  
Efficient Design ◽  
Supercoiled Plasmid ◽  
Dna Production

scholarly journals An improved method for large-scale preparation of negatively and positively supercoiled plasmid DNA

BioTechniques ◽  
2009 ◽  
Vol 47 (1) ◽  
pp. 633-635 ◽  
Author(s):  
Marita C. Barth ◽  
Debra A. Dederich ◽  
Peter C. Dedon
Keyword(s):  
Plasmid Dna ◽  
Large Scale ◽  
Improved Method ◽  
Supercoiled Plasmid ◽  
Large Scale Preparation ◽  
Scale Preparation

Large-scale plasmid DNA processing: evidence that cell harvesting and storage methods affect yield of supercoiled plasmid DNA

2008 ◽  
Vol 51 (1) ◽  
pp. 43 ◽  
Author(s):  
Simyee Kong ◽  
Cassandra F. Rock ◽  
Andrew Booth ◽  
Nicholas Willoughby ◽  
Ronan D. O'Kennedy ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Large Scale ◽  
Supercoiled Plasmid ◽  
Cell Harvesting ◽  
Storage Methods ◽  
And Storage ◽  
Dna Processing

A widespread pathway for substitution of adenine by diaminopurine in phage genomes

Science ◽  
2021 ◽  
Vol 372 (6541) ◽  
pp. 512-516
Author(s):  
Yan Zhou ◽  
Xuexia Xu ◽  
Yifeng Wei ◽  
Yu Cheng ◽  
Yu Guo ◽  
...  
Keyword(s):  
Large Scale ◽  
Restriction Enzymes ◽  
Diverse Range ◽  
Host Restriction ◽  
Form And Function ◽  
Multienzyme System ◽  
Dna Modifications ◽  
Dna Production ◽  
And Function

DNA modifications vary in form and function but generally do not alter Watson-Crick base pairing. Diaminopurine (Z) is an exception because it completely replaces adenine and forms three hydrogen bonds with thymine in cyanophage S-2L genomic DNA. However, the biosynthesis, prevalence, and importance of Z genomes remain unexplored. Here, we report a multienzyme system that supports Z-genome synthesis. We identified dozens of globally widespread phages harboring such enzymes, and we further verified the Z genome in one of these phages, Acinetobacter phage SH-Ab 15497, by using liquid chromatography with ultraviolet and mass spectrometry. The Z genome endows phages with evolutionary advantages for evading the attack of host restriction enzymes, and the characterization of its biosynthetic pathway enables Z-DNA production on a large scale for a diverse range of applications.

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