scholarly journals An improved method for large-scale preparation of negatively and positively supercoiled plasmid DNA

BioTechniques ◽  
2009 ◽  
Vol 47 (1) ◽  
pp. 633-635 ◽  
Author(s):  
Marita C. Barth ◽  
Debra A. Dederich ◽  
Peter C. Dedon
Keyword(s):  
Plasmid Dna ◽  
Large Scale ◽  
Improved Method ◽  
Supercoiled Plasmid ◽  
Large Scale Preparation ◽  
Scale Preparation

A continuous thermal lysis procedure for the large-scale preparation of plasmid DNA

2005 ◽  
Vol 118 (3) ◽  
pp. 257-264 ◽  
Author(s):  
Kaichun Zhu ◽  
Huali Jin ◽  
Yijie Ma ◽  
Zhihui Ren ◽  
Chong Xiao ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Large Scale ◽  
Large Scale Preparation ◽  
Scale Preparation

Repeated Use of Qiagen Columns in Large-Scale Preparation of Plasmid DNA

DNA Vaccines ◽  
2003 ◽  
pp. 35-36
Author(s):  
Derek Gregory ◽  
Ricardo E. Tascon ◽  
Douglas B. Lowrie
Keyword(s):  
Plasmid Dna ◽  
Large Scale ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Repeated Use

An Improved Method of Synthesis of per-6-deoxy-6-iodo-β-cyclodextrin

2019 ◽  
Vol 41 (2) ◽  
pp. 356-356
Author(s):  
Suomin Feng Suomin Feng ◽  
Jiaxing Feng Jiaxing Feng ◽  
Guangcan Xu Guangcan Xu ◽  
Jiang Liu Jiang Liu ◽  
and Yongmin Zhang and Yongmin Zhang
Keyword(s):  
Chemical Synthesis ◽  
Supramolecular Chemistry ◽  
Experimental Verification ◽  
Large Scale ◽  
Synthesis Reaction ◽  
Cyclodextrin Derivatives ◽  
Improved Method ◽  
Operation Process ◽  
Large Scale Preparation ◽  
Scale Preparation

A number of per-6-substituted-6-deoxy-β-cyclodextrin derivatives have been synthesized for many years, of them, per-6-deoxy-6-iodo-β-cyclodextrin is of great importance in supramolecular chemistry. By reviewing all published papers related to synthesis of per-6-deoxy-6-iodo-β-cyclodextrin and combining with our experimental verification, this work has reported an improved method of synthesis of per-6-deoxy-6-iodo-β-cyclodextrin. The approach mainly uses adding a certain amount of methanol instead of removing DMF before adjusting pH. We think this project is simple, efficient, high repeatable and suitable for large-scale preparation, superior even to all the previously reported methods. The synthesis of bromo, chloro substituted -per-6-β-CD may also refer to this improved method. At the same time, the detailed introduction of operation process and precautions will serve as a reference and guidance to the beginner who will engage in the study on the chemical synthesis reaction of sugar and cyclodextrin.

Large‐Scale Preparation of Plasmid DNA

1998 ◽  
Vol 41 (1) ◽  
Author(s):  
J.S. Heilig ◽  
Karen L. Elbing ◽  
Roger Brent
Keyword(s):  
Plasmid Dna ◽  
Large Scale ◽  
Large Scale Preparation ◽  
Scale Preparation

Purification of the Antihemophilic Factor by Gel Filtration on Agarose

1969 ◽  
Vol 22 (03) ◽  
pp. 577-583 ◽  
Author(s):  
M.M.P Paulssen ◽  
A.C.M.G.B Wouterlood ◽  
H.L.M.A Scheffers
Keyword(s):  
Factor Viii ◽  
Plasma Proteins ◽  
Large Scale ◽  
Gel Filtration ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Antihemophilic Factor

SummaryFactor VIII can be isolated from plasma proteins, including fibrinogen by chromatography on agarose. The best results were obtained with Sepharose 6B. Large scale preparation is also possible when cryoprecipitate is separated by chromatography. In most fractions containing factor VIII a turbidity is observed which may be due to the presence of chylomicrons.The purified factor VIII was active in vivo as well as in vitro.

The occurrence of long chain polyprenols in leaves of plants of Rosaceae family and their isolation by time-extended liquid chromatography

1992 ◽  
Vol 70 (6) ◽  
pp. 448-454 ◽  
Author(s):  
Ewa Świeżewska ◽  
T. Chojnacki ◽  
W. J. Jankowski ◽  
K. Singh ◽  
J. Olsson
Keyword(s):  
Liquid Chromatography ◽  
Fatty Acid ◽  
Large Scale ◽  
Fatty Acid Esters ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Wet Weight ◽  
Rosaceae Family ◽  
Acid Esters ◽  
Long Chain

The long chain polyprenols composed of 30 and more isoprene units from leaves of plants belonging to the genera Potentilla and Rosa have been described. They occur in the form of fatty acid esters. The composition of polyprenol mixture was species dependent and its content reached ca. 0.5% wet weight. Large scale preparation of individual polyprenols from a natural polyprenol mixture was performed using time-extended liquid chromatography on the hydrophobic gel Lipidex-5000.Key words: long chain polyprenols, Rosaceae.

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