High concentrations of clavulanic acid but not of its degradation products decrease glycerol consumption and oxygen uptake rates in cultures ofStreptomyces clavuligerus

2008 ◽  
Vol 100 (3) ◽  
pp. 439-447 ◽  
Author(s):  
Simone Brethauer ◽  
Martin Held ◽  
Sven Panke
Keyword(s):  
Oxygen Uptake ◽  
Clavulanic Acid ◽  
Degradation Products ◽  
Uptake Rates ◽  
High Concentrations ◽  
Glycerol Consumption

THE METABOLISM OF NORMAL BRAIN AND HUMAN GLIOMAS IN RELATION TO CELL TYPE AND DENSITY

1955 ◽  
Vol 33 (3) ◽  
pp. 395-403 ◽  
Author(s):  
Irving H. Heller ◽  
K. A. C. Elliott
Keyword(s):  
Cerebral Cortex ◽  
White Matter ◽  
Brain Tumors ◽  
Corpus Callosum ◽  
Oxygen Uptake ◽  
Glial Cells ◽  
Cerebellar Cortex ◽  
Unit Weight ◽  
Uptake Rates ◽  
The Brain

Per unit weight, cerebral and cerebellar cortex respire much more actively than corpus callosum. The rate per cell nucleus is highest in cerebral cortex, lower in corpus callosum, and still lower in cerebellar cortex. The oxygen uptake rates of the brain tumors studied, with the exception of an oligodendroglioma, were about the same as that of white matter on the weight basis but lower than that of cerebral cortex or white matter on the cell basis. In agreement with previous work, an oligodendroglioma respired much more actively than the other tumors. The rates of glycolysis of the brain tumors per unit weight were low but, relative to their respiration rate, glycolysis was higher than in normal gray or white matter. Consideration of the figures obtained leads to the following tentative conclusions: Glial cells of corpus callosum respire more actively than the neurons of the cerebellar cortex. Neurons of the cerebral cortex respire on the average much more actively than neurons of the cerebellar cortex or glial cells. Considerably more than 70% of the oxygen uptake by cerebral cortex is due to neurons. The oxygen uptake rates of normal oligodendroglia and astrocytes are probably about the same as the rates found per nucleus in an oligodendroglioma and in astrocytomas; oligodendroglia respire much more actively than astrocytes.

scholarly journals Cultivation at high osmotic pressure confers ubiquinone 8–independent protection of respiration on Escherichia coli

2019 ◽  
Vol 295 (4) ◽  
pp. 981-993 ◽  
Author(s):  
Laura Tempelhagen ◽  
Anita Ayer ◽  
Doreen E. Culham ◽  
Roland Stocker ◽  
Janet M. Wood
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Escherichia Coli ◽  
Electron Transfer ◽  
Oxygen Uptake ◽  
Osmotic Pressure ◽  
Respiratory Chain ◽  
Membrane Lipid ◽  
E Coli ◽  
Uptake Rates

Ubiquinone 8 (coenzyme Q8 or Q8) mediates electron transfer within the aerobic respiratory chain, mitigates oxidative stress, and contributes to gene expression in Escherichia coli. In addition, Q8 was proposed to confer bacterial osmotolerance by accumulating during growth at high osmotic pressure and altering membrane stability. The osmolyte trehalose and membrane lipid cardiolipin accumulate in E. coli cells cultivated at high osmotic pressure. Here, Q8 deficiency impaired E. coli growth at low osmotic pressure and rendered growth osmotically sensitive. The Q8 deficiency impeded cellular O2 uptake and also inhibited the activities of two proton symporters, the osmosensing transporter ProP and the lactose transporter LacY. Q8 supplementation decreased membrane fluidity in liposomes, but did not affect ProP activity in proteoliposomes, which is respiration-independent. Liposomes and proteoliposomes prepared with E. coli lipids were used for these experiments. Similar oxygen uptake rates were observed for bacteria cultivated at low and high osmotic pressures. In contrast, respiration was dramatically inhibited when bacteria grown at the same low osmotic pressure were shifted to high osmotic pressure. Thus, respiration was restored during prolonged growth of E. coli at high osmotic pressure. Of note, bacteria cultivated at low and high osmotic pressures had similar Q8 concentrations. The protection of respiration was neither diminished by cardiolipin deficiency nor conferred by trehalose overproduction during growth at low osmotic pressure, but rather might be achieved by Q8-independent respiratory chain remodeling. We conclude that osmotolerance is conferred through Q8-independent protection of respiration, not by altering physical properties of the membrane.

Engineering aspects of a mixed methanotrophic culture in a membrane-aerated biofilm reactor

2004 ◽  
Vol 49 (11-12) ◽  
pp. 255-262 ◽  
Author(s):  
E. Casey ◽  
S. Rishell ◽  
B. Glennon ◽  
G. Hamer
Keyword(s):  
Oxygen Uptake ◽  
Biofilm Reactor ◽  
Specific Rate ◽  
Suspended Cell ◽  
Biofilm Thickness ◽  
Biofilm Reactors ◽  
Uptake Rates ◽  
Methane Uptake ◽  
Cell Processes ◽  
Oxidation Efficiency

Methanotrophic biodegradation using the membrane-aerated biofilm reactor (MABR) is a technology offering several advantages over both conventional biofilm reactors and suspended-cell processes. In this study the oxidation efficiency of a methanotrophic biofilm in a 1.5 litre MABR was investigated. Measurements of oxygen and methane uptake rates together with biofilm thickness were taken for developing biofilms. It was found that the specific rate of metabolic activity of the biofilm was unusually high as determined by the methane and oxygen uptake rates. Microbial activity stratification was evident and the location of stratified layers of oxygen consuming components of the consortium could be manipulated via the intra-membrane oxygen pressure.

Fixed Biomass Activity in Aerobic Biological Reactors Used for Wastewater Treatment

1988 ◽  
Vol 20 (11-12) ◽  
pp. 489-491
Author(s):  
J. C. Block ◽  
N. Nouvion ◽  
J. M. Cahen ◽  
Y. Lesty ◽  
G. M. Faup
Keyword(s):  
Wastewater Treatment ◽  
Oxygen Uptake ◽  
Fixed Bed ◽  
Fixed Bed Reactor ◽  
Biomass Growth ◽  
Head Losses ◽  
Uptake Rates ◽  
Biomass Activity ◽  
Potential Activity ◽  
Experimental Runs

Experimental runs were carried out on an upflow fixed-bed reactor. The process cycle of 24 hours was determined by following head losses used as an indicator of the clogging of the filter and of the biomass growth. Thanks to a protocol of dispersion of the biomass present into the filter, two fractions defined as fixed and shared biomass were obtained. The specific dehydrogenase activities or the specific oxygen uptake rates of the dispersed biomass were always greater than those of the fixed biomass. The aggregation of the biomass into the reactor prevents its potential activity being used to the full.

scholarly journals Estimating the impact of inhibitory substances on activated sludge denitrification process

2019 ◽  
Vol 14 (4) ◽  
pp. 863-871 ◽  
Author(s):  
Kati Klein ◽  
Taavo Tenno
Keyword(s):  
Standard Deviation ◽  
Oxygen Uptake ◽  
Activated Sludge ◽  
Process Efficiency ◽  
Sludge Treatment ◽  
Conventional Activated Sludge ◽  
Uptake Rates ◽  
Relative Standard ◽  
The Impact ◽  
Denitrification Process

Abstract Industrial wastewater frequently contains substances which inhibit activated sludge treatment processes. Inhibitory characteristics of different substances are usually evaluated based on testing the impact of respective substance on activated sludge nitrification or oxygen uptake rates. However, denitrification is always before aerobic processes in conventional activated sludge treatment plants and thereby more exposed to inhibitory compounds. There is no easily applicable and validated method available for determination of denitrification process efficiency and inhibition. In this study, a method for evaluation of inhibition on the activated sludge denitrification process was developed and validated using 3,5-dichlorophenol (3,5-DCP) as a model inhibitory compound and additionally controlled with real wastewater produced in the shale oil industry. Average IC50 value (5.5 ± 2.2 mg L−1) for 3,5-DCP showed that denitrifiers were less sensitive than nitrifiers (IC50 = 2.9 ± 0.7 mg L−1) and more sensitive than aerobic heterotrophs (IC50 = 7.2 ± 2.4 mg L−1). Methodological aspects like accumulation of nitrite nitrogen, acclimatization of biomass and technical issues were discussed. Achieved validation characteristics were similar with ISO Standards estimating activated sludge nitrification and oxygen uptake rates, which proves the reliability of the method: standard deviation, 95.4% confidence level, relative standard deviation were calculated to be 2.2 mg L−1, 1.2 … 9.8 mg L−1 and 39.2%, respectively.

The Behaviour, Survival and Respiration of the Cockles Cerastoderma Edule and C. Glaucum in Air

1972 ◽  
Vol 52 (3) ◽  
pp. 661-680 ◽  
Author(s):  
C. R. Boyden
Keyword(s):  
Oxygen Uptake ◽  
Behaviour Pattern ◽  
Air Breathing ◽  
Cerastoderma Edule ◽  
Anoxic Conditions ◽  
Metabolic Scope ◽  
Uptake Rates

Aspects of the physiology of the two cockles Cerastoderma edule (L.) and C. glaucum (Poiret) in air have been investigated. Both cockles exhibit bradycardia during exposure and are similarly tolerant of anoxic conditions, but C. edule survives longer in air than C. glaucum. This is accounted for by the fact that C. edule displays a behaviour pattern of valve movements upon emersion which allows air-breathing. Oxygen uptake rates of this cockle measured in air lie close to the lowest rates recorded in water, and are considered to approximate to a basal or quiescent level. C. glaucum does not air-breathe and is restricted to lowest shore levels. Values for ‘maximal’ levels of oxygen uptake in water have also been obtained together with estimates of metabolic scope for the cockle.

scholarly journals Oxygen uptake rates in cultured rat hepatocytes

1992 ◽  
Vol 40 (10) ◽  
pp. 1286-1291 ◽  
Author(s):  
Avi Rotem ◽  
Menmet Toner ◽  
Ronald G. Tompkins ◽  
Martin L. Yarmush
Keyword(s):  
Oxygen Uptake ◽  
Rat Hepatocytes ◽  
Uptake Rates ◽  
Cultured Rat Hepatocytes

Oxygen Transfer Efficiency Measurements Using Off-Gas Techniques

1989 ◽  
Vol 21 (10-11) ◽  
pp. 1295-1300 ◽  
Author(s):  
W. C. Boyle ◽  
B. G. Hellstrom ◽  
L. Ewing
Keyword(s):  
Oxygen Uptake ◽  
Uptake Rate ◽  
Oxygen Transfer ◽  
Oxygen Uptake Rate ◽  
Transfer Efficiency ◽  
Theoretical Development ◽  
Process Conditions ◽  
Uptake Rates ◽  
Oxygen Transfer Efficiency ◽  
On Line

The off-gas technique for measuring oxygen transfer efficiency in aeration tanks under process conditions was proposed as an accurate technique for calibrating or verifying on-line methods used to estimate oxygen uptake rates. The theoretical development of the off-gas technique was presented. Application of this technique in verifying or calibrating existing on-line methods for estimating oxygen uptake rate was presented. Direct application as an on-line method for estimating oxygen uptake rate was also discussed.

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