scholarly journals Oxygen uptake rates in cultured rat hepatocytes

1992 ◽  
Vol 40 (10) ◽  
pp. 1286-1291 ◽  
Author(s):  
Avi Rotem ◽  
Menmet Toner ◽  
Ronald G. Tompkins ◽  
Martin L. Yarmush
Keyword(s):  
Oxygen Uptake ◽  
Rat Hepatocytes ◽  
Uptake Rates ◽  
Cultured Rat Hepatocytes

Identification and characterization of a basolateral dicarboxylate/cholate antiport system in rat hepatocytes

1995 ◽  
Vol 268 (5) ◽  
pp. G797-G805 ◽  
Author(s):  
U. A. Boelsterli ◽  
B. Zimmerli ◽  
P. J. Meier
Keyword(s):  
Plasma Membrane ◽  
Driving Forces ◽  
Organic Anion ◽  
Rat Hepatocytes ◽  
Disulfonic Acid ◽  
Uptake Rates ◽  
Basolateral Plasma Membrane ◽  
Cultured Rat Hepatocytes ◽  
Extracellular Sodium ◽  
Stimulation Of

The mechanisms and driving forces for the uptake of the unconjugated bile acid cholate were investigated both in cultured rat hepatocytes and in rat liver basolateral (sinusoidal) plasma membrane (BLPM) vesicles. Determination of initial uptake rates of [3H]cholate (0.1 microM) into cultured hepatocytes confirmed that the majority (75%) of the transmembrane transport was mediated by Na(+)-independent mechanisms. This portion of cholate uptake consisted of a pH-sensitive moiety representing nonionic diffusion, which may become quantitatively important at low pH and high cholate concentrations, as well as of a saturable (Michaelis constant 7.4 microM), 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS)-sensitive transport moiety, suggesting the involvement of a carrier. This latter transport system was functionally characterized by 1) inhibition of cellular cholate uptake in the absence of extracellular sodium by the dicarboxylic acid alpha-ketoglutarate (alpha-KG; 1 mM) and by the organic anion p-aminohippurate (PAH; 1 mM); 2) stimulation of cellular cholate uptake by alpha-KG (10 microM) or PAH (1 mM) in the presence of an inwardly directed sodium gradient; 3) lack of sensitivity toward lithium in BLPM vesicles; 4) trans-stimulation of vesicular cholate uptake by alpha-KG or PAH, but not by benzoate; and 5) cis-inhibition of alpha-KG/alpha-KG self-exchange by extravesicular cholate (400 microM), PAH (5 mM), probenecid, or DIDS. Collectively, these data indicate the presence of a Na(+)-dicarboxylate cotransport-coupled organic anion exchanger in the hepatocyte basolateral plasma membrane that may be involved in cholate uptake in the liver.(ABSTRACT TRUNCATED AT 250 WORDS)

Nutrition and somatomedin. XXVI. Molecular regulation of IGF-I by insulin in cultured rat hepatocytes

Diabetes ◽  
1991 ◽  
Vol 40 (11) ◽  
pp. 1525-1530 ◽  
Author(s):  
L. S. Phillips ◽  
S. Goldstein ◽  
C. I. Pao
Keyword(s):  
Rat Hepatocytes ◽  
Molecular Regulation ◽  
Igf I ◽  
Cultured Rat Hepatocytes

scholarly journals Bile acid secretion by cultured rat hepatocytes. Regulation by cholesterol availability.

1983 ◽  
Vol 258 (6) ◽  
pp. 3661-3667 ◽  
Author(s):  
R A Davis ◽  
P M Hyde ◽  
J C Kuan ◽  
M Malone-McNeal ◽  
J Archambault-Schexnayder
Keyword(s):  
Bile Acid ◽  
Acid Secretion ◽  
Rat Hepatocytes ◽  
Cultured Rat Hepatocytes ◽  
Bile Acid Secretion
Sign in / Sign up

Export Citation Format

Share Document