Production and separation of manganese peroxidase from heme amended yeast cultures

2007 ◽  
Vol 99 (3) ◽  
pp. 540-549 ◽  
Author(s):  
Fei Jiang ◽  
Puapong Kongsaeree ◽  
Rose Charron ◽  
Curtis Lajoie ◽  
Haowen Xu ◽  
...  
Keyword(s):  
Manganese Peroxidase ◽  
Yeast Cultures

Biodegradation and decolorization of melanoidin solutions by manganese peroxidase yeasts

2016 ◽  
Vol 73 (10) ◽  
pp. 2436-2445 ◽  
Author(s):  
Samir Mahgoub ◽  
Costas Tsioptsias ◽  
Petros Samaras
Keyword(s):  
Organic Matter ◽  
Manganese Peroxidase ◽  
28S Rrna ◽  
Mixed Liquor ◽  
Removal Capacity ◽  
Yeast Cultures ◽  
Yeast Strains ◽  
Solid Agar ◽  
Yeast Extract Medium ◽  
Aseptic Conditions

The ability of selected manganese peroxidase (MnP) yeast strains, isolated from the mixed liquor of an activated sludge bioreactor treating melanoidins wastewater, was investigated in this work, aiming to examine the degradation potential of melanoidins, in the presence or absence of nutrients. Ten yeast strains were initially isolated from the mixed liquor; four yeast strains (Y1, Y2, Y3 and Y4) were selected for further studies, based on their tolerance towards synthetic melanoidins (SMs) degradation and MnP activity onto solid agar medium. The Y1 strain exhibited almost 98% homology to Candida glabrata yeast, based on 28S rRNA identification studies. During experiments carried out using SM at 30 °C, the four isolated yeast cultures showed a noticeable organic matter reduction and decolorization capacity reaching up to 70% within 2–5 days. However, the corresponding yeast cultures grown in glucose peptone yeast extract medium using real melanoidin wastewater at 30°C showed lower organic matter and color removal capacity, reaching about 60% within 2–5 days. Nevertheless, it was found that the removal of real and synthetic melanoidins could be carried out by these strains under non-aseptic conditions, without requiring further addition of nutrients.

scholarly journals Manganese Peroxidase from the Lignin-degrading Basidiomycete Phanerochaete chrysosporium

1989 ◽  
Vol 264 (6) ◽  
pp. 3335-3340 ◽  
Author(s):  
H Wariishi ◽  
H B Dunford ◽  
I D MacDonald ◽  
M H Gold
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Manganese Peroxidase

A CONVENIENT METHOD OF OBTAINING ASCOSPORES FROM BAKERS' YEAST

1949 ◽  
Vol 27c (4) ◽  
pp. 179-189 ◽  
Author(s):  
A. M. Adams
Keyword(s):  
Convenient Method ◽  
Sodium Acetate ◽  
Solid Medium ◽  
Genetic Research ◽  
Yeast Cells ◽  
Sporulation Medium ◽  
Direct Transfer ◽  
Yeast Cultures ◽  
High Yields ◽  
Solid Nutrient Medium

The superiority of methods involving the use of sporulation media containing acetate, first introduced by Stantial and Elder, over several commonly employed methods is established. A new method for obtaining ascospores from bakers' yeast cultures is recommended involving the direct transfer of vegetative cells from a solid nutrient medium to a solid medium containing acetate. High yields of ascospores are consistently produced after seven days' incubation. This method should lend itself particularly to use in the preparation of ascospores for instructional work, and for genetic research in yeast, and may also find application in yeast taxonomy. The technique recommended is as follows: vegetative yeast cells are multiplied on tomato juice agar or on dextrose nutrient agar, and are then transferred to a solid sporulation medium containing 0.04% dextrose, 0.14% anhydrous sodium acetate, and 2% agar.

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