Stable production of hyaluronic acid inStreptococcus zooepidemicus chemostats operated at high dilution rate

2005 ◽  
Vol 90 (6) ◽  
pp. 685-693 ◽  
Author(s):  
Lars M. Blank ◽  
Richard L. McLaughlin ◽  
Lars K. Nielsen
Keyword(s):  
Hyaluronic Acid ◽  
Dilution Rate ◽  
High Dilution ◽  
Stable Production ◽  
High Dilution Rate

Evolution of Fusarium graminearum A3/5 grown in a series of glucose-limited chemostat cultures at a high dilution rate

1995 ◽  
Vol 99 (2) ◽  
pp. 173-178 ◽  
Author(s):  
Marilyn G. Wiebe ◽  
Geoffrey D. Robson ◽  
Stephen G. Oliver ◽  
Anthony P.J. Trinci
Keyword(s):  
Dilution Rate ◽  
Fusarium Graminearum ◽  
High Dilution ◽  
Chemostat Cultures ◽  
High Dilution Rate

scholarly journals Effects of the antibiotic monensin and an inhibitor of methanogenesis on in vitro continuous rumen fermentations

1981 ◽  
Vol 45 (3) ◽  
pp. 567-578 ◽  
Author(s):  
G. Stanier ◽  
A. Davies
Keyword(s):  
Fatty Acids ◽  
Dilution Rate ◽  
Rate Control ◽  
Volatile Fatty Acids ◽  
High Dilution ◽  
In Vitro System ◽  
Practical Implications ◽  
High Dilution Rate

1. The effects of a methane inhibitor, ICI 111075, and a propionate enhancer, monensin, were studied using in vitro continuous fermenters.2. Both compounds increased the yield of substrate energy, carbon and hydrogen in volatile fatty acids (VFA). This was mainly due to an increase in the molar proportion of propionic acid.3. Improved yields of VFA were accompanied by reductions in methane production and microbial yield.4. Since published information showed that monensin reduced rumen dilution rate in vivo an analogous in vitro system was proposed in which a high dilution rate control fermenter was compared with a monensin treated fermenter set to run at a low dilution rate.5.Results showed that the general intrinsic microbial activity of the chemical manipulators was not affected by changes in dilution rate. Changing dilution rate in addition to chemical treatment however resulted in substantial modifications in the net effect on the fermentation.6. The practical implications of reducing rumen dilution rate as a side effect of chemically manipulating the rumen fermentation could involve changes in food intake, increased importance of secondary fermentations and a reduced effect of nutrients not degraded in the rumen.

scholarly journals Chemostat Production of Plantaricin C ByLactobacillus plantarum LL441

1998 ◽  
Vol 64 (9) ◽  
pp. 3512-3514 ◽  
Author(s):  
J. M. Bruno Bárcena ◽  
F. Siñeriz ◽  
D. González de Llano ◽  
Ana Rodríguez ◽  
Juan E. Suárez
Keyword(s):  
Carbon Source ◽  
Dilution Rate ◽  
Lactobacillus Plantarum ◽  
Carbon Sources ◽  
High Dilution

ABSTRACT Plantaricin C, a bacteriocin synthesized by Lactobacillus plantarum LL441, was optimally produced in chemostats kept at pH 5.0, 30°C, 150 rpm, and a dilution rate of 0.05 h−1 when glucose was used as carbon source and a dilution rate of 0.10 to 0.12 h−1 when sucrose or fructose was used instead. Production was abolished at high dilution rates, i.e., when the cells grew rapidly in all carbon sources.

scholarly journals Effect of Dilution Rate on Metabolic Pathway Shift between Aceticlastic and Nonaceticlastic Methanogenesis in Chemostat Cultivation

2004 ◽  
Vol 70 (7) ◽  
pp. 4048-4052 ◽  
Author(s):  
Toru Shigematsu ◽  
Yueqin Tang ◽  
Tsutomu Kobayashi ◽  
Hiromi Kawaguchi ◽  
Shigeru Morimura ◽  
...  
Keyword(s):  
Dilution Rate ◽  
Phylogenetic Analyses ◽  
Gas Chromatography Mass Spectrometry ◽  
High Dilution ◽  
Chemostat Cultivation ◽  
Reductase Gene ◽  
Syntrophic Oxidation ◽  
Hydrogenotrophic Methanogens ◽  
Methyl Coenzyme M Reductase ◽  
Limited Coverage

ABSTRACT Acetate conversion pathways of methanogenic consortia in acetate-fed chemostats at dilution rates of 0.025 and 0.6 day−1 were investigated by using 13C-labeled acetates, followed by gas chromatography-mass spectrometry (GC-MS) analysis of the CH4 and CO2 produced. Nonaceticlastic syntrophic oxidation by acetate-oxidizing syntrophs and hydrogenotrophic methanogens was suggested to occupy a primary pathway (approximately 62 to 90%) in total methanogenesis at the low dilution rate. In contrast, aceticlastic cleavage of acetate by aceticlastic methanogens was suggested to occupy a primary pathway (approximately 95 to 99%) in total methanogenesis at the high dilution rate. Phylogenetic analyses of transcripts of the methyl coenzyme M reductase gene (mcrA) confirmed that a significant number of transcripts of the genera Methanoculleus (hydrogenotrophic methanogens) and Methanosarcina (aceticlastic methanogens) were present in the chemostats at the low and high dilution rates, respectively. The mcrA transcripts of the genus Methanosaeta (aceticlastic methanogens), which dominated the population in a previous study (T. Shigematsu, Y. Tang, H. Kawaguchi, K. Ninomiya, J. Kijima, T. Kobayashi, S. Morimura, and K. Kida, J. Biosci. Bioeng. 96:547-558, 2003), were poorly detected at both dilution rates due to the limited coverage of the primers used. These results demonstrated that the dilution rate could cause a shift in the primary pathway of acetate conversion to methane in acetate-fed chemostats.

Stable production of platform chemicals from the continuous hydrodeoxygenation of a raw bio-oil using enhanced carbon-based catalysts

2020 ◽  
Author(s):  
Idoia Hita ◽  
Tomas Cordero-Lanzac ◽  
Francisco J. Garcia-Mateos ◽  
Jose Rodriguez-Mirasol ◽  
Tomas Cordero ◽  
...  
Keyword(s):  
Platform Chemicals ◽  
Stable Production ◽  
Bio Oil ◽  
Carbon Based

Stable production of platform chemicals from the continuous hydrodeoxygenation of a raw bio-oil using enhanced carbon-based catalysts

2020 ◽  
Author(s):  
Idoia Hita ◽  
Tomas Cordero-Lanzac ◽  
Francisco J. Garcia-Mateos ◽  
Jose Rodriguez-Mirasol ◽  
Tomas Cordero ◽  
...  
Keyword(s):  
Platform Chemicals ◽  
Stable Production ◽  
Bio Oil ◽  
Carbon Based
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