On-line monitoring ofPhaffia rhodozyma fed-batch process with in situ dispersive raman spectroscopy

2003 ◽  
Vol 83 (6) ◽  
pp. 668-680 ◽  
Author(s):  
Christopher Cannizzaro ◽  
Martin Rhiel ◽  
Ian Marison ◽  
Urs von Stockar
Keyword(s):  
Raman Spectroscopy ◽  
Batch Process ◽  
Fed Batch ◽  
On Line

On-Line Monitoring of Airborne Chemistry in Levitated Nanodroplets:  In Situ Synthesis and Application of SERS-Active Ag−Sols for Trace Analysis by FT-Raman Spectroscopy

2003 ◽  
Vol 75 (9) ◽  
pp. 2166-2171 ◽  
Author(s):  
Nicolae Leopold ◽  
Michael Haberkorn ◽  
Thomas Laurell ◽  
Johan Nilsson ◽  
Josefa R. Baena ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Trace Analysis ◽  
In Situ Synthesis ◽  
Ft Raman Spectroscopy ◽  
On Line ◽  
Ft Raman

scholarly journals Simultaneous Infrared Spectroscopy, Raman Spectroscopy and Luminescence Sensing: A Multi-Spectroscopic Analyti-cal Platform

2021 ◽  
Author(s):  
Sarah Klingler ◽  
Julian Hniopek ◽  
Robert Stach ◽  
Michael Schmitt ◽  
Jürgen Popp ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Environmental Chemistry ◽  
Attenuated Total Reflection ◽  
Liquid Volume ◽  
On Line ◽  
Atr Spectroscopy ◽  
Gas Tight ◽  
Scientific Questions ◽  
Minute Sample

Scientific questions in fields such as catalysis, monitoring of biological processes or environmental chemistry demand for analytical technologies combining orthogonal spectroscopies. Combined spectroscopic concepts facilitate in-situ on-line monitoring of dynamic processes providing for a better understanding of the involved reaction pathways. In the present study, a low-liquid-volume multi-spectroscopic platform was developed based on infrared attenuated total reflection (IR-ATR) spectroscopy combined with Raman spectroscopy and lumines-cence sensing. For demonstrating the measurement capabilities, exemplary analyte systems including water / heavy water and aqueous solutions of ammonium sulfate were analyzed as proof-of-principle studies. It was successfully demonstrated that three optical techniques may be integrated into a single analytical platform with-out interference providing synchronized and complementary datasets by probing the same minute sample vol-ume. In addition, the developed assembly provides a gas-tight lid sealing the headspace above the probed liq-uid for monitoring the concentration of molecular oxygen also in the gas phase via luminescence quenching. Hence, the entire assembly may be operated at inert conditions, as required for example during the analysis of photocatalytic processes.

Raman Investigation of Photopolymerization Reactions of Single Optically Levitated Microparticles

1996 ◽  
Vol 50 (7) ◽  
pp. 823-828 ◽  
Author(s):  
C. Esen ◽  
T. Kaiser ◽  
G. Schweiger
Keyword(s):  
Raman Spectroscopy ◽  
Raman Spectrum ◽  
Raman Scattering ◽  
Polymerization Reaction ◽  
Single Particles ◽  
On Line ◽  
Optical Levitation ◽  
Monomer Droplet ◽  
Arc Excitation

Raman spectroscopy was used to investigate polymerization reactions in a single micrometer-sized monomer droplet. An Ar+ laser levitated the microparticles and simultaneously excited the Raman scattering. The polymerization reaction was initiated by exposing the monomer droplets to the UV radiation of a mercury arc excitation lamp. The Raman spectrum of the reacting particle was investigated on-line. The results demonstrate that the combination of the technique of optical levitation and Raman spectroscopy allows nondestructive in situ measurements of single particles and is therefore very useful for the study of fundamental processes.

scholarly journals NMPC-Based Workflow for Simultaneous Process and Model Development Applied to a Fed-Batch Process for Recombinant C. glutamicum

Processes ◽  
2020 ◽  
Vol 8 (10) ◽  
pp. 1313
Author(s):  
Philipp Levermann ◽  
Fabian Freiberger ◽  
Uma Katha ◽  
Henning Zaun ◽  
Johannes Möller ◽  
...  
Keyword(s):  
Process Development ◽  
Model Development ◽  
Target Function ◽  
Biomass Concentration ◽  
Product Yield ◽  
Batch Process ◽  
Fed Batch ◽  
Development Tool ◽  
On Line ◽  
Biotechnological Processes

For the fast and improved development of bioprocesses, new strategies are required where both strain and process development are performed in parallel. Here, a workflow based on a Nonlinear Model Predictive Control (NMPC) algorithm is described for the model-assisted development of biotechnological processes. By using the NMPC algorithm, the process is designed with respect to a target function (product yield, biomass concentration) with a drastically decreased number of experiments. A workflow for the usage of the NMPC algorithm as a process development tool is outlined. The NMPC algorithm is capable of improving various process states, such as product yield and biomass concentration. It uses on-line and at-line data and controls and optimizes the process by model-based process extrapolation. In this study, the algorithm is applied to a Corynebacterium glutamicum process. In conclusion, the potency of the NMPC algorithm as a powerful tool for process development is demonstrated. In particular, the benefits of the system regarding the characterization and optimization of a fed-batch process are outlined. With the NMPC algorithm, process development can be run simultaneously to strain development, resulting in a shortened time to market for novel products.

On-Line In-Situ Diagnostics of Processes Within PEM Fuel Cells by Raman Spectroscopy

2010 ◽  
Author(s):  
Hans Bettermann ◽  
Peter Fischer
Keyword(s):  
Raman Spectroscopy ◽  
Fuel Cells ◽  
Liquid Water ◽  
Water Distribution ◽  
Bipolar Plate ◽  
Pem Fuel Cells ◽  
Cell Processes ◽  
On Line ◽  
The Look

This contribution intends to show how Raman spectroscopy can be used to pursue processes inside PEM fuel cells. For this, two experimental setups have been worked out. At first a microscope objective was inserted into one bipolar plate. This enabled the detection of hydrogen, oxygen, nitrogen, vaporous and liquid water as well as changes in gas compositions and water distribution induced by an artificial pinhole within the MEA. A multiple-fiber Raman spectrometer with seven twin fibers was then developed to refine the look inside fuel cells. This setup permits the simultaneous record of gas compositions, liquid water and water vapor and how those species do change locally during operation. Beside the collection of data for modelling fuel cell processes, the multiple-fiber setup is primarily considered to monitor degradation processes and pre-stages of membrane damages.

Remote On-Line Control of a High-Voltage in situ Transmission Electron Microscope with A Rational User Interface

1996 ◽  
Vol 54 ◽  
pp. 384-385
Author(s):  
M.A. O’Keefe ◽  
J. Taylor ◽  
D. Owen ◽  
B. Crowley ◽  
K.H. Westmacott ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
User Interface ◽  
Transmission Electron Microscope ◽  
High Voltage ◽  
Materials Science ◽  
Transmission Electron ◽  
On Line ◽  
Electron Microscopes

Remote on-line electron microscopy is rapidly becoming more available as improvements continue to be developed in the software and hardware of interfaces and networks. Scanning electron microscopes have been driven remotely across both wide and local area networks. Initial implementations with transmission electron microscopes have targeted unique facilities like an advanced analytical electron microscope, a biological 3-D IVEM and a HVEM capable of in situ materials science applications. As implementations of on-line transmission electron microscopy become more widespread, it is essential that suitable standards be developed and followed. Two such standards have been proposed for a high-level protocol language for on-line access, and we have proposed a rational graphical user interface. The user interface we present here is based on experience gained with a full-function materials science application providing users of the National Center for Electron Microscopy with remote on-line access to a 1.5MeV Kratos EM-1500 in situ high-voltage transmission electron microscope via existing wide area networks. We have developed and implemented, and are continuing to refine, a set of tools, protocols, and interfaces to run the Kratos EM-1500 on-line for collaborative research. Computer tools for capturing and manipulating real-time video signals are integrated into a standardized user interface that may be used for remote access to any transmission electron microscope equipped with a suitable control computer.

Production of Rhamnolipid Biosurfactant from Fed Batch Culture by Pseudomonas aeruginosa using Multiple Substrates

2020 ◽  
Vol 16 (6) ◽  
pp. 928-933
Author(s):  
Jujjavarapu S. Eswari
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Batch Process ◽  
Time Interval ◽  
Fed Batch ◽  
Multiple Substrates ◽  
Surface Active Agents ◽  
Limiting Nutrients ◽  
Limiting Nutrient ◽  
Surface Active ◽  
Rhamnolipid Biosurfactant

Objective: Biosurfactants are the surface active agents which are used for the reduction of surface and interfacial tensions of liquids. Rhamnolipids are the surfactants produced by Pseudomonas aeruginosa. It requires minimum nutrition for its growth as it can also grow in distilled water. The rhamnolipids produced by Pseudomonas aeruginosa are extra-cellular glycolipids consisting of L-rhamnose and 3-hydroxyalkanoic acid. Methods: The fed-batch method for the rhamnolipid production is considered in this study to know the influence of the carbon, nitrogen, phosphorous substrates as growth-limiting nutrients. Pulse feeding is employed for limiting nutrient addition at particular time interval to obtain maximum rhamnolipid formation from Pseudomonas aeruginosa compared with the batch process. Results: Out of 3 fed batch strategies constant glucose fed batch strategy shows best and gave maximum rhamnolipid concentration of 0.134 g/l.

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