Fractional precipitation of plasmid DNA from lysate by CTAB

2002 ◽  
Vol 79 (7) ◽  
pp. 776-784 ◽  
Author(s):  
Russel J. Lander ◽  
Michael A. Winters ◽  
Francis J. Meacle ◽  
Barry C. Buckland ◽  
Ann L. Lee
Keyword(s):  
Plasmid Dna ◽  
Fractional Precipitation

scholarly journals Simple, inexpensive and RNase-free purification of plasmid DNA by fractional precipitation with isopropanol

BioTechniques ◽  
2021 ◽  
Author(s):  
Viiu Paalme ◽  
Mart Speek
Keyword(s):  
Quality Control ◽  
Column Chromatography ◽  
Plasmid Dna ◽  
Fractional Precipitation ◽  
Alkaline Lysis ◽  
Bacterial Extract ◽  
Total Rna ◽  
Spin Column ◽  
Commercial Kits

We present a modified alkaline lysis method for purification of plasmid DNA (pDNA) from bacterial extract using fractional precipitation with isopropanol (FPI). This method includes two successive precipitations with 0.33 and 0.36 volumes of isopropanol and separates pDNA from total RNA and most of the lipopolysaccharides. Using different quality control tests, we demonstrate that plasmids purified with FPI show superior quality compared to plasmids prepared with commercial kits based on spin-column chromatography.

Evaluation of biocompatibility and efficiency of plasmid DNA delivery by gene-activated hydrogels in vitro

2019 ◽  
Vol XIV (2) ◽  
Author(s):  
I.Y. Bozo ◽  
A.A. Titova ◽  
M.N. Zhuravleva ◽  
A.I. Bilyalov ◽  
M.O. Mavlikeev ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery

Detection of Rhizoctonia solani AG-2-2 IV, the Causal Agent of Large Patch of Zoysiagrass, Using Plasmid DNA as a Probe.

1998 ◽  
Vol 64 (5) ◽  
pp. 451-457 ◽  
Author(s):  
Susumu TAKAMATSU ◽  
Manami NAKANO ◽  
Hideyuki YOKOTA ◽  
Hitoshi KUNOH
Keyword(s):  
Rhizoctonia Solani ◽  
Plasmid Dna ◽  
Causal Agent ◽  
Large Patch
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