Development of a novel nanoparticle by dual modification with the pluripotential cell-penetrating peptide PepFect6 for cellular uptake, endosomal escape, and decondensation of an siRNA core complex

Biopolymers ◽  
2013 ◽  
Vol 100 (6) ◽  
pp. 698-704 ◽  
Author(s):  
Asako Mitsueda ◽  
Yuri Shimatani ◽  
Masahiro Ito ◽  
Takashi Ohgita ◽  
Asako Yamada ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Endosomal Escape ◽  
Cell Penetrating Peptide ◽  
Core Complex ◽  
Cell Penetrating

scholarly journals Cooperative Cellular Uptake and Activity of Octaarginine Antisense Peptide Nucleic acid (PNA) Conjugates

Biomolecules ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 554 ◽  
Author(s):  
Ghavami ◽  
Shiraishi ◽  
Nielsen
Keyword(s):  
Cellular Uptake ◽  
Peptide Nucleic Acid ◽  
Peptide Nucleic Acids ◽  
Endosomal Escape ◽  
Cell Penetrating Peptide ◽  
Uptake Mechanism ◽  
Common Effect ◽  
Cell Penetrating ◽  
Antisense Peptide ◽  
Endosomal Uptake

Cellular uptake and antisense activity of d-octaarginine conjugated peptide nucleic acids (PNAs) is shown to exhibit pronounced cooperativity in serum-containing medium, in particular by being enhanced by analogous mis-match PNA–cell-penetrating peptide (PNA–CPP) conjugates without inherent antisense activity. This cooperativity does not show cell or PNA sequence dependency, suggesting that it is a common effect in cationic CPP conjugated PNA delivery. Interestingly, our results also indicate that Deca-r8-PNA and r8-PNA could assist each other and even other non-CPP PNAs as an uptake enhancer agent. However, the peptide itself (without being attached to the PNA) failed to enhance uptake and antisense activity. These results are compatible with an endosomal uptake mechanism in which the endocytosis event is induced by multiple CPP–PNA binding to the cell surface requiring a certain CPP density, possibly in terms of nanoparticle number and/or size, to be triggered. In particular the finding that the number of endosomal events is dependent on the total CPP–PNA concentration supports such a model. It is not possible from the present results to conclude whether endosomal escape is also cooperatively induced by CPP–PNA.

scholarly journals A comparison of acyl-moieties for noncovalent functionalization of PLGA and PEG-PLGA nanoparticles with a cell-penetrating peptide

RSC Advances ◽  
2021 ◽  
Vol 11 (57) ◽  
pp. 36116-36124
Author(s):  
Omar Paulino da Silva Filho ◽  
Muhanad Ali ◽  
Rike Nabbefeld ◽  
Daniel Primavessy ◽  
Petra H. Bovee-Geurts ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Plga Nanoparticles ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating Peptide ◽  
Noncovalent Functionalization ◽  
Cell Penetrating ◽  
A Cell

Noncovalent functionalization with acylated cell-penetrating peptides achieves an efficient cellular uptake of PLGA and PEG-PLGA nanoparticles.

Lipid–Cell-Penetrating-Peptide Conjugates as Effective Delivery Systems

Synlett ◽  
2017 ◽  
Vol 28 (15) ◽  
pp. 1897-1900
Author(s):  
Zi-Gang Li ◽  
Yan-Hong Jiang ◽  
Hui Zhao ◽  
Yuan Tian
Keyword(s):  
Cellular Uptake ◽  
Biological Research ◽  
Cell Penetrating Peptide ◽  
Peptide Conjugates ◽  
Cellular Toxicity ◽  
Wide Range ◽  
Cell Penetrating ◽  
Effective Delivery ◽  
Cellular Permeability ◽  
Small Molecule Therapeutics

A lipid–cell-penetrating-peptide (CPP) conjugate was designed to deliver cargoes with poor cellular permeability, including peptides or small-molecule therapeutics, into cells. The lipid–CPP conjugate facilitated the cellular uptake of cargoes noncovalently through an ATP-dependent micropinocytosis mechanism. This delivery system is simple, efficient, has minimal cellular toxicity, and might be useful in a wide range of biological research.

gH625 Cell-Penetrating Peptide Promotes the Endosomal Escape of Nanovectorized siRNA in a Triple-Negative Breast Cancer Cell Line

2019 ◽  
Vol 20 (8) ◽  
pp. 3076-3086 ◽  
Author(s):  
Sanaa Ben Djemaa ◽  
Katel Hervé-Aubert ◽  
Laurie Lajoie ◽  
Annarita Falanga ◽  
Stefania Galdiero ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Breast Cancer Cell ◽  
Triple Negative Breast Cancer ◽  
Breast Cancer Cell Line ◽  
Triple Negative ◽  
Cancer Cell Line ◽  
Endosomal Escape ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating

The influence of cell penetrating peptide branching on cellular uptake of QDs

2016 ◽  
Author(s):  
Joyce Breger ◽  
James Delehanty ◽  
Kimihiro Susumu ◽  
George Anderson ◽  
Markus Muttenhaler ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating
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