Peptides as models for the structure and function of viral capsid proteins: Insights on dengue virus capsid

Biopolymers ◽  
2013 ◽  
Vol 100 (4) ◽  
pp. 325-336 ◽  
Author(s):  
João Miguel Freire ◽  
Ana Salomé Veiga ◽  
Beatriz G. de la Torre ◽  
Nuno C. Santos ◽  
David Andreu ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Structure And Function ◽  
Capsid Proteins ◽  
Viral Capsid ◽  
Virus Capsid ◽  
And Function ◽  
Viral Capsid Proteins

scholarly journals Adenovirus Core Proteins: Structure and Function

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 388
Author(s):  
Shermila Kulanayake ◽  
Suresh K. Tikoo
Keyword(s):  
Rna Splicing ◽  
Viral Vectors ◽  
Structure And Function ◽  
Viral Capsid ◽  
Cellular Processes ◽  
Core Proteins ◽  
The Subject ◽  
And Function ◽  
Viral Capsid Proteins

Adenoviruses have served as a model for investigating viral-cell interactions and discovering different cellular processes, such as RNA splicing and DNA replication. In addition, the development and evaluation of adenoviruses as the viral vectors for vaccination and gene therapy has led to detailed investigations about adenovirus biology, including the structure and function of the adenovirus encoded proteins. While the determination of the structure and function of the viral capsid proteins in adenovirus biology has been the subject of numerous reports, the last few years have seen increased interest in elucidating the structure and function of the adenovirus core proteins. Here, we provide a review of research about the structure and function of the adenovirus core proteins in adenovirus biology.

Structure and Function of the Non-Structural Protein of Dengue Virus and its Applications in Antiviral Therapy

2016 ◽  
Vol 17 (3) ◽  
pp. 371-380 ◽  
Author(s):  
Qian Xie ◽  
Bao Zhang ◽  
JianHai Yu ◽  
Qinghua Wu ◽  
Fangji Yang ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Antiviral Therapy ◽  
Structure And Function ◽  
Structural Protein ◽  
And Function

scholarly journals Nonstructural Protein NP1 of Human Bocavirus 1 Plays a Critical Role in the Expression of Viral Capsid Proteins

2016 ◽  
Vol 90 (9) ◽  
pp. 4658-4669 ◽  
Author(s):  
Wei Zou ◽  
Fang Cheng ◽  
Weiran Shen ◽  
John F. Engelhardt ◽  
Ziying Yan ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Capsid Protein ◽  
Nonstructural Protein ◽  
Critical Role ◽  
The Other ◽  
Capsid Proteins ◽  
Viral Capsid ◽  
Human Bocavirus ◽  
Human Airway ◽  
Viral Capsid Proteins

ABSTRACTA novel chimeric parvoviral vector, rAAV2/HBoV1, in which the recombinant adeno-associated virus 2 (rAAV2) genome is pseudopackaged by the human bocavirus 1 (HBoV1) capsid, has been shown to be highly efficient in gene delivery to human airway epithelia (Z. Yan et al., Mol Ther 21:2181–2194, 2013,http://dx.doi.org/10.1038/mt.2013.92). In this vector production system, we used an HBoV1 packaging plasmid, pHBoV1NSCap, that harbors HBoV1 nonstructural protein (NS) and capsid protein (Cap) genes. In order to simplify this packaging plasmid, we investigated the involvement of the HBoV1 NS proteins in capsid protein expression. We found that NP1, a small NS protein encoded by the middle open reading frame, is required for the expression of the viral capsid proteins (VP1, VP2, and VP3). We also found that the other NS proteins (NS1, NS2, NS3, and NS4) are not required for the expression of VP proteins. We performed systematic analyses of the HBoV1 mRNAs transcribed from the pHBoV1NSCap packaging plasmid and its derivatives in HEK 293 cells. Mechanistically, we found that NP1 is required for both the splicing and the read-through of the proximal polyadenylation site of the HBoV1 precursor mRNA, essential functions for the maturation of capsid protein-encoding mRNA. Thus, our study provides a unique example of how a small viral nonstructural protein facilitates the multifaceted regulation of capsid gene expression.IMPORTANCEA novel chimeric parvoviral vector, rAAV2/HBoV1, expressing a full-length cystic fibrosis transmembrane conductance regulator (CFTR) gene, is capable of correcting CFTR-dependent chloride transport in cystic fibrosis human airway epithelium. Previously, an HBoV1 nonstructural and capsid protein-expressing plasmid, pHBoV1NSCap, was used to package the rAAV2/HBoV1 vector, but yields remained low. In this study, we demonstrated that the nonstructural protein NP1 is required for the expression of capsid proteins. However, we found that the other four nonstructural proteins (NS1 to -4) are not required for expression of capsid proteins. By mutating theciselements that function as internal polyadenylation signals in the capsid protein-expressing mRNA, we constructed a simple HBoV1 capsid protein-expressing gene that expresses capsid proteins as efficiently as pHBoV1NSCap does, and at similar ratios, but independently of NP1. Our study provides a foundation to develop a better packaging system for rAAV2/HBoV1 vector production.

scholarly journals In Vitro Infection with Dengue Virus Induces Changes in the Structure and Function of the Mouse Brain Endothelium

PLoS ONE ◽  
2016 ◽  
Vol 11 (6) ◽  
pp. e0157786 ◽  
Author(s):  
Myriam L. Velandia-Romero ◽  
María-Angélica Calderón-Peláez ◽  
Jaime E. Castellanos
Keyword(s):  
Dengue Virus ◽  
Mouse Brain ◽  
Structure And Function ◽  
Brain Endothelium ◽  
And Function
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