Raman and surface enhanced Raman spectroscopy of 2,2,5,5-tetramethyl-3-pyrrolin-1-yloxy-3-carboxamide labeled proteins: Bovine serum albumin and cytochromec

Biopolymers ◽  
2001 ◽  
Vol 62 (6) ◽  
pp. 341-348 ◽  
Author(s):  
S. Cavalu ◽  
S. C�nt?-P�nzaru ◽  
N. Leopold ◽  
W. Kiefer
Keyword(s):  
Raman Spectroscopy ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Monodispersed Protein Stabilized Silver Nanoprisms: Synthesis, Optical Properties and Surface-Enhanced Raman Scattering Application

2011 ◽  
Vol 688 ◽  
pp. 162-167 ◽  
Author(s):  
Dan Hui Zhang ◽  
Xiao Heng Liu ◽  
Xin Wang
Keyword(s):  
Bovine Serum Albumin ◽  
Raman Scattering ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Surface Enhanced Raman Scattering ◽  
Water Soluble ◽  
Silver Nanoprisms ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Protein stabilized, water soluble silver nanoparticles are essential for biomedicine and biotechnology. The abundant protein such as gelatin and bovine serum albumin (BSA) has a remarkable ability of binding and transporting materials across cell membrane that makes it ideal for drug delivery and very useful for pharmaceutical industry. In this article, silver nanoprisms were synthesized by reacting an aqueous solution of silver nitrate with proteins such as gelatin and bovine serum albumin at different conditions, where proteins provided the dual function of Ag (I) reduction and Ag(0) prism-like structures formation. The solution exhibited an excellent colloidal stability by the visible observation. UV-vis spectra and transmission electron microscopy (TEM), revealed the formation of well-dispersed Ag nanoprisms with different sizes. Furthermore, the fluorescence intensity of proteins was drastically quenched in presence of Ag nanoprisms from the results of fluorescence spectra. The surface enhanced Raman scattering spectrum of anisotropic silver nanoprisms was also presented.

Surface-Enhanced Raman Scattering (SERS) Spectroscopy with Borohydride-Reduced Silver Colloids: Controlling Adsorption of the Scattering Species by Surface Potential of Silver Colloid

1993 ◽  
Vol 58 (11) ◽  
pp. 2682-2694 ◽  
Author(s):  
Kateřina Čermáková ◽  
Ondřej Šesták ◽  
Pavel Matějka ◽  
Vladimír Baumruk ◽  
Blanka Vlčková
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Surface Potential ◽  
Bovine Serum ◽  
Colloidal Particles ◽  
Molar Ratio ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Ag Colloid

Formation of Ag colloid/adsorbate SERS-active systems (upon adsorption of the selected adsorbates on the surface of Ag colloidal particles) as a function of (i) NaBH4 to AgNO3 molar ratio in the preparation protocol of Ag colloid, and (ii) aging of the colloid has been investigated by Surface-enhanced Raman scattering (SERS) spectroscopy. Oligomeric synthetic polypeptides, bovine serum albumin, phosphate coadsorbed with CuTMePyP [copper(II) derivative of 5,10,15,20-tetrakis-(N-methylpyridinium-4-yl)porphyrin chloride] and borates in systems with N-containing bases were selected as model adsorbates. Both (i) a decrease of NaBH4 to AgNO3 molar ratio upon preparation and (ii) aging of Ag colloid affect adsorption of the adsorbates and consequently, their SERS spectra, in the same manner. Aging of Ag colloid is thus viewed as a slow hydrolysis of BH4- anions. The actual concentration of BH4- in the system is identified as the most important factor controlling adsorption of all the selected adsorbates on the surface of Ag colloid. As this factor can be related to the surface potential, the conditions controlling adsorption of the selected adsorbates are specified in terms of a more negative and/or more positive surface potential of Ag colloidal particles. A more positive surface potential promotes adsorption of polypeptides, bovine serum albumin and phosphate while observation of spectral features of borates in the SERS spectra of N-containing bases in alkaline solutions is conditioned by a more negative surface potential.

Detection of trace mercury ions in water with a bovine-serum-albumin-modified Au@SiNWAs surface-enhanced-Raman-scattering sensor

2021 ◽  
Author(s):  
Chengshan Fan ◽  
Jiameng Lv ◽  
Hai Jun Xu ◽  
Jiahao Bai ◽  
Longtao Ren ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Raman Scattering ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Silicon Nanowire ◽  
Surface Enhanced Raman Scattering ◽  
Mercury Ions ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Accurate detection of mercury ions (Hg2+) in water is of great importance for environmental protection. Here, a surface-enhanced Raman scattering (SERS) sensor using bovine-serum-albumin-modified gold-silicon nanowire arrays (Au@SiNWAs) is used...

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