Investigation of the binding sites and orientation of Norfloxacin on bovine serum albumin by surface enhanced Raman scattering and molecular docking

2019 ◽  
Vol 207 ◽  
pp. 307-312 ◽  
Author(s):  
Wenhui Lian ◽  
Yawen Liu ◽  
Hongmei Yang ◽  
Hao Ma ◽  
Rui Su ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Bovine Serum Albumin ◽  
Raman Scattering ◽  
Serum Albumin ◽  
Binding Sites ◽  
Bovine Serum ◽  
Surface Enhanced Raman Scattering ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Monodispersed Protein Stabilized Silver Nanoprisms: Synthesis, Optical Properties and Surface-Enhanced Raman Scattering Application

2011 ◽  
Vol 688 ◽  
pp. 162-167 ◽  
Author(s):  
Dan Hui Zhang ◽  
Xiao Heng Liu ◽  
Xin Wang
Keyword(s):  
Bovine Serum Albumin ◽  
Raman Scattering ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Surface Enhanced Raman Scattering ◽  
Water Soluble ◽  
Silver Nanoprisms ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Protein stabilized, water soluble silver nanoparticles are essential for biomedicine and biotechnology. The abundant protein such as gelatin and bovine serum albumin (BSA) has a remarkable ability of binding and transporting materials across cell membrane that makes it ideal for drug delivery and very useful for pharmaceutical industry. In this article, silver nanoprisms were synthesized by reacting an aqueous solution of silver nitrate with proteins such as gelatin and bovine serum albumin at different conditions, where proteins provided the dual function of Ag (I) reduction and Ag(0) prism-like structures formation. The solution exhibited an excellent colloidal stability by the visible observation. UV-vis spectra and transmission electron microscopy (TEM), revealed the formation of well-dispersed Ag nanoprisms with different sizes. Furthermore, the fluorescence intensity of proteins was drastically quenched in presence of Ag nanoprisms from the results of fluorescence spectra. The surface enhanced Raman scattering spectrum of anisotropic silver nanoprisms was also presented.

Detection of trace mercury ions in water with a bovine-serum-albumin-modified Au@SiNWAs surface-enhanced-Raman-scattering sensor

2021 ◽  
Author(s):  
Chengshan Fan ◽  
Jiameng Lv ◽  
Hai Jun Xu ◽  
Jiahao Bai ◽  
Longtao Ren ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Raman Scattering ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Silicon Nanowire ◽  
Surface Enhanced Raman Scattering ◽  
Mercury Ions ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Accurate detection of mercury ions (Hg2+) in water is of great importance for environmental protection. Here, a surface-enhanced Raman scattering (SERS) sensor using bovine-serum-albumin-modified gold-silicon nanowire arrays (Au@SiNWAs) is used...

scholarly journals Surface-enhanced Raman scattering investigation of bovine serum albumin by Au nanoparticles with different sizes

2018 ◽  
Vol 16 (1_suppl) ◽  
pp. 157-162 ◽  
Author(s):  
Wei Xiaodan ◽  
Zheng Dawei ◽  
Zhang Ping ◽  
Lin Taifeng ◽  
Wang Huiqin ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Raman Scattering ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Au Nanoparticles ◽  
Structural Information ◽  
Biological Systems ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Background: Surface-enhanced Raman scattering (SERS) has become a useful spectroscopic tool for studying biomolecule structures. The main types of plasmonic substrates used in biological systems are Au nanoparticles (AuNPs), whose surface plasmon resonance depends on the nanoparticle size, morphology, particle interspace, and so on. Methods: In this study, AuNP colloids with different sizes were synthesized and used as the sensors to probe SERS signals of different biomarkers and biomolecules. Results: The results showed that an AuNP colloid of ~50 nm had excellent SERS effects in probing various molecules, and could be preserved for about 3 months with excellent repeatability and reproducibility (RSD <5%) in terms of the probed signal intensity (rhodamine 6G and crystal violet). Meanwhile, the fabricated AuNPs were applied to study the SERS signals and structural information of bovine serum albumin (BSA) in aqueous solution. It was found that SERS could rapidly provide the structural information and vibration characteristics of BSA. Conclusion: It was concluded that biocompatible AuNP colloid may be a promising biosensor in the rapid and label-free detection of biological systems.

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