Site-Specific Photoconjugation of Beta-Lactamase Fragments to Monoclonal Antibodies Enables Sensitive Analyte Detection via Split-Enzyme Complementation

2018 ◽  
Vol 13 (7) ◽  
pp. 1700688 ◽  
Author(s):  
Feifan Yu ◽  
Veronica Alesand ◽  
Per-Åke Nygren
Keyword(s):  
Monoclonal Antibodies ◽  
Beta Lactamase ◽  
Site Specific ◽  
Analyte Detection

High Yield, Site-Specific Coupling of N-Terminally Modified .beta.-Lactamase to a Proteolytically Derived Single-Sulfhydryl Murine Fab'

1994 ◽  
Vol 5 (6) ◽  
pp. 636-646 ◽  
Author(s):  
Stephen D. Mikolajczyk ◽  
Damon L. Meyer ◽  
James J. Starling ◽  
Kevin L. Law ◽  
Keith Rose ◽  
...  
Keyword(s):  
High Yield ◽  
Beta Lactamase ◽  
Site Specific

Discrimination of rat Brunner's gland carbohydrate antigens by site-specific monoclonal antibodies

2016 ◽  
Vol 432 ◽  
pp. 76-82 ◽  
Author(s):  
Tomoyuki Chimuro ◽  
Hiroyuki Kuroyama ◽  
Yukinobu Goso ◽  
Kazuhiko Ishihara ◽  
Makoto Kurihara
Keyword(s):  
Monoclonal Antibodies ◽  
Carbohydrate Antigens ◽  
Site Specific ◽  
Brunner’S Gland ◽  
Brunner's Gland

Site-Specific Immobilization of Monoclonal Antibodies Using Spacer-Mediated Antibody Attachment

Langmuir ◽  
1996 ◽  
Vol 12 (17) ◽  
pp. 4292-4298 ◽  
Author(s):  
Shao-Chie (Patrick) Huang ◽  
Karin D. Caldwell ◽  
Jinn-Nan Lin ◽  
Hsu-Kun Wang ◽  
James N. Herron
Keyword(s):  
Monoclonal Antibodies ◽  
Site Specific

IRBP: preparation and characterization of site-specific monoclonal antibodies

1990 ◽  
Vol 9 (4) ◽  
pp. 357-362 ◽  
Author(s):  
Larry A. Donoso ◽  
Merlyn Rodrigues ◽  
Tamara R. Vrabec ◽  
Theodore W. Sery ◽  
Shao-Ling Fong
Keyword(s):  
Monoclonal Antibodies ◽  
Site Specific

Production of Monoclonal Antibodies with Site-Specific Reactivity in Human Colonic Mucosa

1994 ◽  
Vol 86 (s30) ◽  
pp. 11P-11P ◽  
Author(s):  
JE Smithson ◽  
C Prince ◽  
R Pigott ◽  
DP Jewell
Keyword(s):  
Monoclonal Antibodies ◽  
Colonic Mucosa ◽  
Site Specific ◽  
Specific Reactivity

Detection of the dystroglycanopathy protein, fukutin, using a new panel of site-specific monoclonal antibodies

2012 ◽  
Vol 424 (2) ◽  
pp. 354-357 ◽  
Author(s):  
Tracy A. Lynch ◽  
Le Thanh Lam ◽  
Nguyen thi Man ◽  
Kazuhiro Kobayashi ◽  
Tatsushi Toda ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Site Specific

scholarly journals Site-specific inhibition of myosin-mediated motility in vitro by monoclonal antibodies.

1985 ◽  
Vol 100 (4) ◽  
pp. 1024-1030 ◽  
Author(s):  
P F Flicker ◽  
G Peltz ◽  
M P Sheetz ◽  
P Parham ◽  
J A Spudich
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibodies ◽  
Binding Sites ◽  
Functional Assay ◽  
Specific Inhibition ◽  
Myosin Molecule ◽  
Site Specific ◽  
Dictyostelium Myosin ◽  
Cell Biol

Monoclonal antibodies directed against seven different sites on Dictyostelium myosin (Peltz, G., J. A. Spudich, and P. Parham, 1985, J. Cell Biol., 100: 1016-1023) were tested for their ability to inhibit movement of myosin in vitro, using the Nitella-based myosin-mediated bead movement assay (Sheetz, M. P., R. Chasan, and J. A. Spudich, 1984, J. Cell Biol., 99: 1867-1871). To complement this functional assay, we located the binding sites of these antibodies by electron microscopy, using the rotary shadowing technique. One antibody bound to the 18,000-dalton light chain and inhibited movement completely. All of the remaining antibodies bound to various positions along the rod portion of the myosin molecule, which is approximately 1,800 A long. Antibodies that bound to the rod about 470, 680, and 1400 A from the head-tail junction did not alter myosin movement. One antibody appeared to bind very close to the head-tail junction and to inhibit movement 50%. Surprisingly, three antibodies that bound about 1,200 A from the head-tail junction inhibited movement completely. This inhibition did not depend on using intact IgG, since Fab' fragments had the same effect.

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