Production of Monoclonal Antibodies with Site-Specific Reactivity in Human Colonic Mucosa

1994 ◽  
Vol 86 (s30) ◽  
pp. 11P-11P ◽  
Author(s):  
JE Smithson ◽  
C Prince ◽  
R Pigott ◽  
DP Jewell
Keyword(s):  
Monoclonal Antibodies ◽  
Colonic Mucosa ◽  
Site Specific ◽  
Specific Reactivity

Discrimination of rat Brunner's gland carbohydrate antigens by site-specific monoclonal antibodies

2016 ◽  
Vol 432 ◽  
pp. 76-82 ◽  
Author(s):  
Tomoyuki Chimuro ◽  
Hiroyuki Kuroyama ◽  
Yukinobu Goso ◽  
Kazuhiko Ishihara ◽  
Makoto Kurihara
Keyword(s):  
Monoclonal Antibodies ◽  
Carbohydrate Antigens ◽  
Site Specific ◽  
Brunner’S Gland ◽  
Brunner's Gland

Site-Specific Immobilization of Monoclonal Antibodies Using Spacer-Mediated Antibody Attachment

Langmuir ◽  
1996 ◽  
Vol 12 (17) ◽  
pp. 4292-4298 ◽  
Author(s):  
Shao-Chie (Patrick) Huang ◽  
Karin D. Caldwell ◽  
Jinn-Nan Lin ◽  
Hsu-Kun Wang ◽  
James N. Herron
Keyword(s):  
Monoclonal Antibodies ◽  
Site Specific

Evaluation of the Extrapineal Sources of Melatonin in Patients with Lymphocytic Colitis

2017 ◽  
Vol 6 (04) ◽  
Author(s):  
Cezary Chojnacki ◽  
Janusz Błasiak ◽  
Jakub Fichna ◽  
Jan Chojnacki ◽  
Tomasz Popławski ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Healthy Subjects ◽  
Chromogranin A ◽  
Colonic Mucosa ◽  
Intraepithelial Lymphocytes ◽  
Urine Concentration ◽  
Enterochromaffin Cells ◽  
Lymphocytic Colitis ◽  
Photometric Method ◽  
Rt Pcr

Melatonin can be synthesized by different cells in the gastrointestinal tract. The aim of this study was to evaluate the expression of melatonin-synthesizing enzymes: arylalkylamine-N-acetyltransferase(AANAT) and N-acetylserotonin methyltransferase (ASMT) in colonic mucosa and urinary 6-sulfatoxymelatonin excretion(aMT6s) in patients with lymphocytic colitis(LC) related to the number of intraepithelial lymphocytes(IEL) and enterochromaffin cells (EC). The study included 32 healthy subjects (HS group) and 36 patients with lymphocytic colitis (LC group). The diagnosis was based on histological and immunohistochemical performances using mouse monoclonal antibodies (chromogranin A –LK 2H10). EC were counted in 10 fields of each bioptate at 40x magnification. The level of mRNA expression of AANAT and ASMT was estimated in colonic mucosa with RT-PCR. The urine concentration of aMT6s was determined by photometric method. Significant differences were found in obtained results between HS and LC group: the number of IEL 14.1±3.41 vs. 32.4±5.50 per 100 surface colonocytes (p less than 0.001); the number of EC 32.2±10.1 vs. 70.4±21.2 (p less than 0.001); AANAT -1.32±0.47 vs. 2.32±0.65 (pless than 0.001; ASMT – 1.51±0.59 vs. 2.80±0.92 (p less than 0.001) aMT6s- 13.4±4.87 vs. 19.9±6.29 (p less than 0.001). Moreover, a positive correlation was found between the number of EC and ASMT (p= 0.011) and between EC and aMT6s (p less than 0.001). No correlation occurred between the number of IEL and AANAT p=0.932) and AMST (n=0.536) and aMT6s (p=9.288). These results indicate that the enterochromaffin cells in colonic mucosa are the main extrapineal source of melatonin in patients with lymphocytic colitis.

scholarly journals Site-Specific Reactivity of Nonenzymatic Lysine Acetylation

2015 ◽  
Vol 10 (1) ◽  
pp. 122-128 ◽  
Author(s):  
Josue Baeza ◽  
Michael J. Smallegan ◽  
John M. Denu
Keyword(s):  
Lysine Acetylation ◽  
Site Specific ◽  
Specific Reactivity

IRBP: preparation and characterization of site-specific monoclonal antibodies

1990 ◽  
Vol 9 (4) ◽  
pp. 357-362 ◽  
Author(s):  
Larry A. Donoso ◽  
Merlyn Rodrigues ◽  
Tamara R. Vrabec ◽  
Theodore W. Sery ◽  
Shao-Ling Fong
Keyword(s):  
Monoclonal Antibodies ◽  
Site Specific

Experimentally Determined Site-Specific Reactivity of the Gas-Phase OH and Cl + i-Butanol Reactions Between 251 and 340 K

2016 ◽  
Vol 120 (50) ◽  
pp. 9968-9981 ◽  
Author(s):  
Max R. McGillen ◽  
Geoffrey S. Tyndall ◽  
John J. Orlando ◽  
Andre S. Pimentel ◽  
Diogo J. Medeiros ◽  
...  
Keyword(s):  
Gas Phase ◽  
Site Specific ◽  
Specific Reactivity

Detection of the dystroglycanopathy protein, fukutin, using a new panel of site-specific monoclonal antibodies

2012 ◽  
Vol 424 (2) ◽  
pp. 354-357 ◽  
Author(s):  
Tracy A. Lynch ◽  
Le Thanh Lam ◽  
Nguyen thi Man ◽  
Kazuhiro Kobayashi ◽  
Tatsushi Toda ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Site Specific

scholarly journals Site-specific inhibition of myosin-mediated motility in vitro by monoclonal antibodies.

1985 ◽  
Vol 100 (4) ◽  
pp. 1024-1030 ◽  
Author(s):  
P F Flicker ◽  
G Peltz ◽  
M P Sheetz ◽  
P Parham ◽  
J A Spudich
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibodies ◽  
Binding Sites ◽  
Functional Assay ◽  
Specific Inhibition ◽  
Myosin Molecule ◽  
Site Specific ◽  
Dictyostelium Myosin ◽  
Cell Biol

Monoclonal antibodies directed against seven different sites on Dictyostelium myosin (Peltz, G., J. A. Spudich, and P. Parham, 1985, J. Cell Biol., 100: 1016-1023) were tested for their ability to inhibit movement of myosin in vitro, using the Nitella-based myosin-mediated bead movement assay (Sheetz, M. P., R. Chasan, and J. A. Spudich, 1984, J. Cell Biol., 99: 1867-1871). To complement this functional assay, we located the binding sites of these antibodies by electron microscopy, using the rotary shadowing technique. One antibody bound to the 18,000-dalton light chain and inhibited movement completely. All of the remaining antibodies bound to various positions along the rod portion of the myosin molecule, which is approximately 1,800 A long. Antibodies that bound to the rod about 470, 680, and 1400 A from the head-tail junction did not alter myosin movement. One antibody appeared to bind very close to the head-tail junction and to inhibit movement 50%. Surprisingly, three antibodies that bound about 1,200 A from the head-tail junction inhibited movement completely. This inhibition did not depend on using intact IgG, since Fab' fragments had the same effect.

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