13 C Flux Analysis Reveals that Rebalancing Medium Amino Acid Composition can Reduce Ammonia Production while Preserving Central Carbon Metabolism of CHO Cell Cultures

2018 ◽  
Vol 13 (10) ◽  
pp. 1700518 ◽  
Author(s):  
Allison G. McAtee Pereira ◽  
Jason L. Walther ◽  
Myles Hollenbach ◽  
Jamey D. Young
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Cell Cultures ◽  
Carbon Metabolism ◽  
Central Carbon Metabolism ◽  
Flux Analysis ◽  
Ammonia Production ◽  
Cho Cell ◽  
Central Carbon

Optimizing amino acid composition of CHO cell culture media for a fusion protein production

2011 ◽  
Vol 46 (7) ◽  
pp. 1423-1429 ◽  
Author(s):  
Zizhuo Xing ◽  
Brian Kenty ◽  
Inna Koyrakh ◽  
Michael Borys ◽  
Shih-Hsie Pan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Amino Acid ◽  
Fusion Protein ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Protein Production ◽  
Culture Media ◽  
Cho Cell ◽  
Cell Culture Media ◽  
Cho Cell Culture

scholarly journals Transcriptomic Analysis of the Influence of Methanol Assimilation on the Gene Expression in the Recombinant Pichia pastoris Producing Hirudin Variant 3

Genes ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 606 ◽  
Author(s):  
Li ◽  
Ma ◽  
Xu ◽  
Wang ◽  
Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Amino Acid ◽  
Pichia Pastoris ◽  
Carbon Metabolism ◽  
Fermentation Broth ◽  
Alcohol Oxidase ◽  
Central Carbon Metabolism ◽  
Recombinant Pichia Pastoris ◽  
Central Carbon ◽  
Almost All

Hirudin and its variants, as strong inhibitors against thrombin, are present in the saliva of leeches and are recognized as potent anticoagulants. However, their yield is far from the clinical requirement up to now. In this study, the production of hirudin variant 3 (HV3) was successfully realized by cultivating the recombinant Pichia pastoris GS115/pPIC9K-hv3 under the regulation of the promoter of AOX1 encoding alcohol oxidase (AOX). The antithrombin activity in the fermentation broth reached the maximum value of 5000 ATU/mL. To explore an effective strategy for improving HV3 production in the future, we investigated the influence of methanol assimilation on the general gene expression in this recombinant by transcriptomic study. The results showed that methanol was partially oxidized into CO2, and the rest was converted into glycerone-P which subsequently entered into central carbon metabolism, energy metabolism, and amino acid biosynthesis. However, the later metabolic processes were almost all down-regulated. Therefore, we propose that the up-regulated central carbon metabolism, energy, and amino acid metabolism should be beneficial for methanol assimilation, which would accordingly improve the production of HV3.

Metabolic flux analysis ofShewanellaspp. reveals evolutionary robustness in central carbon metabolism

2009 ◽  
Vol 102 (4) ◽  
pp. 1161-1169 ◽  
Author(s):  
Yinjie J. Tang ◽  
Hector Garcia Martin ◽  
Paramvir S. Dehal ◽  
Adam Deutschbauer ◽  
Xavier Llora ◽  
...  
Keyword(s):  
Carbon Metabolism ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Central Carbon Metabolism ◽  
Flux Analysis ◽  
Central Carbon ◽  
Evolutionary Robustness
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