Optimizing amino acid composition of CHO cell culture media for a fusion protein production

2011 ◽  
Vol 46 (7) ◽  
pp. 1423-1429 ◽  
Author(s):  
Zizhuo Xing ◽  
Brian Kenty ◽  
Inna Koyrakh ◽  
Michael Borys ◽  
Shih-Hsie Pan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Amino Acid ◽  
Fusion Protein ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Protein Production ◽  
Culture Media ◽  
Cho Cell ◽  
Cell Culture Media ◽  
Cho Cell Culture

scholarly journals Benchmarking of commercially available CHO cell culture media for antibody production

2015 ◽  
Vol 99 (11) ◽  
pp. 4645-4657 ◽  
Author(s):  
David Reinhart ◽  
Lukas Damjanovic ◽  
Christian Kaisermayer ◽  
Renate Kunert
Keyword(s):  
Cell Culture ◽  
Antibody Production ◽  
Culture Media ◽  
Cho Cell ◽  
Cell Culture Media ◽  
Cho Cell Culture

Elucidating the Impact of CHO Cell Culture Media on Tryptophan Oxidation of a Monoclonal Antibody Through Gene Expression Analyses

2018 ◽  
Vol 13 (10) ◽  
pp. 1700254 ◽  
Author(s):  
Luhong He ◽  
Jairav X. Desai ◽  
Jinxin Gao ◽  
Laurie B. Hazeltine ◽  
Zhirui Lian ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Culture ◽  
Monoclonal Antibody ◽  
Culture Media ◽  
Cho Cell ◽  
Cell Culture Media ◽  
Gene Expression Analyses ◽  
Tryptophan Oxidation ◽  
Cho Cell Culture ◽  
The Impact

scholarly journals Impact of Dissolved Oxygen during UV-Irradiation on the Chemical Composition and Function of CHO Cell Culture Media

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0150957 ◽  
Author(s):  
Sarah M. Meunier ◽  
Biljana Todorovic ◽  
Emma V. Dare ◽  
Afroza Begum ◽  
Simon Guillemette ◽  
...  
Keyword(s):  
Cell Culture ◽  
Chemical Composition ◽  
Dissolved Oxygen ◽  
Uv Irradiation ◽  
Culture Media ◽  
Cho Cell ◽  
Cell Culture Media ◽  
Cho Cell Culture ◽  
And Function

scholarly journals Benchmarking of commercially available CHO cell culture media for antibody production

2013 ◽  
Vol 7 (S6) ◽  
Author(s):  
David Reinhart ◽  
Christian Kaisermayer ◽  
Lukas Damjanovic ◽  
Renate Kunert
Keyword(s):  
Cell Culture ◽  
Antibody Production ◽  
Culture Media ◽  
Cho Cell ◽  
Cell Culture Media ◽  
Cho Cell Culture

scholarly journals The effect of hydrogen peroxide produced during ultraviolet disinfection of CHO cell culture media

2017 ◽  
Vol 61 ◽  
pp. 147-155 ◽  
Author(s):  
Emma V. Dare ◽  
Michelle Gabriel ◽  
Afroza Begum ◽  
Michael Sasges ◽  
Marc G. Aucoin
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Culture ◽  
Culture Media ◽  
Ultraviolet Disinfection ◽  
Cho Cell ◽  
Cell Culture Media ◽  
Cho Cell Culture

scholarly journals Screening Naturally Occurring Phenolic Antioxidants for Their Suitability as Additives to CHO Cell Culture Media Used to Produce Monoclonal Antibodies

Antioxidants ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 159 ◽  
Author(s):  
Luis Toronjo-Urquiza ◽  
David James ◽  
Tibor Nagy ◽  
Robert Falconer
Keyword(s):  
Cell Culture ◽  
Cell Density ◽  
Culture Media ◽  
Viable Cell ◽  
Viable Cell Density ◽  
Specific Productivity ◽  
Cho Cell ◽  
Cell Culture Media ◽  
Igg Synthesis ◽  
Cho Cell Culture

This study identified several antioxidants that could be used in Chinese hamster ovary (CHO)cell culture media and benefit monoclonal antibody production. The flavan-3-ols, catechin, epicatechin, epigallocatechin gallate and gallocatechin gallate all had no detrimental effect on cell viability at the concentrations tested, and they reduced the final viable cell count with a resulting rise in the cell specific productivity. The flavone, luteolin behave similarly to the flavan-3-ols. Resveratrol at 50 μM concentration resulted in the most pronounced reduction in viable cell density with minimal decrease in IgG synthesis and the largest increase in cell specific productivity. Low concentrations of α-tocopherol (35 μM) reduced viable cell density and raised cell specific productivity, but at higher concentration it had little additional effect. As high concentrations of α-tocopherol are not toxic to CHO cells, its addition as an anti-oxidant has great potential. Kaempferol up to 50 μM, curcumin up to 20 μM and piceid up to 100 μM showed little effect on growth or IgG synthesis and could be useful as antioxidants. Caffeic acid phenethyl ester was toxic to CHO cell and of no interest. Seven of the phenolic compounds tested are potential cell cycle inhibitors as well as having intrinsic antioxidant properties.

Peptide fractions of rapeseed hydrolysates as an alternative to animal proteins in CHO cell culture media

2006 ◽  
Vol 41 (11) ◽  
pp. 2297-2304 ◽  
Author(s):  
B. Farges-Haddani ◽  
B. Tessier ◽  
S. Chenu ◽  
I. Chevalot ◽  
C. Harscoat ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Cho Cell ◽  
Cell Culture Media ◽  
Cho Cell Culture ◽  
Peptide Fractions

scholarly journals Protein Content and Amino Acid Composition of Certain Fungi Evaluated for Microbial Protein Production

1975 ◽  
Vol 29 (2) ◽  
pp. 250-254
Author(s):  
C. Christias ◽  
C. Couvaraki ◽  
S. G. Georgopoulos ◽  
B. Macris ◽  
V. Vomvoyanni
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Protein Content ◽  
Acid Composition ◽  
Protein Production ◽  
Microbial Protein
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