scholarly journals U937 cell apoptosis induced by arsenite is prevented by low concentrations of mitochondrial ascorbic acid with hardly any effect mediated by the cytosolic fraction of the vitamin

BioFactors ◽  
2015 ◽  
Vol 41 (2) ◽  
pp. 101-110 ◽  
Author(s):  
Andrea Guidarelli ◽  
Mara Fiorani ◽  
Catia Azzolini ◽  
Liana Cerioni ◽  
Maddalena Scotti ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Cell Apoptosis ◽  
U937 Cell ◽  
Cytosolic Fraction ◽  
Low Concentrations

scholarly journals Hydrogen Peroxide Induces Hyphal Differentiation in Candida albicans

2008 ◽  
Vol 7 (11) ◽  
pp. 2008-2011 ◽  
Author(s):  
Olviyani Nasution ◽  
Kavitha Srinivasa ◽  
Minsun Kim ◽  
Yeo-Jung Kim ◽  
Wankee Kim ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Ascorbic Acid ◽  
Candida Albicans ◽  
Intracellular Concentration ◽  
Low Concentrations ◽  
Null Mutants

ABSTRACT In this study, we demonstrate that hyphal differentiation is induced by the subtoxic concentration of exogenous H2O2 in Candida albicans. This finding is confirmed by the changing intracellular concentration of H2O2. In order to induce the same level of differentiation, low concentrations of exogenous H2O2 are required for the null mutants of the thiol-specific antioxidant and catalase, while higher concentrations are needed for cells treated with ascorbic acid, an antioxidant chemical.

scholarly journals The Association of Ascorbic Acid, Deferoxamine and N-Acetylcysteine Improves Cardiac Fibroblast Viability and Cellular Function Associated with Tissue Repair Damaged by Simulated Ischemia/Reperfusion

Antioxidants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 614 ◽  
Author(s):  
Pablo Parra-Flores ◽  
Jaime A Riquelme ◽  
Paula Valenzuela-Bustamante ◽  
Sebastian Leiva-Navarrete ◽  
Raúl Vivar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Protein Kinase ◽  
Tissue Repair ◽  
Ischemia Reperfusion Injury ◽  
Cardiac Fibroblasts ◽  
Ischemia Reperfusion ◽  
Cardiac Fibroblast ◽  
Simulated Ischemia ◽  
Low Concentrations

Acute myocardial infarction is one of the leading causes of death worldwide and thus, an extensively studied disease. Nonetheless, the effects of ischemia/reperfusion injury elicited by oxidative stress on cardiac fibroblast function associated with tissue repair are not completely understood. Ascorbic acid, deferoxamine, and N-acetylcysteine (A/D/N) are antioxidants with known cardioprotective effects, but the potential beneficial effects of combining these antioxidants in the tissue repair properties of cardiac fibroblasts remain unknown. Thus, the aim of this study was to evaluate whether the pharmacological association of these antioxidants, at low concentrations, could confer protection to cardiac fibroblasts against simulated ischemia/reperfusion injury. To test this, neonatal rat cardiac fibroblasts were subjected to simulated ischemia/reperfusion in the presence or absence of A/D/N treatment added at the beginning of simulated reperfusion. Cell viability was assessed using trypan blue staining, and intracellular reactive oxygen species (ROS) production was assessed using a 2′,7′-dichlorofluorescin diacetate probe. Cell death was measured by flow cytometry using propidium iodide. Cell signaling mechanisms, differentiation into myofibroblasts and pro-collagen I production were determined by Western blot, whereas migration was evaluated using the wound healing assay. Our results show that A/D/N association using a low concentration of each antioxidant increased cardiac fibroblast viability, but that their separate administration did not provide protection. In addition, A/D/N association attenuated oxidative stress triggered by simulated ischemia/reperfusion, induced phosphorylation of pro-survival extracellular-signal-regulated kinases 1/2 (ERK1/2) and PKB (protein kinase B)/Akt, and decreased phosphorylation of the pro-apoptotic proteins p38- mitogen-activated protein kinase (p38-MAPK) and c-Jun-N-terminal kinase (JNK). Moreover, treatment with A/D/N also reduced reperfusion-induced apoptosis, evidenced by a decrease in the sub-G1 population, lower fragmentation of pro-caspases 9 and 3, as well as increased B-cell lymphoma-extra large protein (Bcl-xL)/Bcl-2-associated X protein (Bax) ratio. Furthermore, simulated ischemia/reperfusion abolished serum-induced migration, TGF-β1 (transforming growth factor beta 1)-mediated cardiac fibroblast-to-cardiac myofibroblast differentiation, and angiotensin II-induced pro-collagen I synthesis, but these effects were prevented by treatment with A/D/N. In conclusion, this is the first study where a pharmacological combination of A/D/N, at low concentrations, protected cardiac fibroblast viability and function after simulated ischemia/reperfusion, and thereby represents a novel therapeutic approach for cardioprotection.

scholarly journals Sodium-dependent transport of ascorbic acid in U937 cell mitochondria

IUBMB Life ◽  
2013 ◽  
Vol 65 (2) ◽  
pp. 149-153 ◽  
Author(s):  
Catia Azzolini ◽  
Mara Fiorani ◽  
Liana Cerioni ◽  
Andrea Guidarelli ◽  
Orazio Cantoni
Keyword(s):  
Ascorbic Acid ◽  
U937 Cell ◽  
Sodium Dependent ◽  
Dependent Transport

THE COLORIMETRIC DETERMINATION OF PLATINUM WITH 5-(p-DIMETHYLAMINOBENZYLIDENE)-RHODANINE

1963 ◽  
Vol 41 (3) ◽  
pp. 667-670 ◽  
Author(s):  
Frances E. Piercy ◽  
D. E. Ryan
Keyword(s):  
Ascorbic Acid ◽  
The Other ◽  
Colorimetric Determination ◽  
Platinum Metals ◽  
Low Concentrations ◽  
Fold Excess

5-(p-Dimethylaminobenzylidene)-rhodanine is used to determine 0.5 to 6 p.p.m. of platinum colorimetrically, after reduction of platinum IV with ascorbic acid. Platinum can be determined in the presence of a 10-fold excess of palladium; low concentrations of the other platinum metals, except rhodium, do not seriously interfere.

scholarly journals Flow-injection Analysis Technique Used to Electrochemically Measure Nitrite through a Gold Working Electrode Modified with 1-2 Diaminobenzene (DAB)

2010 ◽  
Vol 5 (2) ◽  
pp. 134-139
Author(s):  
F. L. Almeida ◽  
S. G. Dos Santos Filho ◽  
M. B. A. Fontes
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Human Health ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
Working Electrode ◽  
Low Concentrations ◽  
Analysis Technique ◽  
Silk Screen

In this paper, it is presented nitrite analysis, electrochemically measured, using a Flow-injection Analysis (FIA) system. The importance of determining nitrite from water, food and blood is based on its toxicity for human health, even at low concentrations (<4mM). The presented electrochemical planar sensor uses silk-screen technology and has a working electrode covered with 1-2 Diaminobenzene (DAB) that allowed good selectivity, reproductively, stability and sensitivity at 0.75 VAg/AgCl Nafion® 117, 0.5 VAg/AgCl Nafion® 117 and 0.3 VAg/AgCl Nafion® 117. It was noteworthy that nitrite response adds to the response of the studied interfering elements (paracetamol, ascorbic acid and uric acid) and it is predominant for concentrations lower than 175 μmol L-1. Finally, the nitrite sensitivity was 11.2 mA mol-1 L mm-2 using the working electrode recovered with DAB at 0.5 VAg/AgCl Nafion®117.

Effect of Copper on Ascorbic Acid Content, Redox Potential and Development of Oxidized Flavor in Milk of Cows and Buffaloes

1976 ◽  
Vol 39 (6) ◽  
pp. 397-400 ◽  
Author(s):  
V. UNNIKRISHNAN ◽  
D. SETHU RAO ◽  
M. BHIMASENA RAO
Keyword(s):  
Ascorbic Acid ◽  
Redox Potential ◽  
Acid Content ◽  
Ascorbic Acid Content ◽  
Buffalo Milk ◽  
Cow Milk ◽  
Marked Rise ◽  
Appreciable Change ◽  
Low Concentrations ◽  
Effect Of Copper

The effect of copper on ascorbic acid content, redox potential (Eh) of milk, and development of oxidized flavor is discussed. Oxidized flavor appeared in cow milk only after a marked rise in Eh due to the oxidation of ascorbic acid, whereas in buffalo milk oxidized flavor developed without any appreciable change in Eh. In buffalo milk, copper at low concentrations failed to cause sufficient oxidation of ascorbic acid to effect an increase in Eh. The Eh of cow milk began to rise appreciably when more than half of the ascorbic acid normally present in milk was oxidized. The pro-oxidant action of ascorbic acid in cow milk at concentrations normal to milk is not merely due to conversion of copper to cuprous form; it is suggested that the activation energy involved in this conversion is an important factor in the lipid oxidation which follows. Ascorbic acid did not promote copper-catalyzed oxidation of buffalo milk as it is not involved in the reversible oxidation.

Ascorbic Acid Is a Potent Inhibitor of Various Forms of T Cell Apoptosis

1999 ◽  
Vol 194 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Jeff D. Campbell ◽  
Michael Cole ◽  
Bongkot Bunditrutavorn ◽  
Anthony T. Vella
Keyword(s):  
Ascorbic Acid ◽  
T Cell ◽  
Cell Apoptosis ◽  
Potent Inhibitor ◽  
T Cell Apoptosis

808 nm-light-excited upconversion nanoprobe based on LRET for the ratiometric detection of nitric oxide in living cancer cells

Nanoscale ◽  
2018 ◽  
Vol 10 (22) ◽  
pp. 10641-10649 ◽  
Author(s):  
Han Wang ◽  
Yi Liu ◽  
Zhaohui Wang ◽  
Man Yang ◽  
Yueqing Gu
Keyword(s):  
Nitric Oxide ◽  
Tumor Cell ◽  
Cancer Cells ◽  
Cell Apoptosis ◽  
Tumor Cell Apoptosis ◽  
Low Concentrations ◽  
Ratiometric Detection ◽  
High Concentrations ◽  
Dual Functions

NO (nitric oxide) has dual functions in cancer, promoting carcinogenesis in low concentrations and inducing tumor cell apoptosis at high concentrations.

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