Protein detection based on rolling circle amplification sensors

Luminescence ◽  
2021 ◽  
Author(s):  
Haixia Shi ◽  
Jingjie Cui ◽  
Husseini Sulemana ◽  
Wunian Wang ◽  
Li Gao
Keyword(s):  
Rolling Circle Amplification ◽  
Protein Detection ◽  
Rolling Circle

An On-Nanoparticle Rolling-Circle Amplification Platform for Ultrasensitive Protein Detection in Biological Fluids

Small ◽  
2010 ◽  
Vol 6 (22) ◽  
pp. 2520-2525 ◽  
Author(s):  
Juan Yan ◽  
Shiping Song ◽  
Bing Li ◽  
Qingzhi Zhang ◽  
Qing Huang ◽  
...  
Keyword(s):  
Biological Fluids ◽  
Rolling Circle Amplification ◽  
Protein Detection ◽  
Rolling Circle

Aptamer-based rolling circle amplification coupled with graphene oxide-based fluorescence resonance energy transfer for sensitive detection of cardiac troponin I

2018 ◽  
Vol 10 (15) ◽  
pp. 1767-1773 ◽  
Author(s):  
Yu Li ◽  
Weiqiang Dai ◽  
Xuefei Lv ◽  
Yulin Deng
Keyword(s):  
Graphene Oxide ◽  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Troponin I ◽  
Rolling Circle Amplification ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Protein Detection ◽  
Rolling Circle ◽  
Fluorescence Resonance

Combining the high affinity and specificity of an aptamer for a protein, a highly sensitive strategy for protein detection was developed based on rolling circle amplification (RCA) and graphene oxide based fluorescence resonance energy transfer (FRET).

scholarly journals Analytically Sensitive Protein Detection in Microtiter Plates by Proximity Ligation with Rolling Circle Amplification

2017 ◽  
Vol 63 (9) ◽  
pp. 1497-1505 ◽  
Author(s):  
Tonge Ebai ◽  
Felipe Marques Souza de Oliveira ◽  
Liza Löf ◽  
Lotta Wik ◽  
Caroline Schweiger ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Target Recognition ◽  
Rolling Circle Amplification ◽  
Sandwich Elisa ◽  
Protein Detection ◽  
Proximity Ligation Assay ◽  
Clinical Samples ◽  
Rolling Circle ◽  
Proximity Ligation ◽  
Lower Protein

Abstract BACKGROUND Detecting proteins at low concentrations in plasma is crucial for early diagnosis. Current techniques in clinical routine, such as sandwich ELISA, provide sensitive protein detection because of a dependence on target recognition by pairs of antibodies, but detection of still lower protein concentrations is often called for. Proximity ligation assay with rolling circle amplification (PLARCA) is a modified proximity ligation assay (PLA) for analytically specific and sensitive protein detection via binding of target proteins by 3 antibodies, and signal amplification via rolling circle amplification (RCA) in microtiter wells, easily adapted to instrumentation in use in hospitals. METHODS Proteins captured by immobilized antibodies were detected using a pair of oligonucleotide-conjugated antibodies. Upon target recognition these PLA probes guided oligonucleotide ligation, followed by amplification via RCA of circular DNA strands that formed in the reaction. The RCA products were detected by horseradish peroxidase-labeled oligonucleotides to generate colorimetric reaction products with readout in an absorbance microplate reader. RESULTS We compared detection of interleukin (IL)-4, IL-6, IL-8, p53, and growth differentiation factor 15 (GDF-15) by PLARCA and conventional sandwich ELISA or immuno-RCA. PLARCA detected lower concentrations of proteins and exhibited a broader dynamic range compared to ELISA and iRCA using the same antibodies. IL-4 and IL-6 were detected in clinical samples at femtomolar concentrations, considerably lower than for ELISA. CONCLUSIONS PLARCA offers detection of lower protein levels and increased dynamic ranges compared to ELISA. The PLARCA procedure may be adapted to routine instrumentation available in hospitals and research laboratories.

Real-Time Rolling Circle Amplification for Protein Detection

2007 ◽  
Vol 79 (9) ◽  
pp. 3320-3329 ◽  
Author(s):  
Litao Yang ◽  
Christine W. Fung ◽  
Eun Jeong Cho ◽  
Andrew D. Ellington
Keyword(s):  
Real Time ◽  
Rolling Circle Amplification ◽  
Protein Detection ◽  
Rolling Circle
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