A protein detection technique by using surface plasmon resonance (SPR) with rolling circle amplification (RCA) and nanogold-modified tags

2007 ◽  
Vol 22 (6) ◽  
pp. 980-985 ◽  
Author(s):  
Yi-You Huang ◽  
Hsin-Yun Hsu ◽  
Chi-Jer Charles Huang
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Rolling Circle Amplification ◽  
Protein Detection ◽  
Detection Technique ◽  
Rolling Circle

Ultrasensitive detection of thrombin using surface plasmon resonance and quartz crystal microbalance sensors by aptamer-based rolling circle amplification and nanoparticle signal enhancement

2014 ◽  
Vol 50 (12) ◽  
pp. 1481-1484 ◽  
Author(s):  
Peng He ◽  
Lijun Liu ◽  
Wenping Qiao ◽  
Shusheng Zhang
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Quartz Crystal Microbalance ◽  
Plasmon Resonance ◽  
Quartz Crystal ◽  
Rolling Circle Amplification ◽  
Ultrasensitive Detection ◽  
Sandwich Assay ◽  
Rolling Circle ◽  
First Time

The SPR and QCM aptasensors combined with RCA, AuNP enhancement, and sandwich assay format have been applied to detect the human α-thrombin for the first time.

scholarly journals Surface Plasmon Resonance for C-Reactive Protein Detection in Human Plasma

2014 ◽  
Vol 05 (03) ◽  
pp. 153-158 ◽  
Author(s):  
Hanen Chammem ◽  
Imen Hafaid ◽  
Olivier Meilhac ◽  
Farid Menaa ◽  
Laurence Mora ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Human Plasma ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Protein Detection ◽  
C Reactive Protein ◽  
Reactive Protein

Localized Surface Plasmon Resonance (LSPR) Biosensor for the Protein Detection

Plasmonics ◽  
2012 ◽  
Vol 8 (2) ◽  
pp. 699-704 ◽  
Author(s):  
Maximilien Cottat ◽  
Néné Thioune ◽  
Ana-Maria Gabudean ◽  
Nathalie Lidgi-Guigui ◽  
Monica Focsan ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Protein Detection ◽  
Localized Surface Plasmon

scholarly journals Low-Cost Localized Surface Plasmon Resonance Biosensing Platform with a Response Enhancement for Protein Detection

Nanomaterials ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 1019 ◽  
Author(s):  
Yun Liu ◽  
Ning Zhang ◽  
Ping Li ◽  
Li Yu ◽  
Shimeng Chen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Low Cost ◽  
Microfluidic Channel ◽  
Protein Detection ◽  
Localized Surface Plasmon

There are many potential applications for biosensors that can provide real-time analysis, such as environmental monitoring and disease prevention. In this study, we investigated a simple strategy for real-time protein detection, which had the advantages of affordability, fast response, portability, and ease of use. A robust quantification of protein interaction was achieved by combining capillary localized surface plasmon resonance (LSPR) sensors and complementary metal–oxide–semiconductor (CMOS) image sensors. Gold nanoparticles were modified on the inner wall of the capillary, which was used as a microfluidic channel and sensing surface. We functionalized one of the LSPR sensors using ligand bound to gold nanoparticle. Our proposed biosensing platform could be easily multiplexed to achieve high throughput screening of biomolecular interactions, and it has the potential for use in disposable sensors. Moreover, the sensing signal was enhanced by the extinction effect of gold nanoparticles. The experimental results showed that our device could achieve qualitative identification and quantitative measurement of transferrin and immunoglobulin G (IgG). As a field-portable and low-cost optical platform, the proposed LSPR biosensing device is broadly applicable to various protein binding tests via a similar self-assembly of organic ultrathin films.

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