A caged 2‐hydroxyethyl luciferin for bioluminescence imaging of nitroxyl in living cells

Luminescence ◽  
2020 ◽  
Vol 35 (8) ◽  
pp. 1384-1390
Author(s):  
Yong Ju ◽  
Anni Wang ◽  
Xuewei Li ◽  
Xu Xu ◽  
Jianzhong Lu
Keyword(s):  
Bioluminescence Imaging ◽  
Living Cells

scholarly journals Autonomous bioluminescence imaging of single mammalian cells with the bacterial bioluminescence system

2019 ◽  
Vol 116 (52) ◽  
pp. 26491-26496 ◽  
Author(s):  
Carola Gregor ◽  
Jasmin K. Pape ◽  
Klaus C. Gwosch ◽  
Tanja Gilat ◽  
Steffen J. Sahl ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Bioluminescence Imaging ◽  
Single Cells ◽  
Cell Types ◽  
Firefly Luciferase ◽  
Living Cells ◽  
Spatiotemporal Resolution ◽  
Bacterial Bioluminescence ◽  
Mammalian Cell Lines ◽  
Bioluminescence Signal

Bioluminescence-based imaging of living cells has become an important tool in biological and medical research. However, many bioluminescence imaging applications are limited by the requirement of an externally provided luciferin substrate and the low bioluminescence signal which restricts the sensitivity and spatiotemporal resolution. The bacterial bioluminescence system is fully genetically encodable and hence produces autonomous bioluminescence without an external luciferin, but its brightness in cell types other than bacteria has, so far, not been sufficient for imaging single cells. We coexpressed codon-optimized forms of the bacterialluxCDABEandfrpgenes from multiple plasmids in different mammalian cell lines. Our approach produces high luminescence levels that are comparable to firefly luciferase, thus enabling autonomous bioluminescence microscopy of mammalian cells.

Bioluminescence imaging of carbon monoxide in living cells based on a selective deiodination reaction

The Analyst ◽  
2020 ◽  
Vol 145 (2) ◽  
pp. 550-556 ◽  
Author(s):  
Anni Wang ◽  
Xuewei Li ◽  
Yong Ju ◽  
Dongying Chen ◽  
Jianzhong Lu
Keyword(s):  
Carbon Monoxide ◽  
Cancer Cells ◽  
Iodine Atom ◽  
Bioluminescence Imaging ◽  
Living Cells ◽  
Turn On ◽  
Co Detection

Modification of a heavy iodine atom for d-Luciferin was explored as a “turn-on” transduction scheme for CO detection. This new probe could image exogenous and endogenous CO in the luciferase-transfected cancer cells.

Video rate bioluminescence imaging of secretory proteins in living cells: Localization, secretory frequency, and quantification

2011 ◽  
Vol 415 (2) ◽  
pp. 182-189 ◽  
Author(s):  
Takahiro Suzuki ◽  
Chihiro Kondo ◽  
Takao Kanamori ◽  
Satoshi Inouye
Keyword(s):  
Bioluminescence Imaging ◽  
Living Cells ◽  
Secretory Proteins

scholarly journals Prolonged bioluminescence imaging in living cells and mice using novel pro-substrates forRenillaluciferase

2017 ◽  
Vol 15 (48) ◽  
pp. 10238-10244 ◽  
Author(s):  
Mingliang Yuan ◽  
Xiaojie Ma ◽  
Tianyu Jiang ◽  
Yuqi Gao ◽  
Yuanyuan Cui ◽  
...  
Keyword(s):  
Bioluminescence Imaging ◽  
Living Cells

The prodrug or caged-luciferin strategy affords an excellent platform for persistent bioluminescence imaging.

Bioluminescence Imaging of Carbon Monoxide in Living Cells and Nude Mice Based on Pd0-Mediated Tsuji–Trost Reaction

2018 ◽  
Vol 90 (9) ◽  
pp. 5951-5958 ◽  
Author(s):  
Xiaodong Tian ◽  
Xinda Liu ◽  
Anni Wang ◽  
Choiwan Lau ◽  
Jianzhong Lu
Keyword(s):  
Carbon Monoxide ◽  
Nude Mice ◽  
Bioluminescence Imaging ◽  
Living Cells

Fluorescence ratio imaging of dynamic intracellular ionic signals

1988 ◽  
Vol 46 ◽  
pp. 42-43
Author(s):  
R. Y. Tsien ◽  
A. Minta ◽  
M. Poenie ◽  
J.P.Y. Kao ◽  
A. Harootunian
Keyword(s):  
Binding Sites ◽  
Living Cells ◽  
Molecular Engineering ◽  
Ph Indicators ◽  
Highly Sensitive ◽  
Fluorescence Ratio Imaging ◽  
Ratio Imaging ◽  
Technical Advances ◽  
Indicator Dyes ◽  
Fluorescein Dyes

Recent technical advances now enable the continuous imaging of important ionic signals inside individual living cells with micron spatial resolution and subsecond time resolution. This methodology relies on the molecular engineering of indicator dyes whose fluorescence is strong and highly sensitive to ions such as Ca2+, H+, or Na+, or Mg2+. The Ca2+ indicators, exemplified by fura-2 and indo-1, derive their high affinity (Kd near 200 nM) and selectivity for Ca2+ to a versatile tetracarboxylate binding site3 modeled on and isosteric with the well known chelator EGTA. The most commonly used pH indicators are fluorescein dyes (such as BCECF) modified to adjust their pKa's and improve their retention inside cells. Na+ indicators are crown ethers with cavity sizes chosen to select Na+ over K+: Mg2+ indicators use tricarboxylate binding sites truncated from those of the Ca2+ chelators, resulting in a more compact arrangement of carboxylates to suit the smaller ion.

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

Multimode light microscopy and fluorescence-based reagents as tools for the study of chemical and molecular dynamics of living cells and tissues

1992 ◽  
Vol 50 (1) ◽  
pp. 418-419
Author(s):  
D. L. Taylor
Keyword(s):  
Living Cells ◽  
Cellular Level ◽  
Molecular Techniques ◽  
Cellular Functions ◽  
Macromolecular Assemblies ◽  
Cell Functions ◽  
Molecular Events ◽  
Yield Information ◽  
Full Knowledge ◽  
Structural Methods

Cells function through the complex temporal and spatial interplay of ions, metabolites, macromolecules and macromolecular assemblies. Biochemical approaches allow the investigator to define the components and the solution chemical reactions that might be involved in cellular functions. Static structural methods can yield information concerning the 2- and 3-D organization of known and unknown cellular constituents. Genetic and molecular techniques are powerful approaches that can alter specific functions through the manipulation of gene products and thus identify necessary components and sequences of molecular events. However, full knowledge of the mechanism of particular cell functions will require direct measurement of the interplay of cellular constituents. Therefore, there has been a need to develop methods that can yield chemical and molecular information in time and space in living cells, while allowing the integration of information from biochemical, molecular and genetic approaches at the cellular level.

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