Bioluminescence imaging of carbon monoxide in living cells based on a selective deiodination reaction

The Analyst ◽  
2020 ◽  
Vol 145 (2) ◽  
pp. 550-556 ◽  
Author(s):  
Anni Wang ◽  
Xuewei Li ◽  
Yong Ju ◽  
Dongying Chen ◽  
Jianzhong Lu
Keyword(s):  
Carbon Monoxide ◽  
Cancer Cells ◽  
Iodine Atom ◽  
Bioluminescence Imaging ◽  
Living Cells ◽  
Turn On ◽  
Co Detection

Modification of a heavy iodine atom for d-Luciferin was explored as a “turn-on” transduction scheme for CO detection. This new probe could image exogenous and endogenous CO in the luciferase-transfected cancer cells.

A metal-free coumarin-based fluorescent probe for the turn-on monitoring of carbon monoxide in an aqueous solution and living cells

The Analyst ◽  
2021 ◽  
Author(s):  
Shiqing Zhang ◽  
Xinyue Mu ◽  
Jinbiao Zhu ◽  
Liqiang Yan
Keyword(s):  
Aqueous Solution ◽  
Carbon Monoxide ◽  
Fluorescent Probe ◽  
Simple Structure ◽  
Living Cells ◽  
Detection Performance ◽  
Metal Free ◽  
Turn On ◽  
Co Detection

A fluorescent probe for CO detection was prepared, showing a simple structure, being metal-free, and having an excellent detection performance.

A naphthalimide-based fluorescence ‘‘turn-on’’ chemosensor for highly selective detection of carbon monoxide: imaging applications in living cells

2018 ◽  
Vol 42 (16) ◽  
pp. 13497-13502 ◽  
Author(s):  
Biswajit Das ◽  
Somenath Lohar ◽  
Ayan Patra ◽  
Ejaj Ahmmed ◽  
Sushil Kumar Mandal ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Living Cells ◽  
Selective Detection ◽  
Hepes Buffer ◽  
Turn On ◽  
Fluorescence Turn On

A naphthalimide-based fluorescence chemosensor, COFP, was designed and synthesized for the detection of carbon monoxide (CO) in HEPES buffer (pH 7.4, 37 °C).

A New Lysosome-Targetable Turn-On Fluorogenic Probe for Carbon Monoxide Imaging in Living Cells

2018 ◽  
Vol 90 (4) ◽  
pp. 2933-2938 ◽  
Author(s):  
Koushik Dhara ◽  
Somenath Lohar ◽  
Ayan Patra ◽  
Priya Roy ◽  
Swadhin Kumar Saha ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Living Cells ◽  
Turn On ◽  
Fluorogenic Probe

scholarly journals A colorimetric and fluorescent turn-on probe for carbon monoxide and imaging in living cells

2016 ◽  
Vol 57 (26) ◽  
pp. 2927-2930 ◽  
Author(s):  
Zhongyong Xu ◽  
Jinwu Yan ◽  
Jing Li ◽  
Peifen Yao ◽  
Jiaheng Tan ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Living Cells ◽  
Turn On

Bioluminescence Imaging of Carbon Monoxide in Living Cells and Nude Mice Based on Pd0-Mediated Tsuji–Trost Reaction

2018 ◽  
Vol 90 (9) ◽  
pp. 5951-5958 ◽  
Author(s):  
Xiaodong Tian ◽  
Xinda Liu ◽  
Anni Wang ◽  
Choiwan Lau ◽  
Jianzhong Lu
Keyword(s):  
Carbon Monoxide ◽  
Nude Mice ◽  
Bioluminescence Imaging ◽  
Living Cells

scholarly journals A Novel Nanobody Precisely Visualizes Phosphorylated Histone H2AX in Living Cancer Cells under Drug-Induced Replication Stress

Cancers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 3317
Author(s):  
Eric Moeglin ◽  
Dominique Desplancq ◽  
Audrey Stoessel ◽  
Christian Massute ◽  
Jeremy Ranniger ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Mammalian Cells ◽  
Chromatin Modification ◽  
Replication Stress ◽  
Living Cells ◽  
Single Step ◽  
Dna Double Strand Breaks ◽  
Drug Induced ◽  
Histone H2ax ◽  
C Terminus

Histone H2AX phosphorylated at serine 139 (γ-H2AX) is a hallmark of DNA damage, signaling the presence of DNA double-strand breaks and global replication stress in mammalian cells. While γ-H2AX can be visualized with antibodies in fixed cells, its detection in living cells was so far not possible. Here, we used immune libraries and phage display to isolate nanobodies that specifically bind to γ-H2AX. We solved the crystal structure of the most soluble nanobody in complex with the phosphopeptide corresponding to the C-terminus of γ-H2AX and show the atomic constituents behind its specificity. We engineered a bivalent version of this nanobody and show that bivalency is essential to quantitatively visualize γ-H2AX in fixed drug-treated cells. After labelling with a chemical fluorophore, we were able to detect γ-H2AX in a single-step assay with the same sensitivity as with validated antibodies. Moreover, we produced fluorescent nanobody-dTomato fusion proteins and applied a transduction strategy to visualize with precision γ-H2AX foci present in intact living cells following drug treatment. Together, this novel tool allows performing fast screenings of genotoxic drugs and enables to study the dynamics of this particular chromatin modification in individual cancer cells under a variety of conditions.

“Turn‐on” Fluorescence Chemosensor Based Probing of Cu 2+ with Excellent Sensitivity: Experimental Study, DFT Calculations and Application in Living Cells and Natural Waters

2021 ◽  
Vol 6 (24) ◽  
pp. 6286-6294
Author(s):  
Tahir Savran ◽  
Sukriye Nihan Karuk Elmas ◽  
Gonul Akin Geyik ◽  
Aykut Bostanci ◽  
Duygu Aydin ◽  
...  
Keyword(s):  
Experimental Study ◽  
Dft Calculations ◽  
Natural Waters ◽  
Living Cells ◽  
Turn On

Photoactive conjugated polymer/graphdiyne nanocatalyst for CO2 reduce into CO in living cells for hypoxia tumor treatment

2021 ◽  
Author(s):  
Endong Zhang ◽  
Zicheng Zuo ◽  
Wen Yu ◽  
Hao Zhao ◽  
Shengpeng Xia ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Photodynamic Therapy ◽  
Tumor Microenvironment ◽  
Conjugated Polymer ◽  
Tumor Therapy ◽  
Living Cells ◽  
Tumor Treatment ◽  
Treatment Efficiency ◽  
Therapy Strategy ◽  
Co Gas

Carbon monoxide (CO) gas therapy has grown to be an emerging tumor therapy strategy to avoid low treatment efficiency of photodynamic therapy (PDT) caused by the hypoxia tumor microenvironment. However,...

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