Highly sensitive spectrofluorimetric method for rapid determination of orciprenaline in biological fluids and pharmaceuticals

Luminescence ◽  
2018 ◽  
Author(s):  
F.A. Ibrahim ◽  
M.E. Fathy ◽  
H.M. Elmansi
Keyword(s):  
Rapid Determination ◽  
Biological Fluids ◽  
Spectrofluorimetric Method ◽  
Highly Sensitive

A Fluorometric Ferric Chloride Method for Determining Cholesterol in Cerebrospinal Fluid and Serum

1970 ◽  
Vol 16 (6) ◽  
pp. 472-476 ◽  
Author(s):  
Elizabeth B Solow ◽  
L W Freeman
Keyword(s):  
Cerebrospinal Fluid ◽  
Ferric Chloride ◽  
Rapid Determination ◽  
Biological Fluids ◽  
The Past ◽  
Highly Sensitive ◽  
Chloride Method ◽  
Cholesterol Measurement

Abstract Sensitive or simple methods for the rapid determination of cholesterol in biological fluids have been developed during the past 10 years. Sensitivity has been increased by fluorimetry of the Lieberman—Burchard reaction for cholesterol. Measurement of the reaction of cholesterol with ferric chloride is simpler. Still, there are great differences between the results when different methods are used to measure the microquantities of cholesterol present in small volumes of serum or cerebrospinal fluid. In the proposed method, the simpler ferric chloride technique has been made highly sensitive by use of fluorometry. As little as 100 µl of cerebrospinal fluid, containing less than 1 µg of cholesterol, may be used, and the reaction is stable for as long as 1 h. Interference was negligible from pigments (such as bilirubin and hemoglobin), certain drugs, and ionic substances that might be expected to affect fluorescence.

Green highly sensitive spectrofluorimetric method for rapid determination of tafluprost in its pure form and ophthalmic formulation

Luminescence ◽  
2020 ◽  
Vol 35 (8) ◽  
pp. 1264-1268
Author(s):  
Walaa Nabil Abd‐AlGhafar ◽  
Samar Saad ◽  
Zainab Sheribah ◽  
Fatma Aly
Keyword(s):  
Rapid Determination ◽  
Pure Form ◽  
Spectrofluorimetric Method ◽  
Highly Sensitive ◽  
Ophthalmic Formulation

Solid-phase radioimmunoassay for morphine, with use of an affinity-purified morphine antibody.

1978 ◽  
Vol 24 (2) ◽  
pp. 339-342 ◽  
Author(s):  
M Steiner ◽  
J L Spratt
Keyword(s):  
Affinity Chromatography ◽  
Correlation Coefficient ◽  
Solid Phase ◽  
Rapid Determination ◽  
Biological Fluids ◽  
Inhibitory Effect ◽  
Major Metabolite ◽  
Solid Phase Radioimmunoassay ◽  
Related Compounds

Abstract Morphine antibody purified by affinity chromatography was used to develop a solid-phase radioimmunoassay for morphine in polystyrene tubes. The tubes are coated with an appropriate concentration of the purified antibody, rinsed three times with buffered saline, and stored at -15 degrees C. Using tritiated dihydromorphine, we determined competitive morphine binding by difference when the radioactivity in the assay supernates was measured after incubation (1 h, 37 degrees C). Five standard curves, with use of serum equivalents of morphine ranging from 0 to 6 mug/liter, were linear and had a mean correlation coefficient of 0.98. Uncer conditions of the assay, levorphanol was comparable to morphine in its inhibitory effect on binding of labeled dihydromorphine, whereas dextrorphan was essentially inactive. Morphine-3-glucuronide, a major metabolite, is 55-fold less inhibitory in terms of its capacity to displace the radiolabel. We believe that the sensitivity of the technique, coupled with the simplicity of nonseparatory sampling, renders the system suitable for rapid determination of morphine and related compounds in biological fluids.

A Highly Sensitive and Selective Spectrofluorimetric Method for the Determination of Nitrite in Food Products

2015 ◽  
Vol 9 (5) ◽  
pp. 1293-1300 ◽  
Author(s):  
Qiuhua Wang ◽  
Haiwei Huang ◽  
Baoming Ning ◽  
Minfeng Li ◽  
Lan He
Keyword(s):  
Food Products ◽  
Spectrofluorimetric Method ◽  
Highly Sensitive

Determination of Total Urinary Protein, Combining Lowry Sensitivity and Biuret Specificity

1973 ◽  
Vol 19 (10) ◽  
pp. 1170-1178 ◽  
Author(s):  
Karl Doetsch ◽  
Richard H Gadsden
Keyword(s):  
Ion Exchange ◽  
Copper Complex ◽  
Biological Fluids ◽  
Gel Filtration ◽  
Urinary Protein ◽  
Peptide Bonds ◽  
Highly Sensitive ◽  
Filtration Step ◽  
Sensitive Method

Abstract A highly sensitive method with specificity for the peptide chain backbone was developed for determination of total urinary protein. Interfering substances are removed by gel filtration and cupric ions are stoichiometrically bound to the peptide bonds of protein by the biuret reaction. Ion-exchange characteristics of the gel are neutralized, allowing protein—copper complex to be separated from excess cupric ions by a second gel filtration step. Copper bound to peptide bonds is colorimetrically determined by use of sodium diethyldithiocarbamate. Nonprotein substances do not interfere unless they simultaneously bind to protein and chelate copper. As little as 1 mg of total protein per deciliter can be determined in heterogeneous biological fluids such as urine.

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