scholarly journals Rapid determination of salicylate in biological fluids

1954 ◽  
Vol 57 (2) ◽  
pp. 301-303 ◽  
Author(s):  
P. Trinder
Keyword(s):  
Rapid Determination ◽  
Biological Fluids

Solid-phase radioimmunoassay for morphine, with use of an affinity-purified morphine antibody.

1978 ◽  
Vol 24 (2) ◽  
pp. 339-342 ◽  
Author(s):  
M Steiner ◽  
J L Spratt
Keyword(s):  
Affinity Chromatography ◽  
Correlation Coefficient ◽  
Solid Phase ◽  
Rapid Determination ◽  
Biological Fluids ◽  
Inhibitory Effect ◽  
Major Metabolite ◽  
Solid Phase Radioimmunoassay ◽  
Related Compounds

Abstract Morphine antibody purified by affinity chromatography was used to develop a solid-phase radioimmunoassay for morphine in polystyrene tubes. The tubes are coated with an appropriate concentration of the purified antibody, rinsed three times with buffered saline, and stored at -15 degrees C. Using tritiated dihydromorphine, we determined competitive morphine binding by difference when the radioactivity in the assay supernates was measured after incubation (1 h, 37 degrees C). Five standard curves, with use of serum equivalents of morphine ranging from 0 to 6 mug/liter, were linear and had a mean correlation coefficient of 0.98. Uncer conditions of the assay, levorphanol was comparable to morphine in its inhibitory effect on binding of labeled dihydromorphine, whereas dextrorphan was essentially inactive. Morphine-3-glucuronide, a major metabolite, is 55-fold less inhibitory in terms of its capacity to displace the radiolabel. We believe that the sensitivity of the technique, coupled with the simplicity of nonseparatory sampling, renders the system suitable for rapid determination of morphine and related compounds in biological fluids.

Rapid determination of deuterium oxide in biological fluids

1960 ◽  
Vol 15 (2) ◽  
pp. 309-310 ◽  
Author(s):  
M. D. Turner ◽  
William A. Neely ◽  
James D. Hardy
Keyword(s):  
Rapid Determination ◽  
Biological Fluids ◽  
Deuterium Oxide

A Fluorometric Ferric Chloride Method for Determining Cholesterol in Cerebrospinal Fluid and Serum

1970 ◽  
Vol 16 (6) ◽  
pp. 472-476 ◽  
Author(s):  
Elizabeth B Solow ◽  
L W Freeman
Keyword(s):  
Cerebrospinal Fluid ◽  
Ferric Chloride ◽  
Rapid Determination ◽  
Biological Fluids ◽  
The Past ◽  
Highly Sensitive ◽  
Chloride Method ◽  
Cholesterol Measurement

Abstract Sensitive or simple methods for the rapid determination of cholesterol in biological fluids have been developed during the past 10 years. Sensitivity has been increased by fluorimetry of the Lieberman—Burchard reaction for cholesterol. Measurement of the reaction of cholesterol with ferric chloride is simpler. Still, there are great differences between the results when different methods are used to measure the microquantities of cholesterol present in small volumes of serum or cerebrospinal fluid. In the proposed method, the simpler ferric chloride technique has been made highly sensitive by use of fluorometry. As little as 100 µl of cerebrospinal fluid, containing less than 1 µg of cholesterol, may be used, and the reaction is stable for as long as 1 h. Interference was negligible from pigments (such as bilirubin and hemoglobin), certain drugs, and ionic substances that might be expected to affect fluorescence.

Rapid determination of levofloxacin in pharmaceuticals and biological fluids using a new chemiluminescence system

2011 ◽  
Vol 66 (1) ◽  
pp. 102-107 ◽  
Author(s):  
Xiaodong Shao ◽  
Ying Li ◽  
Yangqin Liu ◽  
Zhenghua Song
Keyword(s):  
Rapid Determination ◽  
Biological Fluids

Calibration of pre-equilibrium HF-LPME and its application to the rapid determination of free analytes in biological fluids

2015 ◽  
Vol 980 ◽  
pp. 28-33 ◽  
Author(s):  
Baiqiu Xu ◽  
Min Chen ◽  
Jinxing Hou ◽  
Xi Chen ◽  
Xu Zhang ◽  
...  
Keyword(s):  
Rapid Determination ◽  
Biological Fluids
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