Synthesis and application of thiol-functionalized magnetic nanoparticles for studying interactions of epirubicin hydrochloride with bovine serum albumin by fluorescence spectrometry

Luminescence ◽  
2016 ◽  
Vol 32 (2) ◽  
pp. 142-148 ◽  
Author(s):  
Huixia Xiong ◽  
Quanping Diao ◽  
Rui Jin ◽  
Daqian Song ◽  
Xinghua Wang ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Fluorescence Spectrometry ◽  
Functionalized Magnetic Nanoparticles

IMMOBILIZATION OF BSA ON DENDRIMER FUNCTIONALIZED MAGNETIC NANOPARTICLES

2011 ◽  
Vol 10 (04n05) ◽  
pp. 919-923 ◽  
Author(s):  
S. CHANDRA ◽  
N. NITHYAMATHI ◽  
P. SELVAMANI ◽  
D. BAHADUR
Keyword(s):  
Particle Size ◽  
Iron Oxide ◽  
Magnetic Nanoparticles ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Ftir Spectroscopy ◽  
Magnetite Nanoparticles ◽  
Bovine Serum ◽  
Oxide Nanoparticles ◽  
Functionalized Magnetic Nanoparticles

Iron oxide nanoparticles were prepared and functionalized by succinamide based dendrimer. The resultant particles were characterized by XRD, VSM, and FTIR spectroscopy. The results indicate that the dendrimers has effectively functionalized the magnetite nanoparticles which remain dispersive and exhibited super-paramagnetism with a magnetization value of 33.2 emu/g in a field of 2T. Mean particle size as calculated from the AFM was found to be ~ 23 nm. Bovine serum albumin was immobilized on the magnetic nanoparticles as was confirmed by the FTIR results.

Solventless mechanochemical preparation of novel magnetic bioconjugates

2017 ◽  
Vol 53 (54) ◽  
pp. 7635-7637 ◽  
Author(s):  
Daily Rodriguez-Padrón ◽  
Alain R. Puente-Santiago ◽  
Alina M. Balu ◽  
Antonio A. Romero ◽  
Rafael Luque
Keyword(s):  
Iron Oxide ◽  
Magnetic Nanoparticles ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Mechanochemical Synthesis ◽  
Bovine Serum ◽  
Iron Oxide Magnetic Nanoparticles

A solventless mechanochemical approach was employed to obtain a bioconjugate (BSA–DA–Fe2O3) based on bovine serum albumin (BSA) and dopamine (DA) coated iron oxide magnetic nanoparticles. BSA molecules retained their native-like structure after the mechanochemical synthesis.

Degradation of BSA Coating on Magnetite Nanoparticles

2014 ◽  
Vol 782 ◽  
pp. 607-610 ◽  
Author(s):  
Kornel Csach ◽  
Alena Juríková ◽  
Jozef Miškuf ◽  
Martina Koneracká ◽  
Vlasta Závišová ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Thermogravimetric Analysis ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Magnetite Nanoparticles ◽  
Bovine Serum ◽  
Magnetic Particle ◽  
Particle Surface ◽  
Protein Bovine Serum Albumin

Magnetic nanoparticles used in biomedicine have to be biocompatible what can be achieved by the modification of the magnetic particle surface with an appropriate biocompatible substance. In the work protein bovine serum albumin (BSA) was chosen to modify the surface of magnetite nanoparticles. BSA coated magnetite nanoparticles (MFBSA) with different feed weight ratios of BSA to the magnetite Fe3O4were prepared and thermally characterized using thermogravimetric analysis.

scholarly journals Superior Performance of Magnetic Nanoparticles for Entrapment and Fixation of Bovine Serum Albumin In-Vitro

2020 ◽  
Vol 30 (4) ◽  
Author(s):  
Mansour Binandeh ◽  
Farrokh Karimi ◽  
Sadegh Rostamnia
Keyword(s):  
Magnetic Nanoparticles ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Superior Performance ◽  
Precipitation Method ◽  
Experimental Conditions ◽  
Ft Ir ◽  
Co Precipitation

BACKGROUND: In recent years, extensive studies have been performed on magnetite nanoparticles (MNPs) and their applications, which have shown the current project to be one of the major applications by laboratory results.METHODS: The nanoparticles synthesized in this project were deposited by the co-precipitation method, which structure was identified by analyzers such as SEM, FT-IR, and EDX. The aim of this project is the adsorption and fixation of biomolecule (BSA (bovine serum albumin) protein on the surface of magnetic nanoparticles.RESULTS: The adsorption results by electrophoresis and spectrophotometric analyzers showed an absorption rate above 55% ie; 55% of the protein is fixed on the MNPs nanoparticles. This absorption is due to the high level of functionality of magnetic nanoparticles for adsorption of protein. The results of the EDX analysis also show the possible electrostatic bonding between the nanoparticles and the protein, this is derived from –OH with –NH2 groups of the nanobiocompound (MNPs /protein). After bonding, the two are easily separated.CONCLUSION: In this project, the Fe3O4 nanoparticles was synthesized and identified by SEM, FT-IR, and EDX analyzers and finally reacted with the BSA protein (for the absorption of protein on MNPs) under experimental conditions at a standard temperature of 25° C. The results showed that about 55% of the protein was fixed on magnetic nanoparticles.

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