A validated silver-nanoparticle-enhanced chemiluminescence method for the determination of citalopram in pharmaceutical preparations and human plasma

Luminescence ◽  
2013 ◽  
Vol 29 (3) ◽  
pp. 266-274 ◽  
Author(s):  
Muhammad Naeem Khan ◽  
Muhammad Rasul Jan ◽  
Jasmin Shah ◽  
Sang Hak Lee
Keyword(s):  
Human Plasma ◽  
Silver Nanoparticle ◽  
Pharmaceutical Preparations ◽  
Chemiluminescence Method ◽  
Enhanced Chemiluminescence

Determination of sulpiride in pharmaceutical preparations and biological fluids using a Cr (III) enhanced chemiluminescence method

Luminescence ◽  
2012 ◽  
Vol 28 (6) ◽  
pp. 915-921 ◽  
Author(s):  
Muhammad Naeem Khan ◽  
Muhammad Rasul Jan ◽  
Jasmin Shah ◽  
Sang Hak Lee ◽  
Young Ho Kim
Keyword(s):  
Biological Fluids ◽  
Pharmaceutical Preparations ◽  
Chemiluminescence Method ◽  
Enhanced Chemiluminescence

A chemiluminescence method for the determination of verapamil hydrochloride in pharmaceutical and environmental samples based on an Fe3O4 nanoparticles enhanced NaHCO3–H2O2 system

2017 ◽  
Vol 9 (47) ◽  
pp. 6705-6712 ◽  
Author(s):  
Mortaza Iranifam ◽  
Nasim Rahmati Hendekhale
Keyword(s):  
Fe3o4 Nanoparticles ◽  
Environmental Samples ◽  
Chemiluminescence Method ◽  
Verapamil Hydrochloride ◽  
Enhanced Chemiluminescence ◽  
System P ◽  
H2o2 System

In this work, an Fe3O4 nanoparticles (NPs)-enhanced chemiluminescence (CL) method was developed for the determination of verapamil hydrochloride (VRP).

scholarly journals Determination of phenobarbital in human urine and serum usingflow injection chemiluminescence

2012 ◽  
Vol 58 (1) ◽  
pp. 88-94
Author(s):  
K. Li ◽  
L.Z. Nu ◽  
K.L. Khe ◽  
K.H. Song
Keyword(s):  
Human Urine ◽  
Pharmaceutical Preparations ◽  
Injection System ◽  
Chemiluminescence Method ◽  
Chemiluminescence Intensity ◽  
Complete Determination ◽  
Flow Injection System ◽  
Human Urine And Serum ◽  
Urine And Serum

A sensitive chemiluminescence method, based on the enhancive effect of phenobarbital on the chemiluminescence reaction between luminol and dissolved oxygen in a flow injection system, was proposed for the determination of phenobarbital. The chemiluminescence intensity responded to the concentration of phenobarbital linearly ranging from 0.05 to 10 ng⋅ml-1 with the detection limit of 0.02 ng⋅ml-1 (3σ). At a flow rate of 2.0 ml⋅min-1, a complete determination of phenobarbital, including sampling and washing, could be accomplished in 0.5 min, offering the sampling efficiency of 120 h-1 accordingly. The method was applied successfully in an assay of PB for pharmaceutical preparations, human urine and serum without any pretreatment with recovery from 95.7 to 106.7% and RSDs of less than 3.0%.

scholarly journals Spectrofluorometric Determination of Verapamil Hydrochloride in Pharmaceutical Preparations and Human Plasma Using Organized Media: Application to Stability Studies

2006 ◽  
Vol 89 (6) ◽  
pp. 1565-1572 ◽  
Author(s):  
Mohamed Walash ◽  
Fathalla Belal ◽  
Nahed El-Enany ◽  
Amina Abdelsalam
Keyword(s):  
Human Plasma ◽  
Biological Fluids ◽  
Sodium Dodecyl ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Official Method ◽  
Stability Studies ◽  
Verapamil Hydrochloride ◽  
Spiked Human Plasma

Abstract A highly sensitive spectrofluorometric method was developed for the determination of verapamil hydrochloride (VP HCl) in pharmaceutical formulations and biological fluids. The proposed method is based on investigation of the fluorescence spectral behavior of VP HCl in micellar systems, such as sodium dodecyl sulfate (SDS) and β-cyclodextrin (β-CD). In aqueous solutions of borate buffer of pH 9 and 8.5, VP HCl was well incorporated into SDS and β-CD, respectively, with enhancement of its native fluorescence. The fluorescence was measured at 318 nm after excitation at 231 nm. The fluorescence intensity enhancements were 183 and 107% in SDS and in β-CD, respectively. The fluorescence-concentration plots were rectilinear over the range of 0.020.2 and 0.020.25 μg/mL, with lower detection limits of 5.58 × 103 and 3.62 × 103 μg/mL in SDS and β-CD, respectively. The method was successfully applied to the analysis of commercial tablets and the results were in good agreement with those obtained with the official method. The method was further applied to the determination of VP HCl in real and spiked human plasma. The mean % recoveries in the case of spiked human plasma (n 4) was 92.59 3.11 and 88.35 2.55 using SDS and β-CD, respectively, while that in real human plasma (n 3) was 90.17 6.93 and 89.17 6.50 using SDS and β-CD, respectively. The application of the method was extended to the stability studies of VP HCl after exposureto ultraviolet radiation and upon oxidation with hydrogen peroxide.

scholarly journals Simultaneous Determination of Sitagliptin and Metformin in Pharmaceutical Preparations by Capillary Zone Electrophoresis and its Application to Human Plasma Analysis

2012 ◽  
Vol 7 ◽  
pp. ACI.S9940 ◽  
Author(s):  
Mohamed Salim ◽  
Nahed El-Enany ◽  
Fathallah Belal ◽  
Mohamed Walash ◽  
Gabor Patonay
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
Capillary Zone Electrophoresis ◽  
Pharmaceutical Preparations ◽  
Effective Length ◽  
Relative Standard ◽  
Significant Difference ◽  
Zone Electrophoresis ◽  
Capillary Zone

A novel, quick, reliable and simple capillary zone electrophoresis CZE method was developed and validated for the simultaneous determination of sitagliptin (SG) and metformin (MF) in pharmaceutical preparations. Separation was carried out in fused silica capillary (50.0 cm total length and 43.0 cm effective length, 49 μm i.d.) by applying a potential of 15 KV (positive polarity) and a running buffer containing 60 mM phosphate buffer at pH 4.0 with UV detection at 203 nm. The samples were injected hydrodynamically for 3 s at 0.5 psi and the temperature of the capillary cartridge was kept at 25 °C. Phenformin was used as internal standard (IS). The method was suitably validated with respect to specificity, linearity, limit of detection and quantitation, accuracy, precision, and robustness. The method showed good linearity in the ranges of 10-100 μg/mL and 50-500 μg/mL with limits of detection of 0.49, 2.11 μm/mL and limits of quantification of 1.48, 6.39 μg/mL for SG and MF, respectively. The proposed method was successfully applied for the analysis of the studied drugs in their synthetic mixtures and co-formulated tablets without interfering peaks due to the excipients present in the pharmaceutical tablets. The method was further extended to the in-vitro determination of the two drugs in spiked human plasma. The estimated amounts of SG/MF were almost identical with the certified values, and their percentage relative standard deviation values (% R.S.D.) were found to be ≤1.50% (n = 3). The results were compared to a reference method reported in the literature and no significant difference was found statistically.

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