Spectrofluorimetric determination of oseltamivir phosphate through derivatization witho-phthalaldehyde. Application to pharmaceutical preparations with a preliminary study on spiked plasma samples

Luminescence ◽  
2012 ◽  
Vol 27 (6) ◽  
pp. 511-518 ◽  
Author(s):  
M. I. Walash ◽  
F. Belal ◽  
N. El-Enany ◽  
M. H. El-Maghrabey
Keyword(s):  
Pharmaceutical Preparations ◽  
Plasma Samples ◽  
Spiked Plasma ◽  
Spectrofluorimetric Determination ◽  
Preliminary Study ◽  
Oseltamivir Phosphate

scholarly journals Spectrofluorimetric Determination of Vigabatrin and Gabapentin in Dosage Forms and Spiked Plasma Samples Through Derivatization with 4-Chloro-7-Nitrobenzo-2-Oxa-1,3-Diazole

2001 ◽  
Vol 84 (4) ◽  
pp. 1017-1024 ◽  
Author(s):  
Ekram M Hassan ◽  
Fathalla Belal ◽  
Omar A Al-Deeb ◽  
Nasr Y Khalil
Keyword(s):  
Reaction Pathway ◽  
Dosage Forms ◽  
Specific Method ◽  
Plasma Samples ◽  
Spiked Plasma ◽  
Highly Sensitive ◽  
Percent Recovery ◽  
Spectrofluorimetric Determination ◽  
Label Claim

Abstract A highly sensitive and specific method is proposed for the determination of vigabatrin (I) and gabapentin (II) in their dosage forms and spiked human plasma. The method is based on coupling the drugs with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole in borate buffer at pH 7.1 and measuring the resulting fluorescence at 532 nm after excitation at 465 nm. The fluorescence intensity was a linear function of the concentration of the drugs over the ranges of 1.3–6.5 and 1.7–8.5 μg/mL for I and II, respectively. Minimum detectability values were 0.54 μg/mL (4.2 × 10−6M) and 0.97 μg/mL (5.7 × 10−6M) for I and II, respectively, under the described conditions. The proposed method was successfully applied to the determination of the 2 drugs in their dosage forms, and the percent recoveries ± standard deviation (SD) were 104.53 ± 1.2 and 100.00 ± 1.32 of the label claim for I and II, respectively. The method was further applied to the determination of vigabatrin in spiked plasma samples. The percent recovery ± SD was 101.58 ± 2.68. Interference from endogenous α-amino acids was overcome through selective complexation with freshly prepared Cu(OH)2. The interference likely to be encountered from co-administered drugs, such as carbamazepine, cimetidine, clonazepam, clopazam, phenobarbital, valproic acid, and lamotrigine, was also studied. A reaction pathway is suggested.

scholarly journals Spectrofluorimetric Determination of Acetaminophen with N-Bromosuccinimide

2005 ◽  
Vol 88 (6) ◽  
pp. 1626-1630 ◽  
Author(s):  
Hanaa M Abdel-Wadood ◽  
Niveen A Mohamed ◽  
Fardous A Mohamed
Keyword(s):  
Pharmaceutical Preparations ◽  
Interference Effects ◽  
Fluorescent Intensity ◽  
Selective Method ◽  
Average Recovery ◽  
Spiked Plasma ◽  
Spectrofluorimetric Determination ◽  
Lower Detection ◽  
Stability Time

Abstract A simple, sensitive, and selective method for determination of acetaminophen based on its oxidation using N-bromosuccinimide (NBS) to produce a highly fluorescent product. Optimization of reaction variables was carried out concerning NBS concentration, pH, temperature, reaction time, and stability time. Under optimal analytical conditions, the fluorescent intensity was measured at λemission. 442 nm (excitation at λ 330 nm). The linearity range is 120–800 ng/mL with lower detection limit of 33.6 ng/mL acetaminophen. The method was applied successfully to the determination of the compound in pharmaceutical preparations, with average recovery of 100.3 ± 2%. The method was also applied successfully to the determination of the drug in spiked plasma samples, with an average recovery of 101.2 ± 1%. Interference effects of some compounds, present in combination with acetaminophen, were studied and the tolerance limits of these compounds were determined.

scholarly journals Combining derivative and synchronous approaches for simultaneous spectrofluorimetric determination of terbinafine and itraconazole

2020 ◽  
Vol 7 (8) ◽  
pp. 200571
Author(s):  
Heba Elmansi ◽  
Aya Roshdy ◽  
Shereen Shalan ◽  
Amina El-Brashy
Keyword(s):  
Biological Samples ◽  
Concentration Level ◽  
Detection Limits ◽  
Plasma Samples ◽  
International Conference ◽  
Spiked Plasma ◽  
Spectrofluorimetric Determination ◽  
Good Agreement

In this study, determination of terbinafine and itraconazole down to biological concentration level has been carried out. The determination is based on increasing the selectivity of the spectrofluorimetric technique by combining both derivative and synchronous spectrofluorometric approaches, which permits successful estimation of terbinafine at 257 nm and itraconazole at 319 nm in the presence of each other at Δ λ of 60 nm. International Conference on Harmonization validation guidelines were followed to fully validate the method, and linearity was obtained for the two drugs over the range of 0.1–0.7 µg ml −1 for terbinafine and 0.5–4.0 µg ml −1 for itraconazole. Application of the method was successfully carried out in the commercial tablets with good agreement with the comparison spectrofluorometric methods. As the detection limits were down to 0.013 and 0.1 µg ml −1 and quantitation limits were 0.04 and 0.032 µg ml −1 for terbinafine and itraconazole, respectively; the in vitro determination of terbinafine and itraconazole in spiked plasma samples was applicable. The percentage recoveries in biological samples were 97.17 ± 4.54 and 98.75 ± 2.25 for terbinafine and itraconazole, respectively. Water was used as the optimum diluting solvent in the proposed methodology which adds an eco-friendly merit.

RP-HPLC Method for Determination of Oseltamivir Phosphate in Capsules and Spiked Plasma

2010 ◽  
Vol 43 (14) ◽  
pp. 2200-2209 ◽  
Author(s):  
Zeynep Aydoğmuş ◽  
Sena Çağlar ◽  
Sıdıka Toker
Keyword(s):  
Hplc Method ◽  
Rp Hplc ◽  
Spiked Plasma ◽  
Oseltamivir Phosphate

scholarly journals Spectrofluorimetric Determination of Warfarin Sodium by Using N1-Methylnicotinamide Chloride as a Fluorigenic Agent

2005 ◽  
Vol 88 (2) ◽  
pp. 455-461 ◽  
Author(s):  
Mohamed A El Dawy ◽  
Mokhtar M Mabrouk ◽  
Riad A El Barbary
Keyword(s):  
Aqueous Solutions ◽  
Human Plasma ◽  
Plasma Samples ◽  
Spiked Human Plasma ◽  
Human Plasma Samples ◽  
Warfarin Sodium ◽  
Spectrofluorimetric Method ◽  
Spectrofluorimetric Determination ◽  
Methylene Groups

Abstract A spectrofluorimetric method is described for the determination of drugs containing active methylene groups adjacent to carbonyl groups. The method was applied successfully to the determination of warfarin sodium in laboratory-prepared mixtures, in commercial tablets, and in spiked human plasma samples. Finally, the method was applied to the determination of the steady-state concentration of warfarin sodium in the blood of a hospitalized patient. The method involves the reaction of warfarin sodium with 0.2 ml (0.4 × 10−3M) N1-methylnicotinamide chloride reagent in the presence of 3 mL 1.0N NaOH and cooling in ice for 8 min, followed by adjustment of the pH to 2.0, using formic acid and heating for 4 min, whereby a highly fluorescent reaction product is produced. The optimal wavelengths of excitation and emission were determined by using a synchronous wavelength search and found to be 284 and 354 nm, respectively. The standard curves were linear over a concentration range of 50–1500 ng/mL in both aqueous solutions and spiked human plasma samples. The mean recoveries (± standard deviation) were 101.157 (±1.33) and 95.73 (±1.88%) for aqueous solutions and spiked human plasma samples, respectively. The method showed good specificity and precision. The proposed method is simple and economical because of its minimal instrumentation and chemicals requirements. Nevertheless, it is highly sensitive, specific, and reproducible. Accordingly, it is suitable for quality-control applications, drug monitoring, and bioavailability and bioequivalency studies.

Spectrofluorimetric determination of clioquinol in pharmaceutical preparations

1991 ◽  
Vol 341 (10) ◽  
pp. 638-640 ◽  
Author(s):  
J. Gim�nez-Izquierdo ◽  
J. Guiteras ◽  
A. Izquierdo ◽  
M. D. Prat
Keyword(s):  
Pharmaceutical Preparations ◽  
Spectrofluorimetric Determination
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