Catalytic effect of ReAu nanoalloy on the Te particle reaction and its application to resonance scattering spectral assay of CA125

Luminescence ◽  
2010 ◽  
Vol 26 (5) ◽  
pp. 305-312 ◽  
Author(s):  
Wei Cai ◽  
Aihui Liang ◽  
Qingye Liu ◽  
Xianjiu Liao ◽  
Zhiliang Jiang ◽  
...  
Keyword(s):  
Catalytic Effect ◽  
Resonance Scattering ◽  
Resonance Scattering Spectral Assay

A new immune resonance scattering spectral assay for trace fibrinogen with gold nanoparticle label

2006 ◽  
Vol 571 (2) ◽  
pp. 200-205 ◽  
Author(s):  
Zhi-Liang Jiang ◽  
Shuang-Jiao Sun ◽  
Ai-Hui Liang ◽  
Chang-Jiu Liu
Keyword(s):  
Gold Nanoparticle ◽  
Resonance Scattering ◽  
Resonance Scattering Spectral Assay

A Highly Sensitive Aptamer-Nanogold Catalytic Resonance Scattering Spectral Assay for Melamine

2011 ◽  
Vol 21 (5) ◽  
pp. 1907-1912 ◽  
Author(s):  
Aihui Liang ◽  
Lianping Zhou ◽  
Huimin Qin ◽  
Yi Zhang ◽  
Huixiang Ouyang ◽  
...  
Keyword(s):  
Resonance Scattering ◽  
Resonance Scattering Spectral Assay ◽  
Highly Sensitive

scholarly journals Nanogold Catalysis–Based Immunoresonance-Scattering Spectral Assay for Trace Complement Component 3

2008 ◽  
Vol 54 (1) ◽  
pp. 116-123 ◽  
Author(s):  
Zhiliang Jiang ◽  
Wenxing Huang ◽  
Jiangping Li ◽  
Mingshun Li ◽  
Aihui Liang ◽  
...  
Keyword(s):  
Human Serum ◽  
Catalytic Effect ◽  
Linear Regression Analysis ◽  
High Sensitivity ◽  
Resonance Scattering ◽  
Complement Component ◽  
Serum Samples ◽  
Citrate Buffer ◽  
Nanogold Catalysis ◽  
Complement Component 3

Abstract Background: Complement component 3 (C3) is an essential bridge linking innate immunity and adaptive immunity. We describe an immunonanogold catalytic resonance-scattering (RS) technique for assaying C3 in serum. Methods: We used nanogold to label goat antihuman C3 antibody to obtain an immunonanogold RS probe for C3. The immune reaction between nanogold-labeled antibodies and antigens was carried out in Na2HPO4–sodium citrate buffer, pH 5.6, containing polyethylene glycol. After centrifuging the particle suspension, we used RS to monitor the catalytic effect of nanogold-labeled anti-C3 in the supernatant on the chlorauric acid–hydroxylamine (HAuCl4–NH2OH) particle reaction and used electron microscopy to monitor particle shape. We assayed 36 human serum samples with the immunonanogold catalytic RS assay and immunoturbidimetry. Results: Nanogold-labeled anti-C3 had a marked catalytic effect on the reaction of HAuCl4 and NH2OH to form particles, which exhibit a maximum RS peak at 585 nm. The decrease in RS intensity, ΔIRS, of the nanocatalytic system was proportional to C3 concentration from 5.0 to 160.0 ng/L. The detection limit for the C3 assay was 1.52 ng/L. Results obtained with serum samples agreed with those obtained with an immunoturbidimetric method. A linear regression analysis of 28 nonpathologic serum samples revealed a correlation coefficient of 0.960, with mean (SD) slope and intercept values of 0.787 (0.0218) g/L and 0.28 (0.026) g/L C3, respectively. Conclusion: The immunonanogold catalytic RS assay showed high sensitivity and good selectivity for measuring C3 in human serum. This method may become useful for diagnosing certain diseases, such as hepatitis.

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