Immunonanogold Catalytic Resonance Scattering Spectral Assay of Trace Immunoglobulin M

2008 ◽  
Vol 26 (8) ◽  
pp. 1417-1423 ◽  
Author(s):  
Ai-Hui LIANG ◽  
Su-Mei WANG ◽  
Zhi-Liang JIANG
2008 ◽  
Vol 13 (4) ◽  
pp. 302-308 ◽  
Author(s):  
Zhiliang Jiang ◽  
Lili Wei ◽  
Mingjing Zou ◽  
Aihui Liang ◽  
Mianwu Meng

Nanogold, 8 nm in size, was used to label goat antihuman immunoglobulin M (GIgM) to obtain a new immunonanogold resonance scattering (RS) probe (Au-GIgG) for quantitation of trace immunoglobulin M (IgM). The Au-GIgG combined with IgM to form nanogold-labeled immunocomplex causes the RS intensity at 580 nm to be enhanced, in pH 4.49 KH2PO 4-Na2HPO4 buffer and in the presence of polyethylene glycol 6000. The enhanced RS intensity at 580 nm (ΔI580 nm) is proportional to the IgM concentration in the range of 1.5 to 2000 ng/mL, with a lower detection limit of 0.98 ng/mL. The immunonanogold RS assay was used to assay IgM in serum samples, with sensitivity, selectivity, and simplicity. ( Journal of Biomolecular Screening 2008:302-308)


2006 ◽  
Vol 571 (2) ◽  
pp. 200-205 ◽  
Author(s):  
Zhi-Liang Jiang ◽  
Shuang-Jiao Sun ◽  
Ai-Hui Liang ◽  
Chang-Jiu Liu

2011 ◽  
Vol 21 (5) ◽  
pp. 1907-1912 ◽  
Author(s):  
Aihui Liang ◽  
Lianping Zhou ◽  
Huimin Qin ◽  
Yi Zhang ◽  
Huixiang Ouyang ◽  
...  

Plasmonics ◽  
2008 ◽  
Vol 3 (2-3) ◽  
pp. 73-78 ◽  
Author(s):  
Xiaoling Wei ◽  
Mingjing Zou ◽  
Zhiliang Jiang ◽  
Qingye Liu ◽  
Guiqing Wen

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