The ATP-bioluminescence method for a rapid evaluation of the microbial activity in the stone materials of monuments

1989 ◽  
Vol 3 (4) ◽  
pp. 213-216 ◽  
Author(s):  
Piero Tiano ◽  
Luisa Tomaselli ◽  
Claudio Orlando
Keyword(s):  
Microbial Activity ◽  
Rapid Evaluation ◽  
Stone Materials ◽  
Atp Bioluminescence ◽  
Bioluminescence Method

APPLICATION OF THE ATP-BIOLUMINESCENCE METHOD TO ASSESS THE QUALITY OF SANITATION OF MILKING EQUIPMENT

2020 ◽  
Vol 1 (4) ◽  
pp. 468-472
Author(s):  
P.A. Popov ◽  
◽  
D.V. Gruznov ◽  
S.V. Tokarev ◽  
◽  
...  
Keyword(s):  
Dairy Farms ◽  
Laboratory Studies ◽  
Before And After ◽  
Atp Bioluminescence ◽  
Microbial Number ◽  
Proportional Relationship ◽  
The Relationship ◽  
Number Of Bacteria ◽  
Bioluminescence Method

The article presents the results of determining the relationship between the total microbial number and microbial ATP on the surface of milking equipment before and after treatment with neutral anolyte ANK-SUPER. The possibility of using the ATP-bioluminescence method to control the quality of sanitation of milking equipment on dairy farms is shown. Laboratory studies revealed a proportional relationship between the level of bacterial ATP and the number of bacteria on the surface of milking equipment before and after sanitation.

scholarly journals Novel Antibiotic Susceptibility Tests by the ATP-Bioluminescence Method Using Filamentous Cell Treatment

1998 ◽  
Vol 42 (6) ◽  
pp. 1406-1411 ◽  
Author(s):  
Noriaki Hattori ◽  
Moto-O Nakajima ◽  
Koji O’Hara ◽  
Tetsuo Sawai
Keyword(s):  
Antimicrobial Agents ◽  
Susceptibility Testing ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Test Model ◽  
The Novel ◽  
Gram Negative ◽  
Atp Bioluminescence ◽  
Filamentous Cell ◽  
Bioluminescence Method

ABSTRACT Antimicrobial susceptibility testing by the ATP-bioluminescence method has been noted for its speed; it provides susceptibility results within 2 to 5 h. However, several disagreements between the ATP method and standard methodology have been reported. The present paper describes a novel ATP method in a 3.5-h test which overcomes these deficiencies through the elimination of false-resistance discrepancies in tests on gram-negative bacteria with β-lactam agents. In our test model using Pseudomonas aeruginosa and piperacillin, it was shown that ATP in filamentous cells accounted for the false resistance. We found that 0.5% 2-amino-2-methyl-1,3-propanediol (AMPD) extracted ATP from the filamentous cells without affecting normal cells and that 0.3 U of adenosine phosphate deaminase (APDase)/ml simultaneously digested the extracted ATP. We used the mixture of these reagents for the pretreatment of cells in a procedure we named filamentous cell treatment, prior to ATP measurements. This novel ATP method with the filamentous cell treatment eliminated false-resistance discrepancies in tests on P. aeruginosa with β-lactam agents, including piperacillin, cefoperazone, aztreonam, imipenem-cilastatin, ceftazidime, and cefsulodin. Furthermore, this novel methodology produced results which agreed with those of the standard microdilution method in other tests on gram-negative and gram-positive bacteria, including P. aeruginosa, Escherichia coli,Staphylococcus aureus, and Enterococcus faecalis, for non-β-lactam agents, such as fosfomycin, ofloxacin, minocycline, and aminoglycosides. MICs obtained by the novel ATP method were also in agreement with those obtained by the agar dilution method of susceptibility testing. From these results, it was shown that the novel ATP method could be used successfully to test the activities of antimicrobial agents with the elimination of the previously reported discrepancies.

Study on the Detection of Total Colony in Medical and Health Environment Based on ATP Bioluminescence

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
Zhe Li
Keyword(s):  
Culture Method ◽  
Pass Rate ◽  
Plate Count ◽  
Count Method ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Plate Count Method ◽  
The Difference ◽  
Atp Bioluminescence Assay ◽  
Bioluminescence Method

In this paper, the application of ATP fluorescence in the detection of colonies in the health environment of hospitals was studied. Firstly, the principle of ATP bioluminescence method was described. Then, ATP bioluminescence and plate count method were used to test the density of the surface of the objects in selected area, taking the time points 2 hours after disinfection as the time nodes. The results showed that the difference between the qualified rate of ATP bioluminescence assay and the plate count method was statistically significant {P<0.01}. Therefore, ATP bioluminescence method was highly correlated with bacterial culture method. The correlation coefficient of pass rate of the two methods was 0.782, which indicated that there was a positive correlation between the two test results. Besides, the detection results showed that ATP bioluminescence method had higher sensitivity than plate counting method. Therefore, ATP bioluminescence method was more suitable for the rapid detection of the colony of hospital health environment, and helps the hospital to better manage its environmental hygiene conditions. 

Application of ATP bioluminescence method to characterize performance of bioaerosol sampling devices

2009 ◽  
Vol 40 (2) ◽  
pp. 113-121 ◽  
Author(s):  
Satyanarayanan Seshadri ◽  
Taewon Han ◽  
Valdis Krumins ◽  
Donna E. Fennell ◽  
Gediminas Mainelis
Keyword(s):  
Atp Bioluminescence ◽  
Bioluminescence Method

Adenosine 5′-triphosphate and adenylate energy charge in sheep digesta

1982 ◽  
Vol 98 (3) ◽  
pp. 523-528 ◽  
Author(s):  
R. J. Wallace ◽  
Aileen A. West
Keyword(s):  
Microbial Activity ◽  
Metabolic Activity ◽  
Dry Matter ◽  
Alimentary Tract ◽  
Energy Charge ◽  
Adenylate Energy Charge ◽  
Large Decrease ◽  
Atp Content ◽  
Bioluminescence Method ◽  
Ciliate Protozoa

SUMMARYThe concentrations of adenosine 5′-mono-, -di-, and -triphosphate (AMP, ADP and ATP) in sheep digesta were measured by a new bioluminescence method. ATP in rumen digesta varied with time after feeding and diet, and most markedly according to the size of the population of ciliate protozoa: the ATP content of rumen digesta from ciliate-free sheep was less than a quarter of that of faunated animals receiving the same diet. The adenylate energy charge (EC) (ATP + ADP/[ATP + ADP + AMP]), an indicator of metabolic activity, was high (0·77–0·94) in all rumen samples, but did not appear to be as useful a measurement of activity as ATP alone. As digesta passed along the alimentary tract, the ATP content decreased progressively, from 0·9–2·2 μmol/g dry matter in the rumen to 0·5–1·0μmol/g in the abomasum and duodenum, 07middot;03–0·08 μmol/g in ileal digesta and 0·01–0·02 μmol/g in faeces. EC also tended to decrease, further emphasizing the large decrease in microbial activity which occurs in the hindgut.

Using ATP bioluminescence technique for monitoring microbial activity in sludge

2001 ◽  
Vol 75 (4) ◽  
pp. 469-474 ◽  
Author(s):  
C. P. Chu ◽  
D. J. Lee ◽  
Bea-Ven Chang ◽  
C. S. Liao
Keyword(s):  
Microbial Activity ◽  
Atp Bioluminescence

Development of a hygiene standard for raw milk based on the Lumac ATP-bioluminescence method

1996 ◽  
Vol 6 (7) ◽  
pp. 709-713 ◽  
Author(s):  
C. Bell ◽  
C.D. Bowles ◽  
M.J.K. Toszeghy ◽  
P. Neaves
Keyword(s):  
Raw Milk ◽  
Atp Bioluminescence ◽  
Bioluminescence Method

scholarly journals Paired design of ATP bioluminescence method and colony counting method: whether it is suitable for evaluating the disinfection effect of environmental surface?

2019 ◽  
Author(s):  
Huiqiong Xu ◽  
Jiansheng Liang ◽  
Yimei Wang ◽  
Bin Wang ◽  
Tianbao Zhang ◽  
...  
Keyword(s):  
Rank Correlation ◽  
Correlation Coefficients ◽  
Atp Content ◽  
Counting Method ◽  
Colony Counting ◽  
Significant Difference ◽  
Atp Bioluminescence ◽  
Environmental Surface ◽  
Paired Design ◽  
Bioluminescence Method

AbstractATP bioluminescence method has been used as a on-site rapid detection method in nosocomial infections control more and more. In this study, a paired design between four methods/detectors were performed to detect the environmental surface after disinfection. Two methods were ATP bioluminescence method and colony counting method (C), and ATP bioluminescence method included three kinds of detectors (B, P and N). Every surface was performed by two methods/detectors. ATP content per surface from ICU had no statistically significant difference with internal medicine wards of B and P, of which p was 0.435 and 0.260. By Spearman rank correlation coefficients, with the exception of better correlation between ATP content detected by B and P, the correlation between the remaining methods/detectors was poor or had no correlation. And the differences between detectors are statistically significant. Therefor, ATP bioluminescence method may not be suitable for the evaluation of disinfection effect, but be more appropriate for evaluating the cleanliness of environmental surface.

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